Team:OUC-China

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<h1>Our climb will be steep<br/>吾将上下而求索！</h1>
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                    <div class="feature-slide-preview" style="display:none;">
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<li> <a href="#">Index</a> </li>
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                        <a href="#" title="Floating bacteria" target="_blank">
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<li> <a href="https://2012.igem.org/Team:OUC-China/Project/Overview">Project</a> </li>
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                            <img alt="Floating bacteria" title="Floating bacteria" src="https://static.igem.org/mediawiki/igem.org/a/a5/OUC-slide_box_1.jpg" />
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<li> <a href="https://2012.igem.org/Team:OUC-China/Modeling/Overview">Modeling</a> </li>
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                        </a>
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<li> <a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Overview">Human Practice</a> </li>
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                    </div>
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<li> <a href="https://2012.igem.org/Team:OUC-China/Team/members">Team</a> </li>
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<li> <a href="https://2012.igem.org/Team:OUC-China/Parts">Parts</a> </li>
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                        <a href="" title="Description of our project" target="_blank">
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<li> <a href="https://2012.igem.org/Team:OUC-China/Safety">Safety</a> </li>
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                            <img alt="Description of our project" title="Description of our project" src="https://static.igem.org/mediawiki/igem.org/3/3a/OUC-slide_box_2.jpg" />
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<li> <a href="https://2012.igem.org/Team:OUC-China/labnote">Note</a> </li>
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                        </a>
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</ul>
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                    </div>
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                        </a>
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<a href="https://2012.igem.org/Team:OUC-China">
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                    </div>
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Home
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<a href="https://2012.igem.org/Team:OUC-China#hmo">
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</div>
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<img src="https://static.igem.org/mediawiki/2012/4/49/Humanpracticeoverview.jpg" />
 +
HumanPractice index
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</a>
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</li>
 +
<li>
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<a href="https://2012.igem.org/Team:OUC-China/Judgingform">
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<img src="https://static.igem.org/mediawiki/2012/6/6e/Juding-form.jpg" />
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JudgingForm
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</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/contactus">
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<img src="https://static.igem.org/mediawiki/2012/1/1e/Contact-us.jpg" />
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Contact Us
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<ul class="submenu">
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<img src="https://static.igem.org/mediawiki/2012/5/59/Ouc-nav-project-overview.jpg" />
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Project Overview
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</a>
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</li>
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<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/Project/Sensor/AbstractandBackground">
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<img src="https://static.igem.org/mediawiki/2012/1/15/Ouc-nav-project-secsor.jpg" />
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Sensor
 +
</a>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract">
 +
<img src="https://static.igem.org/mediawiki/2012/3/34/Ouc-nav-device.jpg" />
 +
Decision-making Device
 +
</a>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground">
 +
<img src="https://static.igem.org/mediawiki/2012/5/57/Ouc-nav-project-gvp.jpg" />
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Gas vesicle
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</a>
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</li>
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<ul class="submenu">
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 +
ODEModel
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</a>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/Modeling/ParameterSensitivityAnalysis">
 +
<img src="https://static.igem.org/mediawiki/2012/c/cf/Ouc-nav-modeling-sensertitive.jpg" />
 +
Parameter Sensitivity Analysis
 +
</a>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/Modeling/ParameterSweep">
 +
<img src="https://static.igem.org/mediawiki/2012/0/07/Ouc-nav-modeling-sweep.jpg" />
 +
Parameter Sweep
 +
</a>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/Modeling/NoiseAnalysis">
 +
<img src="https://static.igem.org/mediawiki/2012/0/04/Ouc-nav-modeling-noise.jpg" />
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Noise Analysis
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</a>
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</li>
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</ul>
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<ul class="submenu">
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Overview">
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<img src="https://static.igem.org/mediawiki/2012/6/61/Ouc-nav-humanoverview.jpg" />
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HumanPractice Overview
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</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication">
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<img src="https://static.igem.org/mediawiki/2012/c/cc/Ouc-nav-Communication.jpg" />
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Meeting and Academic Communication
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</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps">
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<img src="https://static.igem.org/mediawiki/2012/8/8d/Ouc-nav-humcamp.jpg" />
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Camps, Class and Lectures
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</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM">
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<img src="https://static.igem.org/mediawiki/2012/b/b7/Ouc-nav-hmspcial.jpg" />
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Special HP
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</a>
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</li>
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<ul class="submenu">
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<a href="https://2012.igem.org/Team:OUC-China/Team/members">
