Detection of LuxBrick’s Characteristic
In order to figure out the basic characteristic of k325909 submitted by Cambridge 2010, we measured growth curves of dH5a transformed k325909, which was induced by 0.3% arabinose (added from the very beginning of the incubation, and keep adding during the whole incubating process) under low temperature (25℃). As it shows in the chart, cells with and without induction grew to the logarithmic growth phase (about 5 hrs after inoculation) and stable phase (about 20 hrs after inoculation) were practically at the same time. But cells induced by arabinose displayed a significant decrease compared with those without induction.
Figure 1: growth curve of K325909 in a E.coli strain dH5a.