Team:Groningen/Notebook/Wetwork 14August2012

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Ligation of Fnr promotor + GFP in psb1c3. Ratio of ligation 1:1:1 and 2:1:1 Fnr:GFP:psb1c3.
 
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Result: Transformation in E.coli did not work.
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Nisa
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alsT promoter PCR -->OK! Proceeding to ligation and transformation tomorrow.
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Tom<br>
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Ligation of Fnr promotor + GFP in psb1c3. Ratio of ligation 1:1:1 and 2:1:1 Fnr:GFP:psb1c3. <br>
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Result: Transformation in E.coli did not work.<br>
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Nisa<br>
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alsT promoter PCR -->OK! Proceeding to ligation and transformation tomorrow.<br>
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Emeraldo<br>
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Another terminator+biobrick suffix and prefix PCR, cut the purified PCR product and pSac-Cm with EcoRI and HindIII, ligate both cut product and then transform E. coli with the ligated product. <br>
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Latest revision as of 13:31, 26 September 2012







Tom

Ligation of Fnr promotor + GFP in psb1c3. Ratio of ligation 1:1:1 and 2:1:1 Fnr:GFP:psb1c3.

Result: Transformation in E.coli did not work.

Nisa

alsT promoter PCR -->OK! Proceeding to ligation and transformation tomorrow.


Emeraldo

Another terminator+biobrick suffix and prefix PCR, cut the purified PCR product and pSac-Cm with EcoRI and HindIII, ligate both cut product and then transform E. coli with the ligated product.

Back to notebook

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