Team:Groningen/parts fail

From 2012.igem.org

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<z2>List of failed BioBricks</z2>
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<z2>List of failed BioBricks</z2><br>
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This is the list of unexpected BioBricks from 2012 distribution kit. <br>
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<b>BBa_K090403</b>
<b>BBa_K090403</b>

Revision as of 12:55, 26 September 2012





Failed Biobricks


List of failed BioBricks
This is the list of unexpected BioBricks from 2012 distribution kit.


BBa_K090403

Name: Gram-positive Shuttle Vector for Chromosomal Integration
Intended Purpose: Plasmid backbone for cloning in Bacillus subtilis
Testing Protocol: Restriction analysis (dual cut with EcoRI and PstI)
Results: The restriction pattern showed that this biobrick is not as described in the parts registry. The cut plasmid is not a single band and shorter than the expected size.
Gel Pic



BBa_I742123

Name: Multi-host vector pTG262 converted to BioBrick vector
Intended Purpose: Plasmid backbone for cloning in Bacillus subtilis
Testing Protocol: Restriction analysis (single cut) and restriction analysis (dual cut)
Results: The restriction enzymes cut at illegal site(s)
Gel Pic



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