From 2012.igem.org

Revision as of 16:23, 26 October 2012

Data For Our Favorite New Parts

1. BBa_K818000 - Integration vector: integrates into the chromosome of Bacillus subtilis at the SacA locus and replicates in Escherichia coli; derived from vector psac-cm (Middleton et al., 2004).
   
   
2. BBa_K818600 Rotting meat sensor system: contains the native Bacillus subtilis promoter PsboA (BBa_K818100) and yellow chromoprotein amilGFP (BBa_K592010) with RBS (BBa_B0034); PsboA is strongly activated by volatiles from rotten meat when B. subtilis grows exponentially. Produces a visible yellow color in six hours.
   
   

Data For Pre-existing Parts

1. BBa_K592010 Yellow chromoprotein amilGFP (Uppsala 2011): stable, non-toxic pigment, well perceptible in the visible and fluorescent spectrum. We characterized the working of the part in Bacillus subtilis for the first time.
   
   
2. BBa_K592009 Blue chromoprotein amilCP (Uppsala 2011): pigment visible by eye. We characterized visualization of the part in Bacillus subtilis for the first time.
   
   
3. BBa_823023 Integration vector (LMU Munich 2012): integrates into the Bacillus subtilis chromosome at the AmyE locus and replicates in E. coli. We confirmed that it works.
   
   

We've Also Characterized the Following Parts

1. BBa_K818100 Promoter PsboA, rotting meat sensor: natively activates production of the antibiotic compound biosubtilosin; weakly constitutive expressed at exponential growth, strongly upregulated when subjected to volatiles from spoiled meat.
   
   
2. BBa_K818400 Rotting meat sensor system: promoter PsboA (BBa_K818100) and blue chromoprotein amilCP (BBa_K592009) with RBS (BBa_B0034). Visible blue/purple color is obtained when using our sticker system.
   
   

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