Team:Groningen/Notebook/Wetwork 20June2012

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Bacillus 168 strain provided by Jan-Willem Veening. Grown overnight on LB agar plate at 37C.
 
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Bacillus 168 strain provided by Jan-Willem Veening. Grown overnight on LB agar plate at 37C.
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Plasmid isolation of bacillus backbone for chromosomal integration (BBa_K090403, size: 5632 bp) and terminator biobricks (BBa_B0020 and BBa_B0012, both size: 2058 bp) using the Roche High Pure Plasmid Isolation Kit.
Plasmid isolation of bacillus backbone for chromosomal integration (BBa_K090403, size: 5632 bp) and terminator biobricks (BBa_B0020 and BBa_B0012, both size: 2058 bp) using the Roche High Pure Plasmid Isolation Kit.
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Plasmid concentrations after isolation: <br>
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> BBa_K090403: 50.8 ng/uL, 260/280 = 1.85 <br>
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Plasmid concentrations after isolation:  
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> BBa_B0020: 93.5 ng/uL, 260/280 = 1.84 <br>
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> BBa_K090403: 50.8 ng/uL, 260/280 = 1.85
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> BBa_ B0012: 60.0 ng/uL, 260/280 = 1.93 <br>
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> BBa_B0020: 93.5 ng/uL, 260/280 = 1.84
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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> BBa_ B0012: 60.0 ng/uL, 260/280 = 1.93
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Latest revision as of 16:48, 25 September 2012







Bacillus 168 strain provided by Jan-Willem Veening. Grown overnight on LB agar plate at 37C.

Plasmid isolation of bacillus backbone for chromosomal integration (BBa_K090403, size: 5632 bp) and terminator biobricks (BBa_B0020 and BBa_B0012, both size: 2058 bp) using the Roche High Pure Plasmid Isolation Kit.

Plasmid concentrations after isolation:
> BBa_K090403: 50.8 ng/uL, 260/280 = 1.85
> BBa_B0020: 93.5 ng/uL, 260/280 = 1.84
> BBa_ B0012: 60.0 ng/uL, 260/280 = 1.93

Back to notebook



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