Team:Groningen/DesignTest2

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<z1>Festivals</z1><br>
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<z1>Abstract</z1>
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<z5>Night of Arts and Science</z5>  
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2-3 June 2012, Groningen - <a href="http://www.denachtvankunstenwetenschap.nl/en/" target=_blank"><font color=#ff6700>link</font></a>
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<img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150px" height="150px">
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Designer <a class="inlink" href="http://www.leenvanwijngaarden.nl/Leenvanwijngaarden/home.html">Leen van Wijngaarden</a>
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Every year, one third of global food production -1.3 billion tons of food- is thrown away, partially due to the “best before” dating system.
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was fascinated by the colors and shapes of bacterial colonies and decided to make art with them. Not just a pretty picture on a small regular agar plate,
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<z7>iGEM Groningen 2012</z7> seeks to provide an alternative method of assessing edibility: the <z7>Food Warden</z7>. It uses an <z7>engineered strain</z7>  
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like <a class="inlink" href="http://1.bp.blogspot.com/_HijhRK2BkjA/S2ggPYgVgYI/AAAAAAAABhg/fxaE4Lxt93w/s400/onlinepaint.jpg">this</a>
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of <i>Bacillus subtilis</i> to detect and report volatiles in spoiling meat. The introduced <z7>genetic construct</z7> uses a promoter to trigger
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well-known example, but on a large scale. This should produce nice paintings, which keep evolving into "Bacterial Beauties".
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a pigment coding gene. This promoter, <z7>identified by microarray analysis</z7>, is significantly upregulated in the presence of
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iGEM Groningen 2012 assisted with preparing the XL agar plates and painting them with bacteria.
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<z7>volatiles from spoiling meat</z7>. The activity of the <z7>promoter</z7> regulates the expression of the <z7>pigment reporter</z7> and will
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Resulting in this beautiful picture on the Night of Arts and Science (2 June 2012):
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be visible to the naked eye. For safe usage of the system, spores of our engineered strain are placed into one half of a semi-permeable
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</p>
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<z7>capsule</z7>, the second containing a calibrated amount of nutrients. Breaking the barrier between the two compartments allows
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<z7>germination and growth</z7>, thereby activating the <z7>spoiling-meat sensor</z7>.
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<img src="https://static.igem.org/mediawiki/2012/9/99/Groningen2012_RR_20120520_smalldrawingEmNiLeen.jpg" width="150">
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<img src="https://static.igem.org/mediawiki/2012/1/17/Groningen2012_EH_during_night_of_arts_and_science.jpg" width="150">
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Because of the microbiological background of many team members, not only we could help Leen during the making of the pictures,
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but we could also take the opportunity to teach the visitors of the Night about the beauty of bacteria, their use,
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synthetic biology, and our project.
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<img src="https://static.igem.org/mediawiki/2012/c/c2/Groningen2012_RR_20120909_rotting.png" width="100" height="100">
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In addition to the bio-paintings and the huge "bio-billboard", time lapse movies of bacteria growing on a normal
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plate, and microscopic images of bacteria were displayed. Furthermore, to aid in explaining synthetic biology,
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our team showed some paintings with an overview of our project.
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<img src="https://static.igem.org/mediawiki/2012/1/15/Groningen2012_RR20120909_construct.png" width="100px" height="100px">
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<img src="https://static.igem.org/mediawiki/2012/e/e7/Groningen2012_RR20120909_capsule.png" width="100px" height="100px">
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As an extra feature, a "bacterial wall of fame" was built using handprints of "famous people from Groningen".
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People, including some iGEM members, had pressed their hands on agar plates, which were then incubated overnight.
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During the night the diverse skin inhabitants flourished...
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<div class="cte2">
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<a name="MainAcc"></a><z2 >Our main accomplishments</z2><br>
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<z5>Noordezon Festival</z5>
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<br><br>
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16-26 August 2012, Groningen - <a href="http://www.noorderzon.nl"><font color=#ff6700>link</font></a>
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<p class=orange>In the lab</p>
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We assisted Leen again at the preparations of the performing arts festival Noorderzon.
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This annual festival in the city of Groningen draws around 110.000 visitors.
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Every year, the education institutes in Groningen build up a science centre during the festival called “Qu3” (link).
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In front of the Qu3 our bio-billboard was evolving rapidly in the hot, hot weather!
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<img src="https://static.igem.org/mediawiki/2012/f/f8/Groningen2012_RR20120923_Noorderzonsmall.jpg" width="290" >
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<z5>Transnatural</z5>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Most importantly: we developed a construct which makes <i>Bacillus subtilis</i> sense spoiