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| | <html> | | <html> |
| - | <head> | + | <head> |
| - | <style> | + | <style> |
| - | body {
| + | z1 { |
| - | font-family: Helvetica, Arial, sans-serif;
| + | font-size:18pt; |
| - | }
| + | line-height:21pt; |
| - | z1 {
| + | color:white; |
| - | font-size:18pt;
| + | } |
| - | line-height:21pt; | + | z2 { |
| - | color:white; | + | font-size:18pt; |
| - | }
| + | line-height:21pt; |
| - | .ctd {
| + | color:white; |
| - | width: 220px;
| + | } |
| - | text-align: center;
| + | div.ctd { |
| - | background: #000;
| + | width: 90px; |
| - | background-color: transparent;
| + | text-align: center; |
| - | background-color: rgba(0,0,0,0.3);
| + | background: #000; |
| - | padding: 5px ;
| + | background-color: rgba(0,0,0,0.3); |
| - | margin: 30px auto;
| + | padding: 5px ; |
| - | color: #FFF;
| + | margin: 30px auto; |
| - | }
| + | color: #FFF; |
| - | .cte {
| + | } |
| - | width: 280px;
| + | div.cte { |
| - | text-align: center;
| + | width: 120px; |
| - | background: #000;
| + | text-align: center; |
| - | padding-right:10px;
| + | background: #000; |
| - | padding-left:10px;
| + | padding-right:10px; |
| - | background-color: transparent;
| + | padding-left:10px; |
| - | background-color: rgba(0,0,0,0.3);
| + | margin: 0 auto; |
| - | margin:0px auto;
| + | background-color: rgba(0,0,0,0.3); |
| - | color: #FFF;
| + | color: #FFF; |
| - | }
| + | } |
| - | p{ | + | div.ctd2 { |
| - | margin-left:150px; | + | width: 300px; |
| | + | height: 20px; |
| | + | text-align: center; |
| | + | background: #000; |
| | + | background-color: transparent; |
| | + | background-color: rgba(0,0,0,0.3); |
| | + | padding: 10px ; |
| | + | margin: 30px auto; |
| | + | color: #FFF; |
| | + | } |
| | + | div.cte2 { |
| | + | width: 330px; |
| | + | height: 40px; |
| | + | text-align: center; |
| | + | background: #000; |
| | + | padding-right:10px; |
| | + | padding-left:10px; |
| | + | background-color: transparent; |
| | + | background-color: rgba(0,0,0,0.3); |
| | + | margin: -30px auto; |
| | + | color: #FFF; |
| | + | } |
| | + | z7{ |
| | + | font-size:13pt; |
| | + | line-height:14pt; |
| | + | color: rgb(255,103,0); |
| | + | } |
| | + | p.nomargin{ |
| | font-size:12pt; | | font-size:12pt; |
| | line-height:14pt; | | line-height:14pt; |
| | + | margin-right: 0px; |
| | + | margin-left: 0px; |
| | + | color: white; |
| | + | } |
| | + | p.marginleft{ |
| | + | margin-top: 0px; |
| | + | margin-left:150px; |
| | + | font-size:13pt; |
| | + | line-height:15pt; |
| | margin-right:150px; | | margin-right:150px; |
| | color:white; | | color:white; |
| | } | | } |
| - | p.marginleft{
| + | p.orange{ |
| - | font-size:12pt;
| + | |
| - | line-height:14pt;
| + | |
| - | color:white;
| + | |
| | margin-top:0px; | | margin-top:0px; |
| - | margin-bottom:38px;
| |
| - | margin-right:0px;
| |
| | margin-left:150px; | | margin-left:150px; |
| | + | font-size:14pt; |
| | + | line-height:16pt; |
| | margin-right:150px; | | margin-right:150px; |
| | + | color: #FF6700; |
| | + | font-weight: bold; |
| | } | | } |
| - | z2 {
| + | p.marginspace{ |
| - | font-size:18pt; | + | font-size:13pt; |
| - | line-height:18pt; | + | line-height:15pt; |
| - | color:white; | + | color:white; |
| - | background-color: rgba(0,0,0,0.3); | + | |
| | + | margin-top:0px; |
| | + | margin-bottom:200px; |
| | + | margin-right:0px; |
| | + | margin-left:300px; |
| | + | margin-right:300px; |
| | } | | } |
| - | z3 {
| + | p.centered{ |
| - | font-size:18pt; | + | font-size:13pt; |
| - | line-height:18pt; | + | line-height:15pt; |
| | color:white; | | color:white; |
| - | background-color: rgba(0,0,0,0.3);
| |
| | | | |
| | + | margin-top:0px; |
| | + | margin-bottom:0px; |
| | + | margin-right:300px; |
| | + | margin-left:300px; |
| | } | | } |
| - | z4 {
| + | a.inlink{ |
| - | font-size:14pt; | + | font-size: 12pt; |
| - | font-weight: bold; | + | line-height: 12pt; |
| | + | color: rgb(255,103,0); |
| | + | } |
| | + | a.menulink{ |
| | + | font-size:12pt; |
| | + | line-height:12pt; |
| | color:white; | | color:white; |
| | + | font-weight: 500; |
| | } | | } |
| - | ol.ref{
| + | a.menulink:hover{ |
| - | list-style-type: decimal;
| + | |
| - | margin-left:170px;
| + | |
| - | margin-right:150px;
