Team:Groningen/Notebook/Wetwork 28June2012

From 2012.igem.org

(Difference between revisions)
Line 1: Line 1:
{{HeaderGroningen2012}}
{{HeaderGroningen2012}}
-
Tom
 
-
Cutting plasmid biobricks LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) with EcoR1 and loading them on an 0.8% agarose gel.
+
<html>
 +
<head>
 +
<style type="text/css">
 +
p.margin{
 +
font-size:14pt;
 +
line-height:14pt;
 +
color:white;
 +
margin-top:0px;
 +
margin-bottom:20px;
 +
margin-left:100px;
 +
margin-right:100px;
 +
}
 +
</style>
 +
</head>
-
Renske
+
<body>
 +
<p class="margin">
-
Growth curves with different start OD's. O/N culture of Bacillus subtilis 168 in LB diluted to OD(600) = 0.3 (2x: A1 & A2) and 0.4 (2x B1 & B2). It takes 2 hours to grow from OD(600) = 0.3 to OD(600) = 0.8.
+
Tom <br>
 +
Cutting plasmid biobricks LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) with EcoR1 and loading them on an 0.8% agarose gel.<br><br>
-
Micro-Array test run.
+
Renske <br>
-
Ran the setup made on 27th of june with a Bacillus subtilis culture (start OD(600) = 0.3, diluted from growth curve culture A1 (at OD(600) = 0.8)).  
+
 
-
After 2 hours running in the 37 degrees Celsius room, the OD(600) was 1.5. A bit too much! Tomorrow: start with OD(600) = 0.1.
+
Growth curves with different start OD's. O/N culture of Bacillus subtilis 168 in LB diluted to OD(600) = 0.3 (2x: A1 & A2) and 0.4 (2x B1 & B2). It takes 2 hours to grow from OD(600) = 0.3 to OD(600) = 0.8. <br><br>
 +
 
 +
 
 +
Micro-Array test run.<br>
 +
Ran the setup made on 27th of june with a Bacillus subtilis culture (start OD(600) = 0.3, diluted from growth curve culture A1 (at OD(600) = 0.8)). <br>
 +
After 2 hours running in the 37 degrees Celsius room, the OD(600) was 1.5. A bit too much! Tomorrow: start with OD(600) = 0.1. <br>
 +
 
 +
<br>
 +
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
 +
</p>
 +
</body>
 +
</html>
 +
 
 +
{{Template:SponsorsGroningen2012}}

Revision as of 16:49, 15 September 2012





Tom
Cutting plasmid biobricks LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) with EcoR1 and loading them on an 0.8% agarose gel.

Renske
Growth curves with different start OD's. O/N culture of Bacillus subtilis 168 in LB diluted to OD(600) = 0.3 (2x: A1 & A2) and 0.4 (2x B1 & B2). It takes 2 hours to grow from OD(600) = 0.3 to OD(600) = 0.8.

Micro-Array test run.
Ran the setup made on 27th of june with a Bacillus subtilis culture (start OD(600) = 0.3, diluted from growth curve culture A1 (at OD(600) = 0.8)).
After 2 hours running in the 37 degrees Celsius room, the OD(600) was 1.5. A bit too much! Tomorrow: start with OD(600) = 0.1.

Back to notebook

Our sponsors:

Retrieved from "http://2012.igem.org/Team:Groningen/Notebook/Wetwork_28June2012"