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<img src="https://static.igem.org/mediawiki/2012/6/6d/Ouc-nav-team.jpg" />
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Team Members
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</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/Team/instructor">
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<img src="https://static.igem.org/mediawiki/2012/3/35/Ouc-nav-instructor.jpg" />
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Instructors
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</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/Team/AcknowledgementandCooperation">
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<img src="https://static.igem.org/mediawiki/2012/a/a6/Ouc-nav-co.jpg" />
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Acknowledgement&Cooperation
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</a>
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</li>
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/Team/Lab">
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Lab
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Parts
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</a>
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<ul class="submenu">
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<a href="https://2012.igem.org/Team:OUC-China/Safety">
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<img src="https://static.igem.org/mediawiki/2012/5/5d/Ouc-nav-safety.jpg" />
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Safety
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</a>
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</li>
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</ul>
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<ul class="submenu">
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<li>
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<a href="https://2012.igem.org/Team:OUC-China/labnote">
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<img src="https://static.igem.org/mediawiki/2012/8/8b/Ouc-nav-labnote.jpg" />
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Labnote
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</a>
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</li>
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<li>
 +
<a href="https://skydrive.live.com/redir?resid=6A13E80E2A176A23!249&authkey=!AGsNOi9excznIt8">
 +
<img src="https://static.igem.org/mediawiki/2012/4/4e/Ouc-nav-modelingnote.jpg" />
 +
Modeling Note
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</a>
 +
</li>
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<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/protocol">
 +
<img src="https://static.igem.org/mediawiki/2012/7/7d/Ouc-pro.png" />
 +
Protocols
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</a>
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</li>
 +
</ul>
</div>
</div>
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<div class="text_box">
+
<div class="container">
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<h2>Description of our project</h2>
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<div id="da-slider" class="da-slider">
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<p> 猫扑的创始人好像是湖南电信的员工，所以有服务器、带宽资源可以利用，最早其实是专门讨论游戏的论坛，讨论的人多了，自然也越来越杂了。
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<div class="da-slide">
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      2000、2001年那时候的"猫扑大杂烩"BBS，只有一个版块，不区分话题，左右分栏，一个网页内可以浏览各种千奇百怪的话题，这一点上要比Discuz那种版块式的更聚集人气和内容。最早接触网络的一批人都泡在上面，再加上各种电脑杂志的推荐，使得越来越多的新"网虫"慕名来到猫扑。
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<h2 >Overview</h2>
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      当时同样比较火的论坛还有西祠胡同等，但猫扑在论坛功能上的创新是其成功的关键，包括论坛货币系统MP（Mop Power）、表情（经典的253、874号GIF表情图）、道具（置顶、标题加粗加亮、隐身、提升下沉……非常丰富有趣，甚至论坛会员之间可以互相整蛊）、答题系统等等，我觉得是一个很大很大的亮点。
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<p>Red tide detector:
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      同时，当时论坛的讨论气氛非常好，各种网络热点话题大多都是出自猫扑，网络红人ayawawa就是在猫扑上红起来的。此外，悬赏帖也多，赏金猎人们活跃，这多少也归功于猫扑币的价值，可以换各大网游的点卡、买5位6位无保QQ号，甚至还可以直接卖给别人兑换成RMB，对网民的吸引力从中可见一斑。
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Facilitate small RNA as information processing media;
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<br/>
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and gas vesicle as a novel output actuator...
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<br/>
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Response to the overloading phosphate or nitrate when they exceeds warning limit...<br/>
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</p>
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<a href="https://2012.igem.org/Team:OUC-China/Project/Overview" class="da-link">Read more</a>
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<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/1/19/Ouc-final.png" alt="overview" /></div>
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</div>
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<div class="da-slide">
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<h2>Sensor</h2>
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<p>The sensors for next-generation detector of red tide!</p>
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<a href="https://2012.igem.org/Team:OUC-China/Project/Sensor/AbstractandBackground" class="da-link">Read more</a>
 +
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/3/32/Home-sensor.png" alt="sensor" /></div>
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</div>
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<div class="da-slide">
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<h2>Model-Driven Design</h2>
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<p>
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Get specific directions for ternary system design from simulation...
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</p>
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<a href="https://2012.igem.org/Team:OUC-China/Modeling/Overview" class="da-link">Read more</a>
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<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/2/26/Home-modeling.png" alt="Home-modeling" /></div>
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</div>
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<div class="da-slide">
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<h2>Making decision through sRNA</h2>
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<p>The sophisticated  'CPU' of our red tide detector...<br/>
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Engineering CPU for your own "synthia"!</p>
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<a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract" class="da-link">Read more</a>
 +
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/2/2c/Home-decision-making-device.png" alt="Home-decision-making-device" /></div>
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</div>
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      自从04年被千橡收购了之后，就没有了之前的那个味道，变成90后的阵地了，物是人非。</p>
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<div class="da-slide">
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</div>
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<h2>Floating Device</h2>
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<div class="sc_menu_wrapper">
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<p>The output actuator of our red tide detector...<br/>
