Team:OUC-China

From 2012.igem.org

(Difference between revisions)
 
(86 intermediate revisions not shown)
Line 1: Line 1:
<html>
<html>
-
<head>
 
<meta charset="UTF-8">
<meta charset="UTF-8">
<meta name="viewport" content="width=device-width initial-scale=1"/>
<meta name="viewport" content="width=device-width initial-scale=1"/>
Line 16: Line 15:
<script type="text/javascript" src="http://1.oucigem.sinaapp.com/jquery.cslider.js"></script><!--slideshow jquery js-->
<script type="text/javascript" src="http://1.oucigem.sinaapp.com/jquery.cslider.js"></script><!--slideshow jquery js-->
<script type="text/javascript" src="http://1.oucigem.sinaapp.com/modernizr.custom.28468.js"></script><!--slideshow main js-->
<script type="text/javascript" src="http://1.oucigem.sinaapp.com/modernizr.custom.28468.js"></script><!--slideshow main js-->
 +
<div>
 +
        <script type="text/javascript" src="http://1.oucigem.sinaapp.com/ouc-home-hof.js"></script><!--hof js-->
 +
        <link href='http://1.oucigem.sinaapp.com/ouc-home-hof.css' rel='stylesheet' type='text/css'>
 +
</div>
<style>
<style>
h3 {
h3 {
-
font-size: 20px;
+
font-size: 27px;
 +
}
 +
#top-section{
 +
  width:99%;
 +
}
 +
#menubar{
 +
      top:0;
 +
}
 +
a:hover {
 +
    text-decoration: none;
 +
}
 +
#footer-box{
 +
height: 50px;
 +
width:100%;
 +
margin-top:10px;
 +
margin-bottom:-10px;
 +
background-color:#1487DF;
 +
border:none;
 +
padding-left:0;
 +
padding-right:0;
 +
}
 +
#ouc-footer{
 +
      text-align: center;
 +
      margin-top: 100px;
 +
}
 +
#footer a
 +
{
 +
color:#ffffff;
 +
        font-size:16px;
 +
        line-height: 22px;
 +
}
 +
#ouc-tools ul li {
 +
    display: inline-block;
 +
    margin-right: 20px;
 +
    vertical-align: middle;
 +
}
 +
.nr {
 +
padding-left: 240px;
 +
}
 +
.hmoverview{
 +
height:394px;
 +
background: url(https://static.igem.org/mediawiki/2012/e/ea/Ouc-home-humanpractice.jpg) 50% 0 no-repeat;
 +
margin: 20px auto 0;
 +
}
 +
.hmguide{
 +
width: 1000px;
 +
height: 430px;
 +
margin: 0 auto;
 +
padding: 0 10px;
 +
}
 +
.hmguide h3{
 +
line-height:50px;
 +
}
 +
.hmguide ul{
 +
        list-style:none;
 +
}
 +
.hmguide ul li{
 +
width: 250px;
 +
height: 365px;
 +
float: left;
 +
margin-left: 70px;
 +
}
 +
.hmguide ul li img{
 +
width: 240px;
 +
height: 180px;
 +
border: 5px solid #f3f3f3;
 +
}
 +
.hmguide ul li h4{
 +
width: 250px;
 +
line-height: 16px;
 +
font-size: 16px;
 +
margin-top:5px;
 +
text-align: center;
 +
height:32px;
 +
color:black;
 +
}
 +
.hmguide ul li h4 a{
 +
color:#000;
 +
}
 +
.hmguide ul li h4 a:hover{
 +
color:#168eda;
 +
}
 +
.hmguide ul li p span{
 +
        color:#0d7ad5;
 +
float: right;
 +
        font-weight:bold;
 +
}
 +
.hmguide ul li p span a{
 +
        color:#0d7ad5;
 +
}
 +
.hmguide ul li p span a:visited{
 +
color:#168eda;
}
}
</style>
</style>
-
</head>
+
 