| + | |
| | font-size:12pt; | | font-size:12pt; |
| - | color:white; | + | line-height:12pt; |
| | + | color: rgb(255,103,0); |
| | + | font-weight: 700; |
| | + | } |
| | + | a:link { |
| | + | color: #FFFFFF; |
| | + | } |
| | + | a:visited { |
| | + | color: #FFFFFF; |
| | + | } |
| | + | a:hover { |
| | + | color: #FF6700; |
| | + | } |
| | + | a:active { |
| | + | color: #FF6700; |
| | + | } |
| | + | table.projectmenu{ |
| | + | background-color: transparent; |
| | } | | } |
| - | </style>
| + | td.menucell{ |
| - | </head>
| + | |
| - | <div class="cte">
| + | |
| - | <div class="ctd">
| + | |
| - | <z1>About Us</z1><br>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | </body>
| + | |
| - | | + | |
| - | <p>for enquires please email us at: team(at)igemgroningen.com</p>
| + | |
| - | <p class="margin"></p>
| + | |
| - | </body>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | <html>
| + | |
| - | <div style="width: 100%; top:300px; position:absolute;">
| + | |
| - | <a href="https://2012.igem.org/Team:Groningen/Team/Elbrich" onMouseOver="document.elbrich.src='https://static.igem.org/mediawiki/2012/6/61/AD_20120802_Elbrich2.png';" onMouseOut="document.elbrich.src='https://static.igem.org/mediawiki/2012/4/42/AD_20120802_Elbrich.png';">
| + | |
| - | <img style="margin: auto; border: 0px double black;" name="elbrich" src="https://static.igem.org/mediawiki/2012/4/42/AD_20120802_Elbrich.png" ></a></div>
| + | |
| - | | + | |
| - | </html>
| + | |
| - | | + | |
| - | | + | |
| - | <html>
| + | |
| - | <div style="width: 100%; top:300px;position:absolute;margin-left: 170px;">
| + | |
| - | <a href="https://2012.igem.org/Team:Groningen/Team/Renske" onMouseOver="document.renske.src='https://static.igem.org/mediawiki/2012/6/68/AD_20120819_Renske2.png';" onMouseOut="document.renske.src='https://static.igem.org/mediawiki/2012/4/48/AD_20120819_Renske.png';">
| + | |
| - | <img style=" border: 0px double black;" name="renske" src="https://static.igem.org/mediawiki/2012/4/48/AD_20120819_Renske.png" ></a></div>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | | + | |
| - | <html>
| + | |
| - | <div style="width: 100%; top:300px;position:absolute;margin-left: 340px;">
| + | |
| - | <a href="https://2012.igem.org/Team:Groningen/Team/Wlada"
| + | |
| - | onMouseOver="document.wlada.src='https://static.igem.org/mediawiki/2012/0/06/AD_20120802_Wlada2.png';"
| + | |
| - | onMouseOut="document.wlada.src='https://static.igem.org/mediawiki/2012/b/b3/AD_20120802_Wlada.png';">
| + | |
| - | <img style=" border: 0px double black;" name="wlada" src="https://static.igem.org/mediawiki/2012/b/b3/AD_20120802_Wlada.png" ></a></div>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <div style="width: 100%; top:300px;position:absolute;margin-left: 510px;">
| + | |
| - | <a href="https://2012.igem.org/Team:Groningen/Team/Ismael"
| + | |
| - | onMouseOver="document.ismael.src='https://static.igem.org/mediawiki/2012/8/84/AD_20120802_Ismael2.png';" onMouseOut="document.ismael.src='https://static.igem.org/mediawiki/2012/8/8b/AD_20120802_Ismael.png';">
| + | |
| - | <img style=" border: 0px double black;" name="ismael" src="https://static.igem.org/mediawiki/2012/8/8b/AD_20120802_Ismael.png" ></a></div>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | | + | |
| - | <html>
| + | |
| - | <div style="width: 100%; top:300px;position:absolute;margin-left: 680px;">
| + | |
| - | <a href="https://2012.igem.org/Team:Groningen/Team/Alicja"
| + | |
| - | onMouseOver="document.alicja.src='https://static.igem.org/mediawiki/2012/7/7f/AD_20120802_Alicja2.png';" onMouseOut="document.alicja.src='https://static.igem.org/mediawiki/2012/9/93/AD_20120802_Alicja.png';">
| + | |
| - | <img style=" border: 0px double black;" name="alicja" src="https://static.igem.org/mediawiki/2012/9/93/AD_20120802_Alicja.png" ></a></div>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <div style="width: 100%; top:300px;position:absolute;margin-left: 850px;">
| + | |
| - | <a href="https://2012.igem.org/Team:Groningen/Team/Jon"
| + | |
| - | onMouseOver="document.jon.src='https://static.igem.org/mediawiki/2012/0/01/AD_20120802_Jon2.png';" onMouseOut="document.jon.src='https://static.igem.org/mediawiki/2012/1/12/AD_20120802_Jon.png';">
| + | |
