Team:Goettingen/week9-3

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(Difference between revisions)
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<li>Observations & Results: <br>The corresponding Agarose gel showed bands at the expected fragment sizes (1721 bp for TAR_QC and 2048 bp for the vector backbone).
<li>Observations & Results: <br>The corresponding Agarose gel showed bands at the expected fragment sizes (1721 bp for TAR_QC and 2048 bp for the vector backbone).
Plasmids were labeled:<br>
Plasmids were labeled:<br>
-
pSB1C3_TAR_QC_18C<br>
+
pSB1C3_TAR_QC_18C Anderson promoter J23100; K777001<br>
-
pSB1C3_TAR_QC_18K<br>
+
pSB1C3_TAR_QC_18K Anderson promoter J23104; K777002<br>
-
pSB1C3_TAR_QC_18M<br>
+
pSB1C3_TAR_QC_18M Anderson promoter J23105; K777003<br>
-
pSB1C3_TAR_QC_18O<br>
+
pSB1C3_TAR_QC_18O Anderson promoter J23106; K777004<br>
-
pSB1C3_TAR_QC_20E<br>
+
pSB1C3_TAR_QC_2G  Anderson promoter J23109; K777005<br>
-
pSB1C3_TAR_QC_20G<br>
+
pSB1C3_TAR_QC_20E Anderson promoter J23112; K777006<br>
-
pSB1C3_TAR_QC_18I<br>
+
pSB1C3_TAR_QC_20G Anderson promoter J23113; K777007<br>
-
pSB1C3_TAR_QC_2G<br><br>
+
pSB1C3_TAR_QC_20I Anderson promoter J23114; K777008br><br>
These parts are submitted as BioBricks (see our <a href="https://2012.igem.org/Team:Goettingen/iGEM/Parts">submitted parts</a>).
These parts are submitted as BioBricks (see our <a href="https://2012.igem.org/Team:Goettingen/iGEM/Parts">submitted parts</a>).
</li>
</li>

Revision as of 23:29, 26 September 2012

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#3 Chemoreceptor Library - 9th Week

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V06_25


Ligation of promoterX_TAR_QC constructs into pSB1C3
  • Experiment:
    8 different promoter_TAR_QC constructs (for details see here) were ligated into the vector pSB1C3 according to protocol. Subsequently E. coli Δtar were transformed with the generated constructs, plated on CM plates and incubated at 37 °C overnight.


V06_28


V06_28_1 Preparation of pSB1C3_TAR_QC
  • Experiment:
    The vector pSB1C3 was prepped using the peqGOLD Plasmid Miniprep Kit (Peqlab) following the user maual. Elution in 50 µL elution buffer.

V06_28_2 Test digest of pSB1C3_TAR_QC
  • Experiment:
    A test digest of prepped pSB1C3_TAR_QC was performed according to protocol using XbaI and PstI. This should verify that the insertion of promoterX_TAR_QC into pSB1C3 has actually succeeded.
  • Observations & Results:
    The corresponding Agarose gel showed bands at the expected fragment sizes (1721 bp for TAR_QC and 2048 bp for the vector backbone). Plasmids were labeled:
    pSB1C3_TAR_QC_18C Anderson promoter J23100; K777001
    pSB1C3_TAR_QC_18K Anderson promoter J23104; K777002
    pSB1C3_TAR_QC_18M Anderson promoter J23105; K777003
    pSB1C3_TAR_QC_18O Anderson promoter J23106; K777004
    pSB1C3_TAR_QC_2G Anderson promoter J23109; K777005
    pSB1C3_TAR_QC_20E Anderson promoter J23112; K777006
    pSB1C3_TAR_QC_20G Anderson promoter J23113; K777007
    pSB1C3_TAR_QC_20I Anderson promoter J23114; K777008br>
    These parts are submitted as BioBricks (see our submitted parts).


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