Team:Groningen/Notebook/Wetwork 27June2012

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Tom
 
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Plasmid isolation of LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) biobricks.
 
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Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul.
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'''MicroArray experiment: preparations'''
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Tom <br> <br>
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Arjan/Renske
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Plasmid isolation of LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) biobricks. <br> <br>
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Made setup for the micro-array experiment:
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Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul. <br><br>
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37 degrees room. Flask with 50 mL culture of Bacillus subtilis sp. 168, connected with tubes to 100 mL flask with rotting/fresh meat. Filters and peristaltic pump inbetween. Culture is stirred by a magnetic stirrer.
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[[File:Groningen_RR_20120628_microarraysetup.jpg|thumb|400px|left|Micro-Array Setup]]
 
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Emeraldo/Nisa
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Emeraldo/Nisa <br>
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<br>
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1.) Order SpeI and EcoRI FastDigest Fermentas<br>
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2.) Order Plamid High Pure purification kit Roche<br>
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3.) B. subtilis transformation with BBa_K090403 <br>
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4.) Digestion of pigment genes from their backbones with EcoRI and SpeI. Result: the pigments genes are in correct length. <br>
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5.) Digestion of BBa_K090403. Result: incorrect length.<br><br>
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1.) Order SpeI and EcoRI FastDigest Fermentas
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2.) Order Plamid High Pure purification kit Roche
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MicroArray experiment: preparations <br><br>
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3.) B. subtilis transformation with BBa_K090403
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4.) Digestion of pigment genes from their backbones with EcoRI and SpeI. Result: the pigments genes are in correct length.
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Arjan/Renske <br><br>
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5.) Digestion of BBa_K090403. Result: incorrect length.
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Made setup for the micro-array experiment: <br>
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37 degrees room. Flask with 50 mL culture <br>
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of Bacillus subtilis sp. 168,<br>
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connected with tubes to 100 mL flask <br>
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with rotting/fresh meat. <br>
 +
Filters and peristaltic pump inbetween. <br>
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Culture is stirred by a magnetic stirrer.<br><br>
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<img src="https://static.igem.org/mediawiki/2012/9/9e/Groningen2012_RR_MicArraysetup.png" width="300" height="300">
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<br>
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Latest revision as of 16:52, 25 September 2012








Tom

Plasmid isolation of LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) biobricks.

Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul.

Emeraldo/Nisa

1.) Order SpeI and EcoRI FastDigest Fermentas
2.) Order Plamid High Pure purification kit Roche
3.) B. subtilis transformation with BBa_K090403
4.) Digestion of pigment genes from their backbones with EcoRI and SpeI. Result: the pigments genes are in correct length.
5.) Digestion of BBa_K090403. Result: incorrect length.

MicroArray experiment: preparations

Arjan/Renske

Made setup for the micro-array experiment:
37 degrees room. Flask with 50 mL culture
of Bacillus subtilis sp. 168,
connected with tubes to 100 mL flask
with rotting/fresh meat.
Filters and peristaltic pump inbetween.
Culture is stirred by a magnetic stirrer.


Back to notebook



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