| - | <img style=" border: 0px double black;" name="jon" src="https://static.igem.org/mediawiki/2012/1/12/AD_20120802_Jon.png" ></a></div>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | | + | |
| - | <html>
| + | |
| - | <div style="width: 100%; top:610px;position:absolute;margin-left: 70px;">
| + | |
| - | <a href="https://2012.igem.org/Team:Groningen/Team/Arjan"
| + | |
| - | onMouseOver="document.arjan.src='https://static.igem.org/mediawiki/2012/e/e9/AD_20120802_Arjan2.png';" onMouseOut="document.arjan.src='https://static.igem.org/mediawiki/2012/8/87/AD_20120802_Arjan.png';">
| + | |
| - | <img style=" border: 0px double black;" name="arjan" src="https://static.igem.org/mediawiki/2012/8/87/AD_20120802_Arjan.png" ></a></div>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <div style="width: 100%; top:610px;position:absolute;margin-left: 240px;">
| + | |
| - | <a href="https://2012.igem.org/Team:Groningen/Team/Nisa"
| + | |
| - | onMouseOver="document.nisa.src='https://static.igem.org/mediawiki/2012/7/7a/AD_20120802_Nisa2.png';" onMouseOut="document.nisa.src='https://static.igem.org/mediawiki/2012/e/e4/AD_20120802_Nisa.png';">
| + | |
| - | <img style=" border: 0px double black;" name="nisa" src="https://static.igem.org/mediawiki/2012/e/e4/AD_20120802_Nisa.png" ></a></div>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <div style="width: 100%; top:610px;position:absolute;margin-left: 410px;">
| + | |
| - | <a href="https://2012.igem.org/Team:Groningen/Team/Emeraldo"
| + | |
| - | onMouseOver="document.ema.src='https://static.igem.org/mediawiki/2012/6/6f/AD_20120802_Emeraldo2.png';" onMouseOut="document.ema.src='https://static.igem.org/mediawiki/2012/0/0d/AD_20120802_Emeraldo.png';">
| + | |
| - | <img style=" border: 0px double black;" name="ema" src="https://static.igem.org/mediawiki/2012/0/0d/AD_20120802_Emeraldo.png" ></a></div>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <div style="width: 100%; top:610px;position:absolute;margin-left: 580px;">
| + | |
| - | <a href="https://2012.igem.org/Team:Groningen/Team/Tom"
| + | |
| - | onMouseOver="document.tom.src='https://static.igem.org/mediawiki/2012/3/30/AD_20120802_Tom2.png';" onMouseOut="document.tom.src='https://static.igem.org/mediawiki/2012/3/39/AD_20120802_Tom.png';">
| + | |
| - | <img style=" border: 0px double black;" name="tom" src="https://static.igem.org/mediawiki/2012/3/39/AD_20120802_Tom.png" ></a></div>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <div style="width: 100%; top:610px;position:absolute;margin-left: 750px;">
| + | |
| - | <a href="https://2012.igem.org/Team:Groningen/Team/Yonathan"
| + | |
| - | onMouseOver="document.yon.src='https://static.igem.org/mediawiki/2012/c/cc/AD_20120802_Yonathan2.png';" onMouseOut="document.yon.src='https://static.igem.org/mediawiki/2012/b/bf/AD_20120802_Yonathan.png';">
| + | |
| - | <img style=" border: 0px double black;" name="yon" src="https://static.igem.org/mediawiki/2012/b/bf/AD_20120802_Yonathan.png" ></a></div>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | <html>
| + | |
| - | <head>
| + | |
| - | <style>
| + | |
| - | | + | |
| - | body {
| + | |
| - | font-family: Helvetica, Arial, sans-serif;
| + | |
| - | | + | |
| - | }
| + | |
| - | elbrichP {
| + | |
| - | font-size:12pt;
| + | |
| - | line-height:12pt;
| + | |
| - | color:white;
| + | |
| - | }
| + | |
| - | .elbrich1 {
| + | |
| - | width: 110px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | padding: 10px ;
| + | |
| - | margin: 0px auto;
| + | |
| - | color: #FFF;
| + | |
| - | }
| + | |
| - | .elbrich2 {
| + | |
| - | left: 0px;
| + | |
| - | top:550px;
| + | |
| - | position:absolute;
| + | |
| - | width: 130px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | padding-right:10px;
| + | |
| - | padding-left:10px;
| + | |
| - | margin: 0 auto;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | color: #FFF;
| + | |
| - | | + | |
| - | }
| + | |
| - | </style>
| + | |
| - | | + | |
| - | <div class="elbrich2">
| + | |
| - | <div class="elbrich1">
| + | |
| - | <elbrichP ><A HREF="https://2012.igem.org/Team:Groningen/Team/Elbrich" TARGET="_blank"><FONT COLOR=#ffffff>Elbrich<br>team leader</FONT></A></elbrichP><br>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | </body>
| + | |
