Team:Groningen/Notebook/Wetwork 28August2012

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Tom
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Redo the ligation of Fnr promotor and alsT300 promotor with lycopene and GFP in Psac.
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Result: Incubation temperature of the ligation was too high resulting in no colonies after transformation.
 
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5 overnight cultures of each previous transformation, 26August2012, were incubated.
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Tom<br>
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Redo the ligation of Fnr promotor and alsT300 promotor with lycopene and GFP in Psac.<br>
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<br>
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Result: Incubation temperature of the ligation was too high resulting in no colonies after transformation.<br>
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Tom/Arjan<br>
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5 overnight cultures of each previous transformation, 26August2012, were incubated.<br>
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Result: All the colonies contained empty Psac plasmid.<br>
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Result: All the colonies contained empty Psac plasmid.
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Latest revision as of 13:49, 26 September 2012







Tom

Redo the ligation of Fnr promotor and alsT300 promotor with lycopene and GFP in Psac.

Result: Incubation temperature of the ligation was too high resulting in no colonies after transformation.


Tom/Arjan

5 overnight cultures of each previous transformation, 26August2012, were incubated.

Result: All the colonies contained empty Psac plasmid.


Back to notebook

Our sponsors:

Retrieved from "http://2012.igem.org/Team:Groningen/Notebook/Wetwork_28August2012"