Team:Groningen/Notebook/Wetwork 12June2012

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E.coli transformed with Vio-biobrick (BBa_K274002)did not grow.
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To do:
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Repeated transformation of E.coli with:
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Grow transformed into liquid culture; check growth
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Vio (BBa_K274002)- Kanamycin resistant
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Red with pBAD promotor (BBa_K274120)- Ampicillin resistant
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Red without promotor (BBa_K274100) - Ampicillin restistant
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Red with constitutive promotor (BBa_K274110) - Ampicillin resistant
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Standard protocol for E.coli transformation used (see 11th of june).

Revision as of 15:10, 12 June 2012




E.coli transformed with Vio-biobrick (BBa_K274002)did not grow.

Repeated transformation of E.coli with:

Vio (BBa_K274002)- Kanamycin resistant Red with pBAD promotor (BBa_K274120)- Ampicillin resistant Red without promotor (BBa_K274100) - Ampicillin restistant Red with constitutive promotor (BBa_K274110) - Ampicillin resistant

Standard protocol for E.coli transformation used (see 11th of june).