| - | </head>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | <html>
| + | |
| - | <head>
| + | |
| - | <style>
| + | |
| - | | + | |
| - | body {
| + | |
| - | font-family: Helvetica, Arial, sans-serif;
| + | |
| - | | + | |
| - | }
| + | |
| - | renskeP {
| + | |
| - | font-size:12pt;
| + | |
| - | line-height:12pt;
| + | |
| - | color:white;
| + | |
| - | }
| + | |
| - | .renske1 {
| + | |
| - | width: 110px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | padding: 10px ;
| + | |
| - | margin: 0px auto;
| + | |
| - | color: #FFF;
| + | |
| - | }
| + | |
| - | .renske2 {
| + | |
| - | left: 170px;
| + | |
| - | top:550px;
| + | |
| - | position:absolute;
| + | |
| - | width: 130px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | padding-right:10px;
| + | |
| - | padding-left:10px;
| + | |
| - | margin: 0 auto;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | color: #FFF;
| + | |
| - | | + | |
| - | }
| + | |
| - | </style>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="renske2">
| + | |
| - | <div class="renske1">
| + | |
| - | <renskeP ><A HREF="https://2012.igem.org/Team:Groningen/Team/Renske" TARGET="_blank"><FONT COLOR=#ffffff>Renske<br>secretary / PR </FONT></A></renskeP>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | </body>
| + | |
| - | </head>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <head>
| + | |
| - | <style>
| + | |
| - | | + | |
| - | body {
| + | |
| - | font-family: Helvetica, Arial, sans-serif;
| + | |
| - | | + | |
| - | }
| + | |
| - | WladaP {
| + | |
| - | font-size:12pt;
| + | |
| - | line-height:12pt;
| + | |
| - | color:white;
| + | |
| - | }
| + | |
| - | .Wlada1 {
| + | |
| - | width: 110px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | padding: 10px ;
| + | |
| - | margin: 0px auto;
| + | |
| - | color: #FFF;
| + | |
| - | }
| + | |
| - | .Wlada2 {
| + | |
| - | left: 340px;
| + | |
| - | top:550px;
| + | |
| - | position:absolute;
| + | |
| - | width: 130px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | padding-right:10px;
| + | |
| - | padding-left:10px;
| + | |
| - | margin: 0 auto;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | color: #FFF;
| + | |
| - | | + | |
| - | }
| + | |
| - | </style>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="Wlada2">
| + | |
| - | <div class="Wlada1">
| + | |
| - | <WladaP ><A HREF="https://2012.igem.org/Team:Groningen/Team/Wlada" TARGET="_blank"><FONT COLOR=#ffffff>Wlada<br>treasurer</FONT></A></WladaP><br>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | </body>
| + | |
| - | </head>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <head>
| + | |
| - | <style>
| + | |
| - | | + | |
| - | body {
| + | |
| - | font-family: Helvetica, Arial, sans-serif;
| + | |
| - | | + | |
| - | }
| + | |
| - | IsmaelP {
| + | |
| - | font-size:12pt;
| + | |
| - | line-height:12pt;
| + | |
| - | color:white;
| + | |
| - | }
| + | |
| - | .Ismael1 {
| + | |
| - | width: 110px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | padding: 10px ;
| + | |
| - | margin: 0px auto;
| + | |
| - | color: #FFF;
| + | |
| - | }
| + | |
| - | .Ismael2 {
| + | |
| - | left: 510px;
| + | |
| - | top:550px;
| + | |
| - | position:absolute;
| + | |
| - | width: 130px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | padding-right:10px;
| + | |
| - | padding-left:10px;
| + | |
| - | margin: 0 auto;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | color: #FFF;
| + | |
| - | | + | |
| - | }
| + | |
| - | </style>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="Ismael2">
| + | |
| - | <div class="Ismael1">
| + | |
| - | <IsmaelP ><A HREF="https://2012.igem.org/Team:Groningen/Team/Ismael" TARGET="_blank"><FONT COLOR=#ffffff>Ismaêl<br>web developer</FONT></A></IsmaelP>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | </body>
| + | |
| - | </head>
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| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <head>
| + | |
| - | <style>
| + | |
| - | | + | |
| - | body {
| + | |
| - | font-family: Helvetica, Arial, sans-serif;
| + | |
| - | | + | |
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| + | |
| - | AlicjaP {
| + | |
| - | font-size:12pt;
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| - | line-height:12pt;
| + | |
| - | color:white;
| + | |
