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Team:University College London/LabBook/Week14
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</div><div class="experiment"></div><img id="14-5" src="https://static.igem.org/mediawiki/2012/2/29/Ucl2012-graph14-5.png" /> | </div><div class="experiment"></div><img id="14-5" src="https://static.igem.org/mediawiki/2012/2/29/Ucl2012-graph14-5.png" /> | ||
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| - | ==14 | + | ==Friday 14.09.13== |
| + | |||
| + | |||
| + | '''Aim:''' To characterise nuclease activity | ||
| + | |||
| + | |||
| + | '''Method:''' | ||
| + | To add protocol | ||
| + | |||
| + | |||
| + | '''Results:''' | ||
| + | |||
| + | |||
| + | The following table shows readings of the diameters and OD at different time points: | ||
| + | |||
| + | |||
| + | {| class ="bigtable" | ||
| + | |- | ||
| + | ! Date !! Time !! Colony diameter/mm !! Halo diameter/mm !! Absorbance at 600 OD | ||
| + | |- | ||
| + | |Friday || 12.30 || 0 || 0 || 0 | ||
| + | |- | ||
| + | |Friday || 16.30 || 0 ||0 ||0 | ||
| + | |- | ||
| + | |Friday || 19.30 || 0 ||0 ||0 | ||
| + | |- | ||
| + | |Friday ||22.30 ||0 ||0 ||0 | ||
| + | |- | ||
| + | |Saturday ||01.30 ||0.5 ||1.5 ||0.068 | ||
| + | |- | ||
| + | |Saturday ||04.30 ||1 ||2 ||0.098 | ||
| + | |- | ||
| + | |Saturday ||07.30 ||1.5 ||3 ||0.159 | ||
| + | |- | ||
| + | |Saturday ||10.30 ||1.5 ||3.5 ||0.192 | ||
| + | |- | ||
| + | |Saturday ||12.30pm ||2 ||4 ||0.203 | ||
| + | |- | ||
| + | |Saturday ||14.30 ||2 ||4 ||0.209 | ||
| + | |- | ||
| + | |Saturday || 16.30 ||2.5 ||5 ||0.215 | ||
| + | |} | ||
| + | |||
| + | |||
| + | The average depth of the colonies was noted to be 0.5 - 1mm | ||
| + | |||
| + | |||
| + | '''Conclusion:''' works bust in comparision to one found naturally in wnu it produces less nucles, as expected. | ||
| + | |||
| + | |||
| + | |||
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<div class="experiment"></div></html> | <div class="experiment"></div></html> | ||
{{:Team:University_College_London/templates/foot}} | {{:Team:University_College_London/templates/foot}} | ||
Revision as of 00:27, 27 September 2012
Contents |
Monday 10.09.12
Aim: Day 2 of Generating competent cells. The aim is to incubate cells in the presence of CaCl2 to prepare the cell wall, such that it becomes permeable to DNA
Method: https://2012.igem.org/Team:University_College_London/Protocols/Competency2
Tuesday 11.09.12
Aim: Day 3 of Generating competent cells
Method: https://2012.igem.org/Team:University_College_London/Protocols/Competency3
14-2
14-3
14-4
Friday 14.09.13
Aim: To characterise nuclease activity
Method:
To add protocol
Results:
The following table shows readings of the diameters and OD at different time points:
| Date | Time | Colony diameter/mm | Halo diameter/mm | Absorbance at 600 OD |
|---|---|---|---|---|
| Friday | 12.30 | 0 | 0 | 0 |
| Friday | 16.30 | 0 | 0 | 0 |
| Friday | 19.30 | 0 | 0 | 0 |
| Friday | 22.30 | 0 | 0 | 0 |
| Saturday | 01.30 | 0.5 | 1.5 | 0.068 |
| Saturday | 04.30 | 1 | 2 | 0.098 |
| Saturday | 07.30 | 1.5 | 3 | 0.159 |
| Saturday | 10.30 | 1.5 | 3.5 | 0.192 |
| Saturday | 12.30pm | 2 | 4 | 0.203 |
| Saturday | 14.30 | 2 | 4 | 0.209 |
| Saturday | 16.30 | 2.5 | 5 | 0.215 |
The average depth of the colonies was noted to be 0.5 - 1mm
Conclusion: works bust in comparision to one found naturally in wnu it produces less nucles, as expected.
