Team:University College London/LabBook

From 2012.igem.org

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== Tuesday 24.7.12  ==
== Tuesday 24.7.12  ==
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Aim - Results of Transformation
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'''Aim - Results of Transformation'''
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'''Result:''' The table below indicates that there was growth for this transformation.  
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Result: The table below indicates that there was growth for this transformation.  
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{| class="wikitable"
 +
|-
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! colspan="2" |  Samples !! Volume Inoculated !! Colony Formation
 +
|-
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| rowspan="2" |BioBrick ||rowspan="2" | BBa_C0040  || 10ul || Yes
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|-
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| 90ul || Yes
 +
|-
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| rowspan="2" | Control || Positive (Contains BioBrick BBa_C0040)|| 36ul || Yes
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|-
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| Negative (No BioBrick)|| 36ul || No
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|-
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|}
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{| class="wikitable"
 +
|-
 +
! colspan="2" |  Samples !! Volume Inoculated !! Colony Formation
 +
|-
 +
| rowspan="2" |BioBrick ||rowspan="2" | BBa_C0040  || 10ul || Yes
 +
|-
 +
| 90ul || Yes
 +
|-
 +
| rowspan="2" | Control || Positive (Contains BioBrick BBa_C0040)|| 36ul || Yes
 +
|-
 +
| Negative (No BioBrick)|| 36ul || No
 +
|-
 +
|}

Revision as of 13:51, 31 July 2012

Monday 23.7.12

Aim - Transformation of TetR BBa_C0040 BioBrick

(LOGO) Transformation Protocol 1

Step 1 – Thawing Cells: Use W3100 cell line created in Week 2 (Expt 2.1)

Step 3 – Addition of BioBrick: To a 2ml eppendorf, add 1ul of the following BioBricks. Note: we have changed the protocol for our positive control. Previously it contained no BioBrick, but it has been recommended to us that we transform our positive control such that there is one for each BioBrick – this will tell us if the BioBrick has in any way affected cell viability. This will be used from this point onwards. Include an extra tube as a negative control, with no BioBrick added

Samples Function Module
BioBrick BBa_C0040 Tetracycline Repressor Buoyancy
Control Positive (Contains BioBrick BBa_C0040)
Negative (No Biobrick)

Step 9 - Plating samples on Agar Plates: The table below indicates the chosen inoculation volume (two for each BioBrick) and the correct gel antibiotic concentration for all samples.

Samples Volume Inoculated Antibiotic in Gel (ug/ml)
BioBrick BBa_C0040 10ul Ampicillin(50ug/ml)
90ul
Control Positive (Contains BioBrick BBa_C0040) 36ul No Antibiotic
Negative (No BioBrick) 36ul 2x Ampicillin(50ug/ml)


Tuesday 24.7.12

Aim - Results of Transformation Result: The table below indicates that there was growth for this transformation.

Samples Volume Inoculated Colony Formation
BioBrick BBa_C0040 10ul Yes
90ul Yes
Control Positive (Contains BioBrick BBa_C0040) 36ul Yes
Negative (No BioBrick) 36ul No
Samples Volume Inoculated Colony Formation
BioBrick BBa_C0040 10ul Yes
90ul Yes
Control Positive (Contains BioBrick BBa_C0040) 36ul Yes
Negative (No BioBrick) 36ul No


Hello World

bla bla bla


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