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Team:Groningen/Notebook/Wetwork 8August2012
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Result-> Measuring GFP in LB is not possible. Try again in m9 medium. | Result-> Measuring GFP in LB is not possible. Try again in m9 medium. | ||
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Phusion PCR reaction with violacein DNA, BBa_K274002.<br> | Phusion PCR reaction with violacein DNA, BBa_K274002.<br> | ||
Revision as of 14:03, 9 August 2012
Tom
diluting O/N ecoli culture with alsT promotor (150, 250, 300, 500) + GFP in LB to OD = 0.1. Grow for 2.5 hours. Dilute to an OD of 0.05. Place different liquid cultures in a 96 well plate for fluorescence GFP measurement.
Result-> Measuring GFP in LB is not possible. Try again in m9 medium.
Elbrich
Phusion PCR reaction with violacein DNA, BBa_K274002.
Nisa
Ligated fnr and lacI promoter with pigment(s) coding region and introduced the ligation product into E. coli DH5a
List of transformation:
- lacI promoter + amilCP (blue)
- lacI promoter + amilGFP (yellow)
- lacI promoter + lycopene (red)
- fnr promoter + amilCP
- fnr promoter + amilGFP
- fnr promoter + lycopene
- ) lacI promoter was induced by 0.2 and 0.5 mM IPTG