-
<body>
+
<div id="menu-wrapper">
<div id="menu-wrapper">
<div id="logo">
<div id="logo">
</div>
</div>
<ul class="menu">
<ul class="menu">
-
<li> <a href="https://2012.igem.org/Team:OUC-China">Home</a> </li>
+
<li> <a href="#">Index</a> </li>
<li> <a href="https://2012.igem.org/Team:OUC-China/Project/Overview">Project</a> </li>
<li> <a href="https://2012.igem.org/Team:OUC-China/Project/Overview">Project</a> </li>
<li> <a href="https://2012.igem.org/Team:OUC-China/Modeling/Overview">Modeling</a> </li>
<li> <a href="https://2012.igem.org/Team:OUC-China/Modeling/Overview">Modeling</a> </li>
Line 40: Line 133:
<ul class="submenu">
<ul class="submenu">
<li>
<li>
-
<a href="https://2012.igem.org/Team:OUC-China/Project/Overview">
+
<a href="https://2012.igem.org/Team:OUC-China">
<img src="https://static.igem.org/mediawiki/2012/a/af/Projectoverview.jpg" />
<img src="https://static.igem.org/mediawiki/2012/a/af/Projectoverview.jpg" />
-
Project Overview
+
Home
</a>
</a>
</li>
</li>
<li>
<li>
-
<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Overview">
+
<a href="https://2012.igem.org/Team:OUC-China#hmo">
<img src="https://static.igem.org/mediawiki/2012/4/49/Humanpracticeoverview.jpg" />
<img src="https://static.igem.org/mediawiki/2012/4/49/Humanpracticeoverview.jpg" />
-
HumanPractice Overview
+
HumanPractice index
</a>
</a>
</li>
</li>
Line 60: Line 153:
<a href="https://2012.igem.org/Team:OUC-China/contactus">
<a href="https://2012.igem.org/Team:OUC-China/contactus">
<img src="https://static.igem.org/mediawiki/2012/1/1e/Contact-us.jpg" />
<img src="https://static.igem.org/mediawiki/2012/1/1e/Contact-us.jpg" />
 +
Contact Us
</a>
</a>
-
Contact Us
+
</li>
</li>
</ul>
</ul>
Line 158: Line 252:
<a href="https://2012.igem.org/Team:OUC-China/Team/AcknowledgementandCooperation">
<a href="https://2012.igem.org/Team:OUC-China/Team/AcknowledgementandCooperation">
<img src="https://static.igem.org/mediawiki/2012/a/a6/Ouc-nav-co.jpg" />
<img src="https://static.igem.org/mediawiki/2012/a/a6/Ouc-nav-co.jpg" />
-
Acknowledgement and Cooperation
+
Acknowledgement&Cooperation
</a>
</a>
</li>
</li>
Line 200: Line 294:
<a href="https://2012.igem.org/Team:OUC-China/protocol">
<a href="https://2012.igem.org/Team:OUC-China/protocol">
<img src="https://static.igem.org/mediawiki/2012/7/7d/Ouc-pro.png" />
<img src="https://static.igem.org/mediawiki/2012/7/7d/Ouc-pro.png" />
-
ProtocolS
+
Protocols
</a>
</a>
</li>
</li>
Line 209: Line 303:
<div class="da-slide">
<div class="da-slide">
<h2 >Overview</h2>
<h2 >Overview</h2>
-
<p>The sensors for next-generation detector of red tide!</p>
+
<p>Red tide detector:
-
<a href="" class="da-link">Read more</a>
+
Facilitate small RNA as information processing media;
-
<div class="da-img"><img src="" alt="senso" /></div>
+
<br/>
 +
and gas vesicle as a novel output actuator...
 +
<br/>
 +
Response to the overloading phosphate or nitrate when they exceeds warning limit...<br/>
 +
</p>
 +
<a href="https://2012.igem.org/Team:OUC-China/Project/Overview" class="da-link">Read more</a>
 +
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/1/19/Ouc-final.png" alt="overview" /></div>
</div>
</div>
<div class="da-slide">
<div class="da-slide">
Line 222: Line 322:
<h2>Model-Driven Design</h2>
<h2>Model-Driven Design</h2>
<p>
<p>
-
  Parameter optimization in ternary system...
+
  Get specific directions for ternary system design from simulation...
</p>
</p>
<a href="https://2012.igem.org/Team:OUC-China/Modeling/Overview" class="da-link">Read more</a>
<a href="https://2012.igem.org/Team:OUC-China/Modeling/Overview" class="da-link">Read more</a>
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/2/26/Home-modeling.png" alt="Home-modeling" /></div>
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/2/26/Home-modeling.png" alt="Home-modeling" /></div>
</div>
</div>
-
<div class="da-slide">
+
<div class="da-slide">
<h2>Making decision through sRNA</h2>
<h2>Making decision through sRNA</h2>
<p>The sophisticated  'CPU' of our red tide detector...<br/>
<p>The sophisticated  'CPU' of our red tide detector...<br/>
Line 233: Line 333:
<a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract" class="da-link">Read more</a>
<a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract" class="da-link">Read more</a>
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/2/2c/Home-decision-making-device.png" alt="Home-decision-making-device" /></div>
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/2/2c/Home-decision-making-device.png" alt="Home-decision-making-device" /></div>
 +
</div>
 +
 +
<div class="da-slide">
 +
<h2>Floating Device</h2>
 +
<p>The output actuator of our red tide detector...<br/>
 +
Gas vesicle as our floating device</p>
 +
<a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground" class="da-link">Read more</a>
 +
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/a/a2/Ouc-home-floating-device.png" /></div>
</div>
</div>
<nav class="da-arrows">
<nav class="da-arrows">
Line 242: Line 350:
<div id="project_intro_box">
<div id="project_intro_box">
<div class="project_intro">
<div class="project_intro">
-
<h2 style="border-bottom:none;">Oceanfloat and Oceanfeel<br/><span>----a floating biosenser</span></h2>
+
<h2>Oceanfloat and Oceanfeel<br/><span>——a floating biosensor</span></h2>
</div>
</div>
</div>
</div>
Line 250: Line 358:
<div>
<div>
<ul id="tab">
<ul id="tab">
-
<li class="on" id="tab_1" onclick="switchTab(1)"><img src="https://static.igem.org/mediawiki/2012/8/8f/Ouc-home-step1.png" /><span>step 1</span></li>
+
<li class="on" id="tab_1" onclick="switchTab(1)"><img src="https://static.igem.org/mediawiki/2012/8/8f/Ouc-home-step1.png" /><span>Part1</span></li>
-
<li id="tab_2" onclick="switchTab(2)"><img src="https://static.igem.org/mediawiki/2012/d/de/Ouc-home-step3.png" /><span>step 2</span></li>
+
<li id="tab_2" onclick="switchTab(2)"><img src="https://static.igem.org/mediawiki/2012/d/de/Ouc-home-step3.png" /><span>Part2</span></li>
-
<li id="tab_3" onclick="switchTab(3)"><img src="https://static.igem.org/mediawiki/2012/a/a4/Ouc-home-steplast.png" /><span>step 3</span></li>
+
<li id="tab_3" onclick="switchTab(3)"><img src="https://static.igem.org/mediawiki/2012/a/a4/Ouc-home-steplast.png" /><span>Part3</span></li>
</ul>
</ul>
<div style="clear: both;">&nbsp;</div>
<div style="clear: both;">&nbsp;</div>
Line 260: Line 368:
<div class="tup"><img src="https://static.igem.org/mediawiki/2012/3/3a/Ouc-home-step1-2.png" /></div>
<div class="tup"><img src="https://static.igem.org/mediawiki/2012/3/3a/Ouc-home-step1-2.png" /></div>
