Team:Groningen/Sticker

From 2012.igem.org

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                 .sticker2 {
                 .sticker2 {
left: 340px;
left: 340px;
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top:800px;
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top:1000px;
position:absolute;
position:absolute;
                         width: 130px;
                         width: 130px;
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                 .Germination2 {
                 .Germination2 {
left: 540px;
left: 540px;
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top:1000px;
position:absolute;
position:absolute;
                         width: 130px;
                         width: 130px;
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                 .PBADmeat12 {
                 .PBADmeat12 {
left: 340px;
left: 340px;
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top:840px;
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top:1040px;
position:absolute;
position:absolute;
                         width: 130px;
                         width: 130px;
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                 .PBADmeat22 {
                 .PBADmeat22 {
left: 540px;
left: 540px;
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top:1040px;
position:absolute;
position:absolute;
                         width: 130px;
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                 .Pigments2 {
                 .Pigments2 {
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top:1080px;
position:absolute;
position:absolute;
                         width: 130px;
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                 .Death2 {
                 .Death2 {
left: 540px;
left: 540px;
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top:880px;
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top:1080px;
position:absolute;
position:absolute;
                         width: 130px;
                         width: 130px;

Revision as of 14:01, 30 August 2012




The Sticker

The spores of Bacillus subtilis will be contained in a compartment which is impermeable to bacteria and liquid, but can still let through volatiles. Metabolites, water, and alanine should be separated from the spores by a breakable membrane. Mixing these compounds will cause germination.

The material we use for our sticker is TPX®, or polymethylpentene. This polymer is available as thin, transparent sheets. The advantage is that it is relatively cheap, strong, and capable of letting through volatiles. The radius of the pores in TPX® is 0.5 µm, which is 500x larger than the average badmeat-volatile, but still small enough to keep liquid and bacteria in (see the figure below).

Comparison of the size of the TPX pores, volatiles and Bacillus subtilis.









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