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Team:Groningen/Data page
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| - | <li><img src=https://static.igem.org/mediawiki/2012/d/d2/Groningen2012_AD_Hartje.png width=25><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818000" target=_blank>BBa_K818000</a> - <b>Integration vector</b> | + | <li><img src=https://static.igem.org/mediawiki/2012/d/d2/Groningen2012_AD_Hartje.png width=25><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818000" target=_blank>BBa_K818000</a> - <b>Integration vector</b>: integrates into the chromosome of <i>Bacillus subtilis</i> at the <i>SacA</i> locus and replicates in <i>Escherichia coli</i>; derived from vector psac-cm (<a href=http://www.sciencedirect.com/science/article/pii/S0147619X04000332 target =_blank>Middleton et al., 2004</a>).<br><br></li> |
| - | <li><img src=https://static.igem.org/mediawiki/2012/d/d2/Groningen2012_AD_Hartje.png width=25><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818600" target=_blank>BBa_K818600</a> <b>Rotting meat sensor system</b>: | + | <li><img src=https://static.igem.org/mediawiki/2012/d/d2/Groningen2012_AD_Hartje.png width=25><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818600" target=_blank>BBa_K818600</a> <b>Rotting meat sensor system</b>: contains the native <i>Bacillus subtilis</i> promoter P<i>sboA</i> (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818100" target=_blank>BBa_K818100</a>) and yellow chromoprotein amilGFP (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K592010" target=_blank>BBa_K592010</a>) with RBS (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034" target=_blank>BBa_B0034</a>); P<i>sboA</i> is strongly activated by volatiles from rotten meat when <i>B. subtilis</i> grows exponentially. Produces a visible yellow color in six hours.<br><br></li> |
</ol> | </ol> | ||
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<p><b>Data For Pre-existing Parts</b></p> | <p><b>Data For Pre-existing Parts</b></p> | ||
<ol> | <ol> | ||
| - | <li><a href="http://partsregistry.org/ | + | <li><a href="http://partsregistry.org/Part:BBa_K592010:Experience" target=_blank>BBa_K592010</a> <b>Yellow chromoprotein amilGFP </b> (<a href="https://2011.igem.org/Team:Uppsala-Sweden" target=_blank>Uppsala 2011</a>): stable, non-toxic pigment, well perceptible in the visible and fluorescent spectrums. We are the first to characterize the working of the part in <i>Bacillus subtilis</i>.<br><br></li> |
| - | <li><a href="http://partsregistry.org/ | + | <li><a href="http://partsregistry.org/Part:BBa_K592009:Experience" target=_blank>BBa_K592009</a> <b>Blue chromoprotein amilCP </b> (<a href="https://2011.igem.org/Team:Uppsala-Sweden" target=_blank>Uppsala 2011</a>): pigment visible by eye. We are the first to characterize the working of the part in <i>Bacillus subtilis</i>.<br><br></li> |
| - | <li><a href="http://partsregistry.org/ | + | <li><a href="http://partsregistry.org/Part:BBa_K823023:Experience" target=_blank>BBa_K823023</a> <b>Integration vector</b> (<a href="https://2012.igem.org/Team:LMU-Munich" target=_blank>LMU Munich 2012</a>): integrates into the <i>Bacillus subtilis</i> chromosome at the <i>AmyE</i> locus and replicates in <i>E. coli</i>. We confirmed that it works.<br><br></li> |
</ol> | </ol> | ||
<br> | <br> | ||
<p><b>We've Also Characterized the Following Parts</b></p> | <p><b>We've Also Characterized the Following Parts</b></p> | ||
<ol> | <ol> | ||
| - | <li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818100" target=_blank>BBa_K818100</a> <b>Promoter P<i>sboA</i>, rotting meat sensor</b>: | + | <li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818100" target=_blank>BBa_K818100</a> <b>Promoter P<i>sboA</i>, rotting meat sensor</b>: natively activates production of the antibiotic compound biosubtilosin; weakly constitutive expressed at exponential growth, strongly upregulated when subjected to volatiles from spoiled meat. Identified by transcriptome analysis of <i>B. subtilis</i> subjected to volatiles from spoiled meat.<br><br></li> |
| - | <li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818400" target=_blank>BBa_K818400</a> <b>Rotting meat sensor system</b>:promoter P<i>sboA</i> (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818100" target=_blank>BBa_K818100</a>) and | + | <li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818400" target=_blank>BBa_K818400</a> <b>Rotting meat sensor system</b>: promoter P<i>sboA</i> (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818100" target=_blank>BBa_K818100</a>) and blue chromoprotein amilCP (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K592009" target=_blank>BBa_K592009</a>) with RBS (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034" target=_blank>BBa_B0034</a>). Visible blue/purple color is obtained when using our <a href=https://2012.igem.org/Team:Groningen/Sticker target =_blank>sticker system</a>.<br><br></li> |
<br><br><br><br><br> | <br><br><br><br><br> | ||
</ol> | </ol> | ||
Latest revision as of 03:13, 27 October 2012
Data For Our Favorite New Parts
BBa_K818000 - Integration vector: integrates into the chromosome of Bacillus subtilis at the SacA locus and replicates in Escherichia coli; derived from vector psac-cm (Middleton et al., 2004).- BBa_K818600 Rotting meat sensor system: contains the native Bacillus subtilis promoter PsboA (BBa_K818100) and yellow chromoprotein amilGFP (BBa_K592010) with RBS (BBa_B0034); PsboA is strongly activated by volatiles from rotten meat when B. subtilis grows exponentially. Produces a visible yellow color in six hours.
Data For Pre-existing Parts
- BBa_K592010 Yellow chromoprotein amilGFP (Uppsala 2011): stable, non-toxic pigment, well perceptible in the visible and fluorescent spectrums. We are the first to characterize the working of the part in Bacillus subtilis.
- BBa_K592009 Blue chromoprotein amilCP (Uppsala 2011): pigment visible by eye. We are the first to characterize the working of the part in Bacillus subtilis.
- BBa_K823023 Integration vector (LMU Munich 2012): integrates into the Bacillus subtilis chromosome at the AmyE locus and replicates in E. coli. We confirmed that it works.
We've Also Characterized the Following Parts
- BBa_K818100 Promoter PsboA, rotting meat sensor: natively activates production of the antibiotic compound biosubtilosin; weakly constitutive expressed at exponential growth, strongly upregulated when subjected to volatiles from spoiled meat. Identified by transcriptome analysis of B. subtilis subjected to volatiles from spoiled meat.
- BBa_K818400 Rotting meat sensor system: promoter PsboA (BBa_K818100) and blue chromoprotein amilCP (BBa_K592009) with RBS (BBa_B0034). Visible blue/purple color is obtained when using our sticker system.
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