<div class="nr">
<div class="nr">
-
<h1>Step 1</h1>
+
<h1>Sensor</h1>
<p>Our phosphate- and nitrate-sensitive devices are responsible for detection of environmental phosphate and nitrate. Besides that they transform the input signals into "readable information" for decision-making devices.</p>
<p>Our phosphate- and nitrate-sensitive devices are responsible for detection of environmental phosphate and nitrate. Besides that they transform the input signals into "readable information" for decision-making devices.</p>
<p>much work has been done for characterization and modification of nitrate- and phosphate-sensitive promoters. A whole set of design ideas for TCS modifications are also presented for other igemers. Additionally an actuator that can remove the phosphate is recommended.</p>
<p>much work has been done for characterization and modification of nitrate- and phosphate-sensitive promoters. A whole set of design ideas for TCS modifications are also presented for other igemers. Additionally an actuator that can remove the phosphate is recommended.</p>
Line 270: Line 378:
<div class="tup"><img src="https://static.igem.org/mediawiki/2012/a/a1/Ouc-home-step3-02.png" /></div>
<div class="tup"><img src="https://static.igem.org/mediawiki/2012/a/a1/Ouc-home-step3-02.png" /></div>
<div class="nr">
<div class="nr">
-
<h1>Step2</h1>
+
<h1>Decision-making Device</h1>
<p>Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.</p>
<p>Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.</p>
<p>Both comparator and ratio sensor facilitate a ternary system consisted of two small RNA and GFP mRNA. The concentration change of external two inducers leads to the transcription rate change of small RNA(alpha s)in this ternary system, the interactions between three RNAs will lead to interesting behaviors of our system like sensing the difference between two inputs quantitatively(Comparator) or the ratio of two inputs(Ratio senor).</p>
<p>Both comparator and ratio sensor facilitate a ternary system consisted of two small RNA and GFP mRNA. The concentration change of external two inducers leads to the transcription rate change of small RNA(alpha s)in this ternary system, the interactions between three RNAs will lead to interesting behaviors of our system like sensing the difference between two inputs quantitatively(Comparator) or the ratio of two inputs(Ratio senor).</p>
Line 280: Line 388:
<div class="tup"><img src="https://static.igem.org/mediawiki/2012/f/fa/Ouc-step3-last.png" /></div>
<div class="tup"><img src="https://static.igem.org/mediawiki/2012/f/fa/Ouc-step3-last.png" /></div>
<div class="nr">
<div class="nr">
-
<h1>Step3</h1>
+
<h1>Floating Device</h1>
-
<p>In our project, by transforming the buoyancy device BBa_K737010, the gas vesicle cluster from the genome of Planktothrix rubescens into E.Coli, we succeed in making Escherichia.Coli floating on the surface of the water. It can help the cells produce the gas vesicle inside, which could make densities significant stratification in the culture. Not only the gene cluster but the two parts BBa_K737006 and BBa_K737007, which produce the gas vesicle structure protein, can also make the Escherichia.Coli floating. We use the shorter length of the part achieve a better effect than BBa_K190033 and the gas vesicle application prospect.</p>
+
<p>In our project, by transforming the buoyancy device BBa_K737010(designed by ourselves), the gas vesicle cluster from the genome of Planktothrix rubescens into E.Coli, we succeed in making Escherichia.Coli floating on the surface of the water. It can help the cells produce the gas vesicle inside, which could make densities significant stratification in the culture. Not only the gene cluster but the two parts BBa_K737006 and BBa_K737007, which produce the gas vesicle structure protein, can also make the Escherichia.Coli floating. We use the shorter length of the part achieve a better effect than BBa_K190033 and the gas vesicle application prospect.</p>
<p><a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground"><strong>learn more</strong></a>
<p><a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground"><strong>learn more</strong></a>
</div>
</div>
Line 289: Line 397:
</div>
</div>
</section>
</section>
-
<br/>
+
<div class="hmoverview" id="hmo"></div>
-
<br/>
+
<div class="hmguide">
-
<br/>
+
<h3>Human practice</h3>
-
<br/>
+
<ul>
-
<br/>
+
<li>
-
<br/>
+
<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication" title="Meeting and Academic Communication" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/d7/Ouc-homehp01.jpg" alt="Meeting and Academic Communication" title="Meeting and Academic Communication" /></a>
-
<br/>
+
<h4><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication" title="Meeting and Academic Communication" target="_blank">Meeting and Academic Communication</a></h4>
 +
<p>As a new born iGEM team,questions below puzzled us for a very long time. However,Peking Univerisity iGEM team helped us a lot by giving us dire……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication" title="Meeting and Academic Communication" target="_blank">[Learn more]</a></span></p>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps" title="Camps, Class and Lectures" target="_blank"><img src="https://static.igem.org/mediawiki/2012/a/a5/Ouc-homehp02.jpg" alt="Camps, Class and Lectures" title="Camps, Class and Lectures" /></a>
 +
<h4><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps" title="Camps, Class and Lectures" target="_blank">Camps, Class and Lectures</a></h4>
 +
<p>Last year, we held 1st summer camp of life science and technology. Through this camp, most of the participants……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps" title="Camps, Class and Lectures" target="_blank">[Learn more]</a></span></p>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM" title="Special OUC-China HP" target="_blank"><img src="https://static.igem.org/mediawiki/2012/4/40/Ouc-homehp03.jpg" alt="Special OUC-China HP" title="Special OUC-China HP" /></a>
 +
<h4><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM" title="Special OUC-China HP" target="_blank">Special OUC-China HP</a></h4>
 +
<p>Because our project of this focuses on the ratio of N and P and cyanobacteria in the sea water, we consulted professors who study on ecology……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM" title="Special OUC-China HP" target="_blank">[Learn more]</a></span></p>
 +
</li>
 +
</ul>
 +
</div>
 +
<br/>
 +
<div id="ouc-footer">
 +
        <div id="ouc-tools">
 +
         