| - | }
| + | |
| - | .Alicja1 {
| + | |
| - | width: 110px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | padding: 10px ;
| + | |
| - | margin: 0px auto;
| + | |
| - | color: #FFF;
| + | |
| - | }
| + | |
| - | .Alicja2 {
| + | |
| - | left: 680px;
| + | |
| - | top:550px;
| + | |
| - | position:absolute;
| + | |
| - | width: 130px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | padding-right:10px;
| + | |
| - | padding-left:10px;
| + | |
| - | margin: 0 auto;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | color: #FFF;
| + | |
| - | | + | |
| - | }
| + | |
| - | </style>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="Alicja2">
| + | |
| - | <div class="Alicja1">
| + | |
| - | <AlicjaP ><A HREF="https://2012.igem.org/Team:Groningen/Team/Alicja" TARGET="_blank"><FONT COLOR=#ffffff>Alicja<br>web designer</FONT></A></AlicjaP>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | </body>
| + | |
| - | </head>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <head>
| + | |
| - | <style>
| + | |
| - | | + | |
| - | body {
| + | |
| - | font-family: Helvetica, Arial, sans-serif;
| + | |
| - | | + | |
| - | }
| + | |
| - | JonP {
| + | |
| - | font-size:12pt;
| + | |
| - | line-height:12pt;
| + | |
| - | color:white;
| + | |
| - | }
| + | |
| - | .Jon1 {
| + | |
| - | width: 110px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | padding: 10px ;
| + | |
| - | margin: 0px auto;
| + | |
| - | color: #FFF;
| + | |
| - | }
| + | |
| - | .Jon2 {
| + | |
| - | left: 850px;
| + | |
| - | top:550px;
| + | |
| - | position:absolute;
| + | |
| - | width: 130px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | padding-right:10px;
| + | |
| - | padding-left:10px;
| + | |
| - | margin: 0 auto;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | color: #FFF;
| + | |
| - | | + | |
| - | }
| + | |
| - | </style>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="Jon2">
| + | |
| - | <div class="Jon1">
| + | |
| - | <JonP ><A HREF="https://2012.igem.org/Team:Groningen/Team/Jon" TARGET="_blank"><FONT COLOR=#ffffff>Jonathan<br>modeler</FONT></A></JonP><br>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | </body>
| + | |
| - | </head>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <head>
| + | |
| - | <style>
| + | |
| - | | + | |
| - | body {
| + | |
| - | font-family: Helvetica, Arial, sans-serif;
| + | |
| - | | + | |
| - | }
| + | |
| - | ArjanP {
| + | |
| - | font-size:12pt;
| + | |
| - | line-height:12pt;
| + | |
| - | color:white;
| + | |
| - | }
| + | |
| - | .Arjan1 {
| + | |
| - | width: 110px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | padding: 10px ;
| + | |
| - | margin: 0px auto;
| + | |
| - | color: #FFF;
| + | |
| - | }
| + | |
| - | .Arjan2 {
| + | |
| - | left: 70px;
| + | |
| - | top:860px;
| + | |
| - | position:absolute;
| + | |
| - | width: 130px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | padding-right:10px;
| + | |
| - | padding-left:10px;
| + | |
| - | margin: 0 auto;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | color: #FFF;
| + | |
| - | | + | |
| - | }
| + | |
| - | </style>
| + | |
| - | | + | |
| - | <div class="Arjan2">
| + | |
| - | <div class="Arjan1">
| + | |
| - | <ArjanP ><A HREF="https://2012.igem.org/Team:Groningen/Team/Arjan" TARGET="_blank"><FONT COLOR=#ffffff>Arjan<br>lab worker</FONT></A></ArjanP>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | </body>
| + | |
| - | </head>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <head>
| + | |
| - | <style>
| + | |
| - | | + | |
| - | body {
| + | |
| - | font-family: Helvetica, Arial, sans-serif;
| + | |
| - | | + | |
| - | }
| + | |
| - | NisaP {
| + | |
| - | font-size:12pt;
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| - | line-height:12pt;
| + | |
| - | color:white;
| + | |
| - | }
| + | |
| - | .Nisa1 {
| + | |
| - | width: 110px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | padding: 10px ;
| + | |
| - | margin: 0px auto;
| + | |
| - | color: #FFF;
| + | |
| - | }
| + | |
| - | .Nisa2 {
| + | |
| - | left: 240px;
| + | |
| - | top:860px;
| + | |
| - | position:absolute;
| + | |
| - | width: 130px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | padding-right:10px;
| + | |
| - | padding-left:10px;
| + | |
