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Team:UNAM Genomics Mexico/Notebook/Protocols
From 2012.igem.org
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| - | <center>< | + | <center><h1.NORM_header>Protocols</h1.NORM_header></center> |
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| + | <Center><h1>E. coli Protocols</h1></center> | ||
| + | <br /> | ||
| + | <h2>PCR PROTOCOL (50µl)</h2><br /> | ||
| + | Buffer Pfu 5 µl<br /> | ||
| + | Upper primers 2.5 µl<br /> | ||
| + | Lower primers 2.5 µl<br /> | ||
| + | MgCL2 1 µl<br /> | ||
| + | DNA (depends on concentration, usually 0.5µl) <br /> | ||
| + | Enzime Taq Polymerase 1 µl<br /> | ||
| + | DNTP’s 8 µl<br /> | ||
| + | Water 29.5 µl<br /><br /> | ||
| + | Cycles :) <br /> | ||
| + | 95ºC 4 min<br /> | ||
| + | 95ºC 1 min <br /> | ||
| + | 55ºC :30 secs<br /> | ||
| + | 70ºC 1 min<br /> | ||
| + | goto 2:4 30 times<br /> | ||
| + | 72ºC 5 min<br /><br /> | ||
Revision as of 07:30, 14 September 2012
Protocols
E. Coli Protocols |
|
Bacillus Protocols |
E. coli Protocols
PCR PROTOCOL (50µl)
Buffer Pfu 5 µl
Upper primers 2.5 µl
Lower primers 2.5 µl
MgCL2 1 µl
DNA (depends on concentration, usually 0.5µl)
Enzime Taq Polymerase 1 µl
DNTP’s 8 µl
Water 29.5 µl
Cycles :)
95ºC 4 min
95ºC 1 min
55ºC :30 secs
70ºC 1 min
goto 2:4 30 times
72ºC 5 min