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<div class="sc_menu">
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Gas vesicle as our floating device</p>
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    <a title="Menu" href=""><img src="images/1.jpg" alt="Menu"/></a>
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<a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground" class="da-link">Read more</a>
-
    <a title="Navigation" href=""><img src="images/2.jpg" alt="Navigation"/></a>
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<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/a/a2/Ouc-home-floating-device.png" /></div>
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<a title="jQuery" href=""><img src="images/3.jpg" alt="jQuery"/></a>
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</div>
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<nav class="da-arrows">
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<span class="da-arrows-prev"></span>
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<span class="da-arrows-next"></span>
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</nav>
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</div>
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        </div>
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<div id="project_intro_box">
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<div class="project_intro">
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<h2>Oceanfloat and Oceanfeel<br/><span>——a floating biosensor</span></h2>
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</div>
</div>
</div>
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<section id="project_step">
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<div class="moduletable">
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<h3>Our Project</h3>
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<div>
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<ul id="tab">
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<li class="on" id="tab_1" onclick="switchTab(1)"><img src="https://static.igem.org/mediawiki/2012/8/8f/Ouc-home-step1.png" /><span>Part1</span></li>
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<li id="tab_2" onclick="switchTab(2)"><img src="https://static.igem.org/mediawiki/2012/d/de/Ouc-home-step3.png" /><span>Part2</span></li>
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<li id="tab_3" onclick="switchTab(3)"><img src="https://static.igem.org/mediawiki/2012/a/a4/Ouc-home-steplast.png" /><span>Part3</span></li>
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</ul>
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<div style="clear: both;">&nbsp;</div>
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<div class="tuxing"><img src="https://static.igem.org/mediawiki/2012/e/e2/Ouc-hometab_arrow.png" /></div>
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<ul id="tab_con">
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<li id="tab_con_1">
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<div class="tup"><img src="https://static.igem.org/mediawiki/2012/3/3a/Ouc-home-step1-2.png" /></div>
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<div class="nr">
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<h1>Sensor</h1>
 +
<p>Our phosphate- and nitrate-sensitive devices are responsible for detection of environmental phosphate and nitrate. Besides that they transform the input signals into "readable information" for decision-making devices.</p>
 +
<p>much work has been done for characterization and modification of nitrate- and phosphate-sensitive promoters. A whole set of design ideas for TCS modifications are also presented for other igemers. Additionally an actuator that can remove the phosphate is recommended.</p>
 +
<p><a href="https://2012.igem.org/Team:OUC-China/Project/Sensor/AbstractandBackground"><strong>learn more</strong></a>
 +
</div>
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<div style="clear: both;">&nbsp;</div>
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</li>
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<li id="tab_con_2">
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<div class="tup"><img src="https://static.igem.org/mediawiki/2012/a/a1/Ouc-home-step3-02.png" /></div>
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<div class="nr">
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<h1>Decision-making Device</h1>
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<p>Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.</p>
 +
<p>Both comparator and ratio sensor facilitate a ternary system consisted of two small RNA and GFP mRNA. The concentration change of external two inducers leads to the transcription rate change of small RNA(alpha s)in this ternary system, the interactions between three RNAs will lead to interesting behaviors of our system like sensing the difference between two inputs quantitatively(Comparator) or the ratio of two inputs(Ratio senor).</p>
 +
<p><a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract"><strong>learn more</strong></a>
 +
</div>
 +
<div style="clear: both;">&nbsp;</div>
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</li>
 +
<li id="tab_con_3">
 +
<div class="tup"><img src="https://static.igem.org/mediawiki/2012/f/fa/Ouc-step3-last.png" /></div>
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<div class="nr">
 +
<h1>Floating Device</h1>
 +
<p>In our project, by transforming the buoyancy device BBa_K737010(designed by ourselves), the gas vesicle cluster from the genome of Planktothrix rubescens into E.Coli, we succeed in making Escherichia.Coli floating on the surface of the water. It can help the cells produce the gas vesicle inside, which could make densities significant stratification in the culture. Not only the gene cluster but the two parts BBa_K737006 and BBa_K737007, which produce the gas vesicle structure protein, can also make the Escherichia.Coli floating. We use the shorter length of the part achieve a better effect than BBa_K190033 and the gas vesicle application prospect.</p>
 +
<p><a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground"><strong>learn more</strong></a>
</div>
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<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication" title="Meeting and Academic Communication" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/d7/Ouc-homehp01.jpg" alt="Meeting and Academic Communication" title="Meeting and Academic Communication" /></a>
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<p>As a new born iGEM team,questions below puzzled us for a very long time. However,Peking Univerisity iGEM team helped us a lot by giving us dire……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication" title="Meeting and Academic Communication" target="_blank">[Learn more]</a></span></p>
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<p>Last year, we held 1st summer camp of life science and technology. Through this camp, most of the participants……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps" title="Camps, Class and Lectures" target="_blank">[Learn more]</a></span></p>
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<p>Because our project of this focuses on the ratio of N and P and cyanobacteria in the sea water, we consulted professors who study on ecology……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM" title="Special OUC-China HP" target="_blank">[Learn more]</a></span></p>
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Latest revision as of 13:25, 26 October 2012

