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Team:UNAM Genomics Mexico/Notebook/ANDSugar
From 2012.igem.org
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| + | <p class='captionInside'>1. 1 kb ladder <br /> | ||
| + | pHp45 Ω with E/P<br /> | ||
| + | We extracted from gel<br /> | ||
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<h2>06/15/12</h2><br /> | <h2>06/15/12</h2><br /> | ||
> Digested pHp45 Ω with E/P [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Digestion_protocol_.2820_.C2.B5l.29 | LIQUID CULTURE]]. <br /> | > Digested pHp45 Ω with E/P [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Digestion_protocol_.2820_.C2.B5l.29 | LIQUID CULTURE]]. <br /> | ||
Revision as of 04:59, 23 September 2012
Contents |
Arabinose/Xylose AND Gate
| Nanotubes!! | The logic | Random info |
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1. 1 kb ladder
2.E1010
06/07/12
PY BROTH 10g salt/L
PSB2K3 kanamicin
We transformed RFP E1010.
plate 1 18F
plate 2 17E
Stock 50 mg/mL
E.coli 1/1000
TRANSFORMATION PROTOCOL.
We made liquid cultures with colonies LIQUID CULTURE.
We extracted plasmids PLASMID EXTRACTION PROTOCOL.
We ran a gel to check the extraction GEL ELECTROPHORESIS PROTOCOL.
06/12/12
We made glycerols of the bacteria
GLYCEROL PROTOCOL .
We transformed plasmid pHp45 TRANSFORMATION PROTOCOL.
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1. 1 kb ladder
2.E1010
06/13/12
We ran gel and extracted from gel LIQUID CULTURE.
We made liquid cultures LIQUID CULTURE and lysed [LYSIS PROTOCOL].
-
1. 1 kb ladder
We extracted from gel
06/14/12
We extracted from gel LIQUID CULTURE.
We made glycerols of pHp45 Ω GLYCEROL PROTOCOL .
We transformed PSB2K3 Km (Kanamycin) 5C BBa_J04450 PSB4A5 Am (ampicillin) 1I BBa_J04450 AraC BBa_C0080
2012 14L plate 1 pSB2K3 Km+
2011 14L plate 1 pSB2K3 Km+
2012 14L plate 1 pSB2K3 Km+
TRANSFORMATION PROTOCOL.
-
1. 1 kb ladder
pHp45 Ω with E/P
We extracted from gel
06/15/12
> Digested pHp45 Ω with E/P LIQUID CULTURE.
> We extracted plasmid with kit.
> Ran a gel GEL ELECTROPHORESIS PROTOCOL . (5)
>Extracted from gel LIQUID CULTURE.
ARAC
>From the overnight plate we prepared liquid culture LIQUID CULTURE.
>We left them incubating overnight.
>We left a plate (LB Km DH5 C0080 and a control).
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