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Team:Groningen/Notebook/Wetwork 15August2012
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| - | Sub-culturing the transformats colonies on LB agar plates | + | <p class="margin"> |
| - | + | Tom <br> | |
| - | Nisa | + | <br> |
| - | Promoters-pigment cloning into pSB1C3. Molarity ratio plasmid:DNA insert = 1:3. | + | Ligation of alsT(150, 250, 300, 500) with GFP and psb1c3 and pfu promotor with GFP and psb1c3. <br> |
| - | Promoter used in this step: fnr and lacI | + | Result: Transformation in E.coli was succesfull. <br> |
| - | Pigment used in this step: lycopene, amilCP, amilGFP | + | <br> |
| - | Ligation was done for 2 hours in room temperature before continue to E. coli transformation. | + | PCR of GFP with preffix and suffix and PCR of GFP that can couple to pfu. <br> |
| - | + | Result: only PCR product: GFP preffix suffix was succesfull.<br> | |
| - | < | + | <br> |
| + | Emeraldo <br> | ||
| + | Sub-culturing the transformats colonies on LB agar plates <br> | ||
| + | <br> | ||
| + | Nisa <br> | ||
| + | Promoters-pigment cloning into pSB1C3. Molarity ratio plasmid:DNA insert = 1:3. <br> | ||
| + | Promoter used in this step: fnr and lacI <br> | ||
| + | Pigment used in this step: lycopene, amilCP, amilGFP <br> | ||
| + | Ligation was done for 2 hours in room temperature before continue to E. coli transformation. <br> | ||
| + | <br> | ||
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
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Latest revision as of 13:32, 26 September 2012
Tom
Ligation of alsT(150, 250, 300, 500) with GFP and psb1c3 and pfu promotor with GFP and psb1c3.
Result: Transformation in E.coli was succesfull.
PCR of GFP with preffix and suffix and PCR of GFP that can couple to pfu.
Result: only PCR product: GFP preffix suffix was succesfull.
Emeraldo
Sub-culturing the transformats colonies on LB agar plates
Nisa
Promoters-pigment cloning into pSB1C3. Molarity ratio plasmid:DNA insert = 1:3.
Promoter used in this step: fnr and lacI
Pigment used in this step: lycopene, amilCP, amilGFP
Ligation was done for 2 hours in room temperature before continue to E. coli transformation.
Back to notebook
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