Team:OUC-China

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<h2>Overview</h2>
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<h2 >Overview</h2>
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<p>The sensors for next-generation detector of red tide!</p>
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<p>Red tide detector:
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<a href="" class="da-link">Read more</a>
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Facilitate small RNA as information processing media;
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and gas vesicle as a novel output actuator...
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<br/>
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Response to the overloading phosphate or nitrate when they exceeds warning limit...<br/>
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</p>
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<a href="https://2012.igem.org/Team:OUC-China/Project/Overview" class="da-link">Read more</a>
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<h2>Model-Driven Design</h2>
<h2>Model-Driven Design</h2>
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  Parameter optimization in ternary system...
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  Get specific directions for ternary system design from simulation...
</p>
</p>
<a href="https://2012.igem.org/Team:OUC-China/Modeling/Overview" class="da-link">Read more</a>
<a href="https://2012.igem.org/Team:OUC-China/Modeling/Overview" class="da-link">Read more</a>
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/2/26/Home-modeling.png" alt="Home-modeling" /></div>
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/2/26/Home-modeling.png" alt="Home-modeling" /></div>
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<h2>Making decision through sRNA...</h2>
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<h2>Making decision through sRNA</h2>
<p>The sophisticated  'CPU' of our red tide detector...<br/>
<p>The sophisticated  'CPU' of our red tide detector...<br/>
Engineering CPU for your own "synthia"!</p>
Engineering CPU for your own "synthia"!</p>
<a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract" class="da-link">Read more</a>
<a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract" class="da-link">Read more</a>
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/2/2c/Home-decision-making-device.png" alt="Home-decision-making-device" /></div>
<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/2/2c/Home-decision-making-device.png" alt="Home-decision-making-device" /></div>
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<h2>Floating Device</h2>
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<p>The output actuator of our red tide detector...<br/>
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Gas vesicle as our floating device</p>
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<a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground" class="da-link">Read more</a>
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<div class="da-img"><img src="https://static.igem.org/mediawiki/2012/a/a2/Ouc-home-floating-device.png" /></div>
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<h2>Oceanfloat and Oceanfeel<br/><span>----a floating biosenser</span></h2>
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<h2>Oceanfloat and Oceanfeel<br/><span>——a floating biosensor</span></h2>
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<li class="on" id="tab_1" onclick="switchTab(1)"><img src="https://static.igem.org/mediawiki/2012/8/8f/Ouc-home-step1.png" /><span>step 1</span></li>
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<li class="on" id="tab_1" onclick="switchTab(1)"><img src="https://static.igem.org/mediawiki/2012/8/8f/Ouc-home-step1.png" /><span>Part1</span></li>
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<li id="tab_2" onclick="switchTab(2)"><img src="https://static.igem.org/mediawiki/2012/d/de/Ouc-home-step3.png" /><span>step 2</span></li>
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<li id="tab_2" onclick="switchTab(2)"><img src="https://static.igem.org/mediawiki/2012/d/de/Ouc-home-step3.png" /><span>Part2</span></li>
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<li id="tab_3" onclick="switchTab(3)"><img src="https://static.igem.org/mediawiki/2012/a/a4/Ouc-home-steplast.png" /><span>step 3</span></li>
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<li id="tab_3" onclick="switchTab(3)"><img src="https://static.igem.org/mediawiki/2012/a/a4/Ouc-home-steplast.png" /><span>Part3</span></li>
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<h1>Step 1</h1>
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<h1>Sensor</h1>
<p>Our phosphate- and nitrate-sensitive devices are responsible for detection of environmental phosphate and nitrate. Besides that they transform the input signals into "readable information" for decision-making devices.</p>
<p>Our phosphate- and nitrate-sensitive devices are responsible for detection of environmental phosphate and nitrate. Besides that they transform the input signals into "readable information" for decision-making devices.</p>
<p>much work has been done for characterization and modification of nitrate- and phosphate-sensitive promoters. A whole set of design ideas for TCS modifications are also presented for other igemers. Additionally an actuator that can remove the phosphate is recommended.</p>
<p>much work has been done for characterization and modification of nitrate- and phosphate-sensitive promoters. A whole set of design ideas for TCS modifications are also presented for other igemers. Additionally an actuator that can remove the phosphate is recommended.</p>
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<div class="tup"><img src="https://static.igem.org/mediawiki/2012/a/a1/Ouc-home-step3-02.png" /></div>
<div class="tup"><img src="https://static.igem.org/mediawiki/2012/a/a1/Ouc-home-step3-02.png" /></div>
<div class="nr">
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<h1>Step2</h1>
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<h1>Decision-making Device</h1>
<p>Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.</p>
<p>Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.</p>
<p>Both comparator and ratio sensor facilitate a ternary system consisted of two small RNA and GFP mRNA. The concentration change of external two inducers leads to the transcription rate change of small RNA(alpha s)in this ternary system, the interactions between three RNAs will lead to interesting behaviors of our system like sensing the difference between two inputs quantitatively(Comparator) or the ratio of two inputs(Ratio senor).</p>
<p>Both comparator and ratio sensor facilitate a ternary system consisted of two small RNA and GFP mRNA. The concentration change of external two inducers leads to the transcription rate change of small RNA(alpha s)in this ternary system, the interactions between three RNAs will lead to interesting behaviors of our system like sensing the difference between two inputs quantitatively(Comparator) or the ratio of two inputs(Ratio senor).</p>
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<div class="tup"><img src="https://static.igem.org/mediawiki/2012/f/fa/Ouc-step3-last.png" /></div>
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<h1>Step3</h1>
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<h1>Floating Device</h1>
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<p>In our project, by transforming the buoyancy device BBa_K737010, the gas vesicle cluster from the genome of Planktothrix rubescens into E.Coli, we succeed in making Escherichia.Coli floating on the surface of the water. It can help the cells produce the gas vesicle inside, which could make densities significant stratification in the culture. Not only the gene cluster but the two parts BBa_K737006 and BBa_K737007, which produce the gas vesicle structure protein, can also make the Escherichia.Coli floating. We use the shorter length of the part achieve a better effect than BBa_K190033 and the gas vesicle application prospect.</p>
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<p>In our project, by transforming the buoyancy device BBa_K737010(designed by ourselves), the gas vesicle cluster from the genome of Planktothrix rubescens into E.Coli, we succeed in making Escherichia.Coli floating on the surface of the water. It can help the cells produce the gas vesicle inside, which could make densities significant stratification in the culture. Not only the gene cluster but the two parts BBa_K737006 and BBa_K737007, which produce the gas vesicle structure protein, can also make the Escherichia.Coli floating. We use the shorter length of the part achieve a better effect than BBa_K190033 and the gas vesicle application prospect.</p>
<p><a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground"><strong>learn more</strong></a>
<p><a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground"><strong>learn more</strong></a>
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<h3>Human practice</h3>
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<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication" title="Meeting and Academic Communication" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/d7/Ouc-homehp01.jpg" alt="Meeting and Academic Communication" title="Meeting and Academic Communication" /></a>
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<h4><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication" title="Meeting and Academic Communication" target="_blank">Meeting and Academic Communication</a></h4>
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<p>As a new born iGEM team,questions below puzzled us for a very long time. However,Peking Univerisity iGEM team helped us a lot by giving us dire……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication" title="Meeting and Academic Communication" target="_blank">[Learn more]</a></span></p>
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<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps" title="Camps, Class and Lectures" target="_blank"><img src="https://static.igem.org/mediawiki/2012/a/a5/Ouc-homehp02.jpg" alt="Camps, Class and Lectures" title="Camps, Class and Lectures" /></a>
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<h4><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps" title="Camps, Class and Lectures" target="_blank">Camps, Class and Lectures</a></h4>
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<p>Last year, we held 1st summer camp of life science and technology. Through this camp, most of the participants……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps" title="Camps, Class and Lectures" target="_blank">[Learn more]</a></span></p>
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<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM" title="Special OUC-China HP" target="_blank"><img src="https://static.igem.org/mediawiki/2012/4/40/Ouc-homehp03.jpg" alt="Special OUC-China HP" title="Special OUC-China HP" /></a>
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<h4><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM" title="Special OUC-China HP" target="_blank">Special OUC-China HP</a></h4>
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<p>Because our project of this focuses on the ratio of N and P and cyanobacteria in the sea water, we consulted professors who study on ecology……<span><a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM" title="Special OUC-China HP" target="_blank">[Learn more]</a></span></p>
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Latest revision as of 13:25, 26 October 2012

