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Team:Groningen/Sticker
From 2012.igem.org
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.sticker2 { | .sticker2 { | ||
left: 340px; | left: 340px; | ||
| - | top: | + | top:1000px; |
position:absolute; | position:absolute; | ||
width: 130px; | width: 130px; | ||
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.Germination2 { | .Germination2 { | ||
left: 540px; | left: 540px; | ||
| - | top: | + | top:1000px; |
position:absolute; | position:absolute; | ||
width: 130px; | width: 130px; | ||
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.PBADmeat12 { | .PBADmeat12 { | ||
left: 340px; | left: 340px; | ||
| - | top: | + | top:1040px; |
position:absolute; | position:absolute; | ||
width: 130px; | width: 130px; | ||
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.PBADmeat22 { | .PBADmeat22 { | ||
left: 540px; | left: 540px; | ||
| - | top: | + | top:1040px; |
position:absolute; | position:absolute; | ||
width: 130px; | width: 130px; | ||
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.Pigments2 { | .Pigments2 { | ||
left: 340px; | left: 340px; | ||
| - | top: | + | top:1080px; |
position:absolute; | position:absolute; | ||
width: 130px; | width: 130px; | ||
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.Death2 { | .Death2 { | ||
left: 540px; | left: 540px; | ||
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position:absolute; | position:absolute; | ||
width: 130px; | width: 130px; | ||
Revision as of 14:01, 30 August 2012
The spores of Bacillus subtilis will be contained in a compartment which is impermeable to bacteria and liquid, but can still let through volatiles. Metabolites, water, and alanine should be separated from the spores by a breakable membrane. Mixing these compounds will cause germination.
The material we use for our sticker is TPX®, or polymethylpentene. This polymer is available as thin, transparent sheets. The advantage is that it is relatively cheap, strong, and capable of letting through volatiles. The radius of the pores in TPX® is 0.5 µm, which is 500x larger than the average badmeat-volatile, but still small enough to keep liquid and bacteria in (see the figure below).
Comparison of the size of the TPX pores, volatiles and Bacillus subtilis.
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