Team:METU

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== '''2. Human Voice Induced Gene Circuit Using Electrically Induced Promoters:''' ==
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In this project, our aim is to use human voice as a signal, and stimulate
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a biological system in bacteria by this signal. For this purpose, first
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human voice is converted to electrical signals by an designed electronic
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device. In the second part of the project electrical signals coming from
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electronical device stimulate a promoter and induce a gene expression.
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According to frequency of voice, different currents are created by device,
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and we will try to activate different systems in bacteria by different
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currents.
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To see whether this system will work or not, we are planning to use marker
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genes (such as GFP, RFP or luciferase) as activated by electric stimulus.
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If we succeed to run this system, the future perspectives are to put more
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complicated gene circuits under this mechanism.
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We will use Bacillus Subtilis 168 strain for this project since it is more
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resistant to environmental stress than E.coli.
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|[[Image:METU_logo.png|200px|right|frame]]
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|[[Image:METU_team.png|right|frame|Your team picture]]
 
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|align="center"|[[Team:METU | Team METU]]
 
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<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:METU|Home]]
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!align="center"|[[Team:METU/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=METU Official Team Profile]
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!align="center"|[[Team:METU/Project|Project]]
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<h1 id="logo" class="notext"><a href="https://2012.igem.org/Team:METU">
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!align="center"|[[Team:METU/Parts|Parts Submitted to the Registry]]
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<img width="200px" height="104px" src="/wiki/images/2/22/METU_logo.png" style="padding-top: 10px;">
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!align="center"|[[Team:METU/Modeling|Modeling]]
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!align="center"|[[Team:METU/Notebook|Notebook]]
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!align="center"|[[Team:METU/Safety|Safety]]
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!align="center"|[[Team:METU/Attributions|Attributions]]
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    <h2>Who are we?</h2>
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    <p>We are iGEM 2012 team of METU from Ankara, Turkey. We think iGEM is a great opportunity for crosslinking the fun and science. Therefore, each member of our team had not only highly occupied but also enjoyable summer. For more information about our project visit our wiki page.</p>
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<p>Have fun :)) </p>
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    <h2>CO Converter</h2>
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    <p>Main aim of our project is to convert CO to CO2 and in order to do that we choose to express the enzyme called CODH (Carbon Monoxide Dehydrogenase) which can do this conversion biologically and we wanted to use the enzyme present in <i>Mycobacterium Bovis</i>.</p>
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    <h2>Kill Switch</h2>
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    <p>We needed a kill switch system to keep our genetically modified bacteria under control which are found in CO filter. With the help of this system these modified bacteria would die in the case of its release to the environment.</p>
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    <h2>Cell Limiter</h2>
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    <p>In order to make our biological CO filter’s activity well characterized we designed a quorum sensing system. This system keeps the cell growth under control with the help of quorum sensing signals that detect the cell density and stop the cell division.</p>
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Latest revision as of 22:37, 26 September 2012

  • Who are we?

    We are iGEM 2012 team of METU from Ankara, Turkey. We think iGEM is a great opportunity for crosslinking the fun and science. Therefore, each member of our team had not only highly occupied but also enjoyable summer. For more information about our project visit our wiki page.

    Have fun :))

  • CO Converter

    Main aim of our project is to convert CO to CO2 and in order to do that we choose to express the enzyme called CODH (Carbon Monoxide Dehydrogenase) which can do this conversion biologically and we wanted to use the enzyme present in Mycobacterium Bovis.

  • Kill Switch

    We needed a kill switch system to keep our genetically modified bacteria under control which are found in CO filter. With the help of this system these modified bacteria would die in the case of its release to the environment.

  • Cell Limiter

    In order to make our biological CO filter’s activity well characterized we designed a quorum sensing system. This system keeps the cell growth under control with the help of quorum sensing signals that detect the cell density and stop the cell division.

 
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Sponsors

 

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