Team:Groningen/Notebook/Wetwork 23July2012

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Tom and Arjan started with gas chromatography mass spectrometry. Test sample: 5gr. fresh meat incubated @ 37C for 3 houres. For both column (HP5 and CP wax) three runs on each column with different incubation and syringe temperature (1: 80C, 100C 2: 100C, 120C 3: 120C, 140C)
Tom and Arjan started with gas chromatography mass spectrometry. Test sample: 5gr. fresh meat incubated @ 37C for 3 houres. For both column (HP5 and CP wax) three runs on each column with different incubation and syringe temperature (1: 80C, 100C 2: 100C, 120C 3: 120C, 140C)
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Nisa
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Plasmid isolation pSB1C3-alsT promoter + GFP
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NanoDrop result (ng/ul):
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 +
A1= 102.9
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A2= 96,6
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B1= 118,1
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B2= 113,7
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C1= 122,8
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C2= 88 Comment: gel picture OK!
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D1= 124,9
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D2= 93,3
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Result:
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expected size: 3300bp for the pSB1C3 (2079bp)+ alsT (~500bp)+ GFP (800bp)
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From the gel picture, only plasmid from colony C2 (promoter alsT300) showed the expected size. The other plasmids seem to be empty, probably due to self ligation
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Solution: Check plasmid from other white colony
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 +
redo transformation. Increase the ratio of plasmid backbone: insert gene

Revision as of 13:41, 25 July 2012





Tom and Arjan started with gas chromatography mass spectrometry. Test sample: 5gr. fresh meat incubated @ 37C for 3 houres. For both column (HP5 and CP wax) three runs on each column with different incubation and syringe temperature (1: 80C, 100C 2: 100C, 120C 3: 120C, 140C)


Nisa

Plasmid isolation pSB1C3-alsT promoter + GFP

NanoDrop result (ng/ul):

A1= 102.9

A2= 96,6

B1= 118,1

B2= 113,7

C1= 122,8

C2= 88 Comment: gel picture OK!

D1= 124,9

D2= 93,3


Result:

expected size: 3300bp for the pSB1C3 (2079bp)+ alsT (~500bp)+ GFP (800bp)

From the gel picture, only plasmid from colony C2 (promoter alsT300) showed the expected size. The other plasmids seem to be empty, probably due to self ligation

Solution: Check plasmid from other white colony

redo transformation. Increase the ratio of plasmid backbone: insert gene

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