Team:OUC-China/HumanPractice/Overview

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<h1>Promoter Engineering</h1>
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<h1 style="border:none;">Overview</h1>
<h2>Abstract</h2>
<h2>Abstract</h2>
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<p>Overloading of phosphorus and nitrogen are the most important causes of red tide. So it is necessary to construct
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<p>Is genetically modified food safe or not? Is genetic engineering or synthetic biology a horrible weapon? What would the world be if we insist on modifying the genetic material of our fellow organisms? Being the species that has made more changes to earth than any other form of life means a lot of duty. Greater ability brings forth the need for further responsibility. We, human beings, must think over what we are doing. Here comes the necessity for human practice.Our work on human practice can be concluded into 3 parts. First, leaving more people with the impression that synthetic biology is not equal to Resident Evil. It’s not only a question of making synthetic biology known to the world; it’s a human right for every person to know what is really going on. As iGEMers, we have the duty to make iGEM known and eliminate misunderstandings about the subject. We have held a number of activities, lectures and camps aimed at children and young adults during the last summer vacation about iGEM and synthetic biology.Second, learning more about lab safety when coming to genetic engineering and paying close attention to safety when do experiments. For this reason, we studied safety on iGEM safety page, searched from the internet and consulted professors. This summer, we contacted QIBEBT and learned how to regulate a molecular lab. Third, reflecting on our humanistic sensibilities. Our final goal is to protect the environment and make a better future through our own hands. We must do experiments environmentally friendly. Synthetic biology is a subject that can modify genes and change the world. We must value all the living things on the earth and treat them with respect.</p>
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<br/>
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<h2>Timeline</h2>
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<p><strong>Nov.2011</strong>: <span></span>Meeting with Peking iGEM Team<br/><br/>
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corresponding sensors able to respond effectively and accurately. Once our design is finished, it could be integrated into
 
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our information-processing devices, a comparator or ratio sensor. We have investigated a lot about the endogenous phosphate
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<p><strong>Nov.2011</strong> : <span></span>A Visit to Institute of Microbiology Chinese Academy of Science<br/><br/>
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sensor and nitrate sensor in prokaryotes. Even though we successfully screened some candidates for further modification, it
 
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is still frustrating that few mechanisms match perfectly with the corresponding phosphate and nitrate concentration when
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<p><strong>Dec.2011</strong> : <span></span>Meeting with Researcher Xu Jian in Ouc iGEM Lab<br/><br/>
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red tide probably occurs. So fine-tuning of nitrate and phosphate sensor is somewhat difficult but necessary. Given the
 
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limited time, we have focused more on the promoter testing platform construction and characterization. Since a variety of
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<p><strong>Feb.2012</strong>:<span></span>Winter camp for brain storming<br/><br/>
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sensors rely on the two-component system(TCS) to function, we also conceive a whole set of design ideas for TCS
 
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modifications, thereby enable other teams to fine-tune their sensors to respond desirably.</p>
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<p><strong>April.2012</strong>:<span></span>Introduce our project to professors and students in our university.<br/>
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<br/>
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<h2>Background</h2>
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  <sub></sub><span></span>Lecture of iGEM in OUC<br/><br/>
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<h3>Overview of two-component system</h3>
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<p>To adapt and survive in different microenvironments, bacteria must sense and respond to extracellular signals. The
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adaptive response to environmental stimuli can be transduced by two-component regulatory systems (Gao and Stock, 2009),
 
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which are involved in the regulation of chemotaxis, osmoregulation, metabolism and transport(Mizuno, 1997). A typical TCS
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<p><strong>June.2012</strong>:<span></span>Popularization of bioscience with young kids in class<br/><br/>
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is comprised of a membrane-bound histidine kinase (HK) and a partner response regulator (RR). Specific environmental
 
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signals lead to activation (autophosphorylation) of the HK, which in turn acts as a phosphoryl donor for activation
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<p><strong>July.2012</strong>:<span></span>Hands-on experience summer camp with Qingdao No.2 High School.<br/>
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            <sub></sub><span></span>Do experiments and make iGEM known in QIBEBT.<br/><br/>
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(autophosphorylation) of the partner RR. Phosphate and nitrate sensors.</p>
 
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<h3>Phosphate sensor----phoBR system</h3>
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<p><strong>Aug.2012</strong>:<span></span>2th summer camp of life science and technology</p>
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<p>the extracellular concentration of phosphate is sensed by the two-component regulatory system PhoBR, in which PhoR
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encodes the HK and PhoB the RR. PhoB, when phosphorylated by PhoR, would form homodimers and bind to the consensus pho-box
 
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sequence involved in the corresponding promoter and activate downstream genes expression. Generally speaking, phoBR system
 
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responds to phosphate limitation, when the extracellular phosphate concentration falls below 4 uM. Though iGEM-2008 team of
 
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NYMU-Taipei has submitted the information of that part, we couldn’t get it from MIT registry. So we decide to acquire and
 
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characterize this part by ourselves.</p>
 
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Latest revision as of 02:23, 25 October 2012

Overview

Abstract

Is genetically modified food safe or not? Is genetic engineering or synthetic biology a horrible weapon? What would the world be if we insist on modifying the genetic material of our fellow organisms? Being the species that has made more changes to earth than any other form of life means a lot of duty. Greater ability brings forth the need for further responsibility. We, human beings, must think over what we are doing. Here comes the necessity for human practice.Our work on human practice can be concluded into 3 parts. First, leaving more people with the impression that synthetic biology is not equal to Resident Evil. It’s not only a question of making synthetic biology known to the world; it’s a human right for every person to know what is really going on. As iGEMers, we have the duty to make iGEM known and eliminate misunderstandings about the subject. We have held a number of activities, lectures and camps aimed at children and young adults during the last summer vacation about iGEM and synthetic biology.Second, learning more about lab safety when coming to genetic engineering and paying close attention to safety when do experiments. For this reason, we studied safety on iGEM safety page, searched from the internet and consulted professors. This summer, we contacted QIBEBT and learned how to regulate a molecular lab. Third, reflecting on our humanistic sensibilities. Our final goal is to protect the environment and make a better future through our own hands. We must do experiments environmentally friendly. Synthetic biology is a subject that can modify genes and change the world. We must value all the living things on the earth and treat them with respect.


Timeline

Nov.2011: Meeting with Peking iGEM Team

Nov.2011 : A Visit to Institute of Microbiology Chinese Academy of Science

Dec.2011 : Meeting with Researcher Xu Jian in Ouc iGEM Lab

Feb.2012:Winter camp for brain storming

April.2012:Introduce our project to professors and students in our university.
Lecture of iGEM in OUC

June.2012:Popularization of bioscience with young kids in class

July.2012:Hands-on experience summer camp with Qingdao No.2 High School.
Do experiments and make iGEM known in QIBEBT.

Aug.2012:2th summer camp of life science and technology

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