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#3 Chemoreceptor Library - 9th Week

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V06_25

Ligation of promoterX_TAR_QC constructs into pSB1C3

Experiment:
8 different promoter_TAR_QC constructs (for details see here) were ligated into the vector pSB1C3 according to protocol. Subsequently E. coli Δtar were transformed with the generated constructs, plated on CM plates and incubated at 37 °C overnight.

V06_28

V06_28_1 Preparation of pSB1C3_TAR_QC

Experiment:
The vector pSB1C3 was prepped using the peqGOLD Plasmid Miniprep Kit (Peqlab) following the user maual. Elution in 50 µL elution buffer.

V06_28_2 Test digest of pSB1C3_TAR_QC

Experiment:
A test digest of prepped pSB1C3_TAR_QC was performed according to protocol using XbaI and PstI. This should verify that the insertion of promoterX_TAR_QC into pSB1C3 has actually succeeded.

Observations & Results:
The corresponding Agarose gel showed bands at the expected fragment sizes (1721 bp for TAR_QC and 2048 bp for the vector backbone). Plasmids were labeled:

Name	Anderson promoter	BioBrick
pSB1C3_TAR_QC_18C	J23100	K777001
pSB1C3_TAR_QC_18K	J23104	K777002
pSB1C3_TAR_QC_18M	J23105	K777003
pSB1C3_TAR_QC_18O	J23106	K777004
pSB1C3_TAR_QC_2G	J23109	K777005
pSB1C3_TAR_QC_20E	J23112	K777006
pSB1C3_TAR_QC_20G	J23113	K777007
pSB1C3_TAR_QC_20I	J23114	K777008

These parts are submitted as BioBricks (see our submitted parts).

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