| - | margin: 0 auto;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | color: #FFF;
| + | |
| - | | + | |
| - | }
| + | |
| - | </style>
| + | |
| - | | + | |
| - | <div class="Nisa2">
| + | |
| - | <div class="Nisa1">
| + | |
| - | <NisaP ><A HREF="https://2012.igem.org/Team:Groningen/Team/Nisa" TARGET="_blank"><FONT COLOR=#ffffff>Nisa<br>lab worker</FONT></A></NisaP><br>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | </body>
| + | |
| - | </head>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <head>
| + | |
| - | <style>
| + | |
| - | | + | |
| - | body {
| + | |
| - | font-family: Helvetica, Arial, sans-serif;
| + | |
| - | | + | |
| - | }
| + | |
| - | EmaP {
| + | |
| - | font-size:12pt;
| + | |
| - | line-height:12pt;
| + | |
| - | color:white;
| + | |
| - | }
| + | |
| - | .Ema1 {
| + | |
| - | width: 110px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | padding: 10px ;
| + | |
| - | margin: 0px auto;
| + | |
| - | color: #FFF;
| + | |
| - | }
| + | |
| - | .Ema2 {
| + | |
| - | left: 410px;
| + | |
| - | top:860px;
| + | |
| - | position:absolute;
| + | |
| - | width: 130px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | padding-right:10px;
| + | |
| - | padding-left:10px;
| + | |
| - | margin: 0 auto;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | color: #FFF;
| + | |
| - | | + | |
| - | }
| + | |
| - | </style>
| + | |
| - | | + | |
| - | <div class="Ema2">
| + | |
| - | <div class="Ema1">
| + | |
| - | <EmaP ><A HREF="https://2012.igem.org/Team:Groningen/Team/Emeraldo" TARGET="_blank"><FONT COLOR=#ffffff>Emeraldo<br>lab worker</FONT></A></EmaP>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | </body>
| + | |
| - | </head>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <head>
| + | |
| - | <style>
| + | |
| - | | + | |
| - | body {
| + | |
| - | font-family: Helvetica, Arial, sans-serif;
| + | |
| - | | + | |
| - | }
| + | |
| - | TomP {
| + | |
| - | font-size:12pt;
| + | |
| - | line-height:12pt;
| + | |
| - | color:white;
| + | |
| - | }
| + | |
| - | .Tom1 {
| + | |
| - | width: 110px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | padding: 10px ;
| + | |
| - | margin: 0px auto;
| + | |
| - | color: #FFF;
| + | |
| - | }
| + | |
| - | .Tom2 {
| + | |
| - | left: 580px;
| + | |
| - | top:860px;
| + | |
| - | position:absolute;
| + | |
| - | width: 130px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | padding-right:10px;
| + | |
| - | padding-left:10px;
| + | |
| - | margin: 0 auto;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | color: #FFF;
| + | |
| - | | + | |
| - | }
| + | |
| - | </style>
| + | |
| - | | + | |
| - | <div class="Tom2">
| + | |
| - | <div class="Tom1">
| + | |
| - | <TomP ><A HREF="https://2012.igem.org/Team:Groningen/Team/Tom" TARGET="_blank"><FONT COLOR=#ffffff>Tom<br>lab worker</FONT></A></TomP>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | </body>
| + | |
| - | </head>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | <html>
| + | |
| - | <head>
| + | |
| - | <style>
| + | |
| - | | + | |
| - | body {
| + | |
| - | font-family: Helvetica, Arial, sans-serif;
| + | |
| - | | + | |
| - | }
| + | |
| - | YonP {
| + | |
| - | font-size:12pt;
| + | |
| - | line-height:12pt;
| + | |
| - | color:white;
| + | |
| - | }
| + | |
| - | .Yon1 {
| + | |
| - | width: 110px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | padding: 10px ;
| + | |
| - | margin: 0px auto;
| + | |
| - | color: #FFF;
| + | |
| - | }
| + | |
| - | .Yon2 {
| + | |
| - | left: 750px;
| + | |
| - | top:860px;
| + | |
| - | position:absolute;
| + | |
| - | width: 130px;
| + | |
| - | text-align: center;
| + | |
| - | background: #000;
| + | |
| - | padding-right:10px;
| + | |
| - | padding-left:10px;
| + | |
| - | margin: 0 auto;
| + | |
| - | background-color: transparent;
| + | |
| - | background-color: rgba(0,0,0,0.3);
| + | |
| - | color: #FFF;
| + | |
| - | | + | |
| - | }
| + | |
| - | </style>
| + | |
| - | | + | |
| - | <div class="Yon2">
| + | |
| - | <div class="Yon1">
| + | |
| - | <YonP ><A HREF="https://2012.igem.org/Team:Groningen/Team/Yonathan" TARGET="_blank"><FONT COLOR=#ffffff>Yonathan<br>lab worker</FONT></A></YonP>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | </body>
| + | |
| - | </head>
| + | |
| - | </html>
| + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | <html>
