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Team:Groningen/Notebook/Wetwork 10August2012
From 2012.igem.org
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Potassium: 0.2 g/L<br> | Potassium: 0.2 g/L<br> | ||
Glutamine: 0.1 g/L<br> | Glutamine: 0.1 g/L<br> | ||
| + | <br> | ||
| + | Yonathan<BR> | ||
| + | Liquid culture of promising pFNR+Lycopene colony had no growth. A few subcultures show no pink color, indicating an insert in the vector. Liquid cultures prepared of these colonies. | ||
<br> | <br> | ||
<br> | <br> | ||
Latest revision as of 18:36, 26 September 2012
Tom
E.Coli with alsT promotor and GFP (colonies: 150-1, 300-1, 300-2, 300-3, 300-4) were grown o/n in m9 medium, illuted to OD=0.1 and grown for 2.5 hours. Samples were prepared of OD=0.05 and divided in 96-well plate. GFP fluorescence was measured o/n.
Result:
No GFP was seen for all the samples.
Renske
Made minimal components medium based upon Jon's modeling data ("Jon's broth")
Glucose: 10 g/L
Potassium: 0.2 g/L
Glutamine: 0.1 g/L
Yonathan
Liquid culture of promising pFNR+Lycopene colony had no growth. A few subcultures show no pink color, indicating an insert in the vector. Liquid cultures prepared of these colonies.
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