Overview

Red tide detector: Facilitate small RNA as information processing media;
and gas vesicle as a novel output actuator...
Response to the overloading phosphate or nitrate when they exceeds warning limit...

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overview

Sensor

The sensors for next-generation detector of red tide!

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sensor

Model-Driven Design

Get specific directions for ternary system design from simulation...

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Home-modeling

Making decision through sRNA

The sophisticated 'CPU' of our red tide detector...
Engineering CPU for your own "synthia"!

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Home-decision-making-device

Floating Device

The output actuator of our red tide detector...
Gas vesicle as our floating device

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Oceanfloat and Oceanfeel
——a floating biosensor

Our Project

  • Part1
  • Part2
  • Part3
 

  • Sensor

    Our phosphate- and nitrate-sensitive devices are responsible for detection of environmental phosphate and nitrate. Besides that they transform the input signals into "readable information" for decision-making devices.

    much work has been done for characterization and modification of nitrate- and phosphate-sensitive promoters. A whole set of design ideas for TCS modifications are also presented for other igemers. Additionally an actuator that can remove the phosphate is recommended.

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  • Decision-making Device

    Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.

    Both comparator and ratio sensor facilitate a ternary system consisted of two small RNA and GFP mRNA. The concentration change of external two inducers leads to the transcription rate change of small RNA(alpha s)in this ternary system, the interactions between three RNAs will lead to interesting behaviors of our system like sensing the difference between two inputs quantitatively(Comparator) or the ratio of two inputs(Ratio senor).

    learn more

     

  • Floating Device

    In our project, by transforming the buoyancy device BBa_K737010(designed by ourselves), the gas vesicle cluster from the genome of Planktothrix rubescens into E.Coli, we succeed in making Escherichia.Coli floating on the surface of the water. It can help the cells produce the gas vesicle inside, which could make densities significant stratification in the culture. Not only the gene cluster but the two parts BBa_K737006 and BBa_K737007, which produce the gas vesicle structure protein, can also make the Escherichia.Coli floating. We use the shorter length of the part achieve a better effect than BBa_K190033 and the gas vesicle application prospect.

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Human practice


Retrieved from "http://2012.igem.org/Team:OUC-China"