 +
            <ul>
 +
                <li id="ouc-clustrmaps-wrapper">
 +
                    <div id="clustrmaps-widget"></div><script type="text/javascript">var _clustrmaps = {'url' : 'https://2012.igem.org/Team:OUC-China', 'user' : 1034709, 'server' : '4', 'id' : 'clustrmaps-widget', 'version' : 1, 'date' : '2012-09-22', 'lang' : 'zh', 'corners' : 'square' };(function (){ var s = document.createElement('script'); s.type = 'text/javascript'; s.async = true; s.src = 'http://www4.clustrmaps.com/counter/map.js'; var x = document.getElementsByTagName('script')[0]; x.parentNode.insertBefore(s, x);})();</script><noscript><a href="http://www4.clustrmaps.com/user/c56fc9d5"><img src="http://www4.clustrmaps.com/stats/maps-no_clusters/2012.igem.org-Team-OUC-China-thumb.jpg" alt="Locations of visitors to this page" /></a></noscript>
 +
                </li>
 +
                <li>
 +
<a href="http://www.renren.com/296396903/profile?ref=opensearch_normal"><img src="https://static.igem.org/mediawiki/2012/d/d2/Ouc-footer-cmlsast.jpg" alt="OUC CMLS AST" border="0"></a> 
 +
                </li>
 +
                <li>
 +
                    <a href="https://2012.igem.org/Team:OUC-China/contactus"><img src="https://static.igem.org/mediawiki/2012/5/5e/OUC-footer-contact.jpg" alt="Contact Us" border="0"></a>
 +
                </li>
 +
                <li style="width:160px;">
 +
<form action="/Special:Search" id="searchform" style=" border-color:#000;">
 +
<input id="searchInput" name="search" type="text" title="Search 2011.igem.org [f]" accesskey="f" value="">
 +
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;
 +
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" />
 +
</form>
 +
                </li>
 +
            </ul>
 +
        </div>
 +
     