| + | |
| - | <head>
| + | |
| - | <style>
| + | |
| - | | + | |
| - | body {
| + | |
| - | font-family: Helvetica, Arial, sans-serif;
| + | |
| - | | + | |
| - | }
| + | |
| - | z2 {
| + | |
| - | font-size:21pt; | + | |
| - | line-height:21pt;
| + | |
| - | color:white;
| + | |
| - | }
| + | |
| - | .ctf {
| + | |
| | | | |
| - | | + | } |
| - | width: 440px;
| + | table.centertable{ |
| - | text-align: center;
| + | background-color: transparent; |
| - | background: #000;
| + | width: 100%; |
| - | background-color: transparent;
| + | margin-top: 0; |
| - | background-color: rgba(0,0,0,0.3);
| + | margin-bottom: 0; |
| - | padding: 10px ;
| + | } |
| - | margin: 0px auto;
| + | td.margincell{ |
| - |
| + | min-width: 150px; |
| - | color: #FFF;
| + | max-width: 150px; |
| | + | vertical-align: bottom; |
| | + | } |
| | + | div.bigcog{ |
| | + | opacity:0.4; |
| | + | filter:alpha(opacity=40); |
| | + | z-index: -1; |
| | + | } |
| | + | #bigcogtopleft{ |
| | + | position: relative; |
| | + | left: -10px; |
| | + | } |
| | + | #bigcogtopright{ |
| | + | position: relative; |
| | + | left: 10px; |
| | + | } |
| | + | div.cogoverlay{ |
| | + | z-index: 1; |
| | + | } |
| | + | #cogoverlaytopleft{ |
| | + | position: relative; |
| | + | left: -350px; |
| | + | top: -150px; |
| | + | } |
| | + | #cogoverlaytopright{ |
| | + | position: relative; |
| | + | left: 355px; |
| | + | top: -150px; |
| | + | } |
| | + | #cogoverlaybottomleft{ |
| | + | position: relative; |
| | + | top: -25px; |
| | + | left: 185px; |
| | + | } |
| | + | #cogoverlaybottomright{ |
| | + | position: relative; |
| | + | top: -25px; |
| | + | left: -135px; |
| | + | } |
| | + | </style> |
| | + | </head> |
| | | | |
| - | }
| + | <body> |
| - | .ctg {
| + | <div class="cte"> |
| - | left: 250px; | + | <div class="ctd"> |
| - | top:950px; | + | <z1>Abstract</z1> |
| - | position:absolute; | + | </div> |
| - | | + | </div> |
| - | width: 500px;
| + | <table class="centertable"> |
| - | text-align: center; | + | <tr> |
| - | background: #000;
| + | <td class="margincell" align="right"> |
| - | padding-right:10px; | + | <div class="bigcog" id="bigcogtopleft"> |
| - | padding-left:10px; | + | <img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150px" height="150px"> |
| - | margin: 0 auto;
| + | </div> |
| - | background-color: transparent; | + | </td> |
| - | background-color: rgba(0,0,0,0.3); | + | <td colspan="4"> |
| - | color: #FFF;
| + | <p class="nomargin"> |
| | + | Every year, one third of global food production -1.3 billion tons of food- is thrown away, partially due to the “best before” dating system. |
| | + | <z7>iGEM Groningen 2012</z7> seeks to provide an alternative method of assessing edibility: the <z7>Food Warden</z7>. It uses an <z7>engineered strain</z7> |
| | + | of <i>Bacillus subtilis</i> to detect and report volatiles in spoiling meat. The introduced <z7>genetic construct</z7> uses a promoter to trigger |
| | + | a pigment coding gene. This promoter, <z7>identified by microarray analysis</z7>, is significantly upregulated in the presence of |
| | + | <z7>volatiles from spoiling meat</z7>. The activity of the <z7>promoter</z7> regulates the expression of the <z7>pigment reporter</z7> and will |
| | + | be visible to the naked eye. For safe usage of the system, spores of our engineered strain are placed into one half of a semi-permeable |
| | + | <z7>capsule</z7>, the second containing a calibrated amount of nutrients. Breaking the barrier between the two compartments allows |
| | + | <z7>germination and growth</z7>, thereby activating the <z7>spoiling-meat sensor</z7>. |
| | | | |
| - | } | + | </p> |
| - | </style> | + | </td> |
| - | | + | <td class="margincell" align="left"> |
| - | | + | <div class="bigcog" id="bigcogtopright"> |
| - | <div class="ctg"> | + | <img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150px" height="150px"> |
| - | <div class="ctf"> | + | </div> |
| - | <z2 >ADVISORS & SUPERVISORS</z2><br> | + | </td> |
| - | </div> | + | </tr> |
| - | </div> | + | <tr> |
| - | </body> | + | <td class="margincell"> |
| - | </head> | + | <div class=" cogoverlay" id="cogoverlaybottomleft"> |
| | + | <img src="https://static.igem.org/mediawiki/2012/c/c2/Groningen2012_RR_20120909_rotting.png" width="100" height="100"> |
