Team:TMU-Tokyo/Notebook/Assay 1 Protocol and Result

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(Difference between revisions)

Revision as of 14:23, 26 September 2012

■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)

■Protocols

■Assay
Device1 Assay
Device2 Assay
Device3 Assay

Assay 1

■Assay1 Protocol

■Assay1 Result

@@ Line 55: / Line 55: @@
 <b>■Assay1 Protocol</b><Br>
 <Br><Br>
-I. Assay of resistance for formaldehyde<Br>
-. Make mediums<Br>
-Agar medium's recipe<Br>
-Agar mediums<Br>
-Dissolve parl  core agar medium 37.5 g into DW 200 ml.<Br>
-Autoclave for 20 minutes at 121 °C.<Br>
-<Br>
-Solution mediums<Br>
-Dissolve glucose 2 g, polypeptone 2 g and yeast extract 2 into DW 200 ml.<Br>
-Autoclave for 20 minutes at 121 °C.<Br>
-<Br>
-Phosphate buffer<Br>
-Make disodium hydrogen-phosphate aq 300 ml (0.2 M) and sodium<Br>
-dihydrogenphosphate dihydrate aq 300 ml (0.2 M) for pH 6.8.<Br>
-<Br>
-. Cultivate E. coli in solution mediums for overnight.<Br>
-. Prepare 1ml 0.1 %, 1 %, 5 % and 10 % solution of formaldehyde and formate.<Br>
-. Add 1 μl E. coli into these solutions for 10 minutes.<Br>
-. Plate on an agar medium.<Br>
-. Incubate at 37 °C for overnight.<Br>
-<Br>
-II. Additional experiment for formaldehyde<Br>
-. Prepare 1 ml 0.04 %, 0.08 %, 0.12 %, 0.16 % and 0.2 % of
-formaldehyde solution.<Br>
-. Add 1 μl E. coli into these solutions for 10 minutes.<Br>
-. Plate on an agar medium.<Br>
-. Incubate at 37 °C for overnight.<Br>