Team:Groningen/Notebook/Wetwork 12June2012
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+ | E.coli transformed with Vio-biobrick (BBa_K274002)did not grow. | ||
- | + | Repeated transformation of E.coli with: | |
- | + | ||
+ | Vio (BBa_K274002)- Kanamycin resistant | ||
+ | Red with pBAD promotor (BBa_K274120)- Ampicillin resistant | ||
+ | Red without promotor (BBa_K274100) - Ampicillin restistant | ||
+ | Red with constitutive promotor (BBa_K274110) - Ampicillin resistant | ||
+ | |||
+ | Standard protocol for E.coli transformation used (see 11th of june). |
Revision as of 15:10, 12 June 2012
E.coli transformed with Vio-biobrick (BBa_K274002)did not grow.
Repeated transformation of E.coli with:
Vio (BBa_K274002)- Kanamycin resistant Red with pBAD promotor (BBa_K274120)- Ampicillin resistant Red without promotor (BBa_K274100) - Ampicillin restistant Red with constitutive promotor (BBa_K274110) - Ampicillin resistant
Standard protocol for E.coli transformation used (see 11th of june).