Overview

Red tide detector: Facilitate small RNA as information processing media;
and gas vesicle as a novel output actuator...
Response to the overloading phosphate or nitrate when they exceeds warning limit...

Read more
overview

Sensor

The sensors for next-generation detector of red tide!

Read more
sensor

Model-Driven Design

Get specific directions for ternary system design from simulation...

Read more
Home-modeling

Making decision through sRNA

The sophisticated 'CPU' of our red tide detector...
Engineering CPU for your own "synthia"!

Read more
Home-decision-making-device

Floating Device

The output actuator of our red tide detector...
Gas vesicle as our floating device

Read more

Oceanfloat and Oceanfeel
——a floating biosensor

Our Project

  • Part1
  • Part2
  • Part3
 

  • Sensor

    Our phosphate- and nitrate-sensitive devices are responsible for detection of environmental phosphate and nitrate. Besides that they transform the input signals into "readable information" for decision-making devices.

    much work has been done for characterization and modification of nitrate- and phosphate-sensitive promoters. A whole set of design ideas for TCS modifications are also presented for other igemers. Additionally an actuator that can remove the phosphate is recommended.

    learn more

     

  • Decision-making Device

    Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.

    Both comparator and ratio sensor facilitate a ternary system consisted of two small RNA and GFP mRNA. The concentration change of external two inducers leads to the transcription rate change of small RNA(alpha s)in this ternary system, the interactions between three RNAs will lead to interesting behaviors of our system like sensing the difference between two inputs quantitatively(Comparator) or the ratio of two inputs(Ratio senor).

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  • Floating Device

    In our project, by transforming the buoyancy device BBa_K737010(designed by ourselves), the gas vesicle cluster from the genome of Planktothrix rubescens into E.Coli, we succeed in making Escherichia.Coli floating on the surface of the water. It can help the cells produce the gas vesicle inside, which could make densities significant stratification in the culture. Not only the gene cluster but the two parts BBa_K737006 and BBa_K737007, which produce the gas vesicle structure protein, can also make the Escherichia.Coli floating. We use the shorter length of the part achieve a better effect than BBa_K190033 and the gas vesicle application prospect.

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Human practice


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