 +
    </div>
 +
 
<script type="text/javascript">
<script type="text/javascript">
$(function() {
$(function() {
Line 319: Line 467:
<script>
<script>
function goto(id) {
function goto(id) {
-
         $("#" + id).ScrollTo(800);
+
         $("#" + id).ScrollTo(750);
}
}
function switchTab(n){
function switchTab(n){
Line 334: Line 482:
}
}
</script>
</script>
-
</body>
 
</html>
</html>

Latest revision as of 13:25, 26 October 2012

Overview

Red tide detector: Facilitate small RNA as information processing media;
and gas vesicle as a novel output actuator...
Response to the overloading phosphate or nitrate when they exceeds warning limit...

Read more
overview

Sensor

The sensors for next-generation detector of red tide!

Read more
sensor

Model-Driven Design

Get specific directions for ternary system design from simulation...

Read more
Home-modeling

Making decision through sRNA

The sophisticated 'CPU' of our red tide detector...
Engineering CPU for your own "synthia"!

Read more
Home-decision-making-device

Floating Device

The output actuator of our red tide detector...
Gas vesicle as our floating device

Read more

Oceanfloat and Oceanfeel
——a floating biosensor

Our Project

  • Part1
  • Part2
  • Part3
 
  • Sensor

    Our phosphate- and nitrate-sensitive devices are responsible for detection of environmental phosphate and nitrate. Besides that they transform the input signals into "readable information" for decision-making devices.

    much work has been done for characterization and modification of nitrate- and phosphate-sensitive promoters. A whole set of design ideas for TCS modifications are also presented for other igemers. Additionally an actuator that can remove the phosphate is recommended.

    learn more

     
  • Decision-making Device

    Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.

    Both comparator and ratio sensor facilitate a ternary system consisted of two small RNA and GFP mRNA. The concentration change of external two inducers leads to the transcription rate change of small RNA(alpha s)in this ternary system, the interactions between three RNAs will lead to interesting behaviors of our system like sensing the difference between two inputs quantitatively(Comparator) or the ratio of two inputs(Ratio senor).

    learn more

     
  • Floating Device

    In our project, by transforming the buoyancy device BBa_K737010(designed by ourselves), the gas vesicle cluster from the genome of Planktothrix rubescens into E.Coli, we succeed in making Escherichia.Coli floating on the surface of the water. It can help the cells produce the gas vesicle inside, which could make densities significant stratification in the culture. Not only the gene cluster but the two parts BBa_K737006 and BBa_K737007, which produce the gas vesicle structure protein, can also make the Escherichia.Coli floating. We use the shorter length of the part achieve a better effect than BBa_K190033 and the gas vesicle application prospect.

    learn more

     

Human practice