| | + | </div> |
| | + | </td> |
| | + | <td align="left" > |
| | + | <div class="bigcog" id="bigcogbottomleft"> |
| | + | <img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150px" height="150px"> |
| | + | </div> |
| | + | </td> |
| | + | <td align="left" > |
| | + | <div class="cogoverlay" id="cogoverlaytopleft"> |
| | + | <img src="https://static.igem.org/mediawiki/2012/1/15/Groningen2012_RR20120909_construct.png" width="100px" height="100px"> |
| | + | </div> |
| | + | </td> |
| | + | <td align="right" > |
| | + | <div class="cogoverlay" id="cogoverlaytopright"> |
| | + | <img src="https://static.igem.org/mediawiki/2012/2/29/Groningen2012_RR20120909_badmeat.png" width="100px" height="100px"> |
| | + | </div> |
| | + | </td> |
| | + | <td align="right"> |
| | + | <div class="bigcog" id="bigcogbottomright"> |
| | + | <img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150px" height="150px"> |
| | + | </div> |
| | + | </td> |
| | + | <td class="margincell"> |
| | + | <div class="cogoverlay" id="cogoverlaybottomright"> |
| | + | <img src="https://static.igem.org/mediawiki/2012/e/e7/Groningen2012_RR20120909_capsule.png" width="100px" height="100px"> |
| | + | </div> |
| | + | </td> |
| | + | </tr> |
| | + | </table> |
| | + | <br> |
| | + | <br> |
| | + | <div class="cte2"> |
| | + | <div class="ctd2"> |
| | + | <a name="MainAcc"></a><z2 >Our main accomplishments</z2><br> |
| | + | </div> |
| | + | </div> |
| | + | <br><br> |
| | + | <p class=orange>In the lab</p> |
| | + | <p class="marginleft"> |
| | + | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Most importantly: we developed a construct which makes <i>Bacillus subtilis</i> sense spoiled meat and produce an output in the form of a yellow pigment visible by naked eye.<br><br> |
| | + | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Design of the “<a href="https://2012.igem.org/Team:Groningen/Sticker">Sticker</a>”: semi-permeable capsule: bacteria are kept inside, volatiles can go through. Proof that <i>Bacillus subtilis</i> grows inside the sticker.<br><br> |
| | + | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Development of BioBrick psac-cm for <i>Bacillus subtilis</i>. <br><a href="http://partsregistry.org/Part:BBa_K818000" target=_blank><font size=4 color=#FF6700><b>Easy cloning in <i>Bacillus subtilis</i> in Biobrick fashion for the first time!</b></font></a> Advantages: easy to check, BioBrick compatible, <i>E. coli</i> compatible, stable insertion into <i>Bacillus subtilis</i> chromosome.<br><br> |
| | + | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Identification of spoiled meat sensors by transcriptome analysis. Usage of an innovative experimental setup which can be used to test more sensors in the future.<br><br> |
| | + | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We made AmilCP and AmilGFP be expressed in <i>Bacillus subtilis</i>. These chromoproteins can be of significant value to the other <i>Bacillus subtilis</i> users in the BioBrick community.<br><br><br> |
| | + | </p> |
| | + | <p class=orange>Other</p> |
| | + | <p class="marginleft"> |
| | + | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Smart spores activation system: inspired by "glow in the dark stick", we designed a sticker with two compartments. One compartment holds the spores, the other the nutrients; when the nutrients compartment is braked, the spores start to germinate, activating the sticker.<br><br> |
| | + | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We explored the definition of spoiled meat and different ways to test this.<br><br> |
| | + | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We identified various compounds present in spoiled meat by Gas Chromatography - Mass Spectrometry.<br><br> |
| | + | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We made a model which gives insight on how to tweak the growth of <i>Bacillus subtilis</i> based upon literature, experimental data and flux balance analysis.<br> |
| | + | </p> |
| | + | </body> |
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Most importantly: we developed a construct which makes Bacillus subtilis sense spoiled meat and produce an output in the form of a yellow pigment visible by naked eye.
