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Team:University College London/Module 3
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== Description == | == Description == | ||
| - | As an alternative to '''Island Formation''' Modules (Modules 1 and 2), we are also developing an alternative solution – '''Degradation''' of plastic. This will focus on '''Polyethylene''' degradation, for which numerous bacteria and fungi are capable of degrading. Certain Multi-copper enzymes have been found to degrade Polyethylene and Polypropylene, and we are looking in particular at an enzyme called '''laccase''' has been found to degrade Polyethylene in a number of bacteria and fungi. | + | As an alternative to '''Island Formation''' Modules (Modules 1 and 2), we are also developing an alternative solution – '''Degradation''' of plastic. This will focus on '''Polyethylene''' degradation, for which numerous bacteria and fungi are capable of degrading. Certain Multi-copper enzymes have been found to degrade Polyethylene and Polypropylene, and we are looking in particular at an enzyme called '''laccase''' that has been found to degrade Polyethylene in a number of bacteria and fungi. |
The laccase system (BBa_K729002) will be driven by a strong '''constitutive''' promoter (BBa_J23119) which initiates the '''extracellular release''' of the laccase enzyme. Laccases in fact exhibit an ‘extraordinary’ range of substrates. It has conserved copper binding site that couple the '''oxidation reactions''' of a reducing susbtrate to the cleavage of a dioxygen bond. This series of oxidation reactions can be used for plastic '''degradation'''. | The laccase system (BBa_K729002) will be driven by a strong '''constitutive''' promoter (BBa_J23119) which initiates the '''extracellular release''' of the laccase enzyme. Laccases in fact exhibit an ‘extraordinary’ range of substrates. It has conserved copper binding site that couple the '''oxidation reactions''' of a reducing susbtrate to the cleavage of a dioxygen bond. This series of oxidation reactions can be used for plastic '''degradation'''. | ||
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Revision as of 14:03, 13 August 2012
Module 3: Degradation
Description | Design | Construction | Characterisation | Modelling | Results | Conclusions
Description
As an alternative to Island Formation Modules (Modules 1 and 2), we are also developing an alternative solution – Degradation of plastic. This will focus on Polyethylene degradation, for which numerous bacteria and fungi are capable of degrading. Certain Multi-copper enzymes have been found to degrade Polyethylene and Polypropylene, and we are looking in particular at an enzyme called laccase that has been found to degrade Polyethylene in a number of bacteria and fungi.
The laccase system (BBa_K729002) will be driven by a strong constitutive promoter (BBa_J23119) which initiates the extracellular release of the laccase enzyme. Laccases in fact exhibit an ‘extraordinary’ range of substrates. It has conserved copper binding site that couple the oxidation reactions of a reducing susbtrate to the cleavage of a dioxygen bond. This series of oxidation reactions can be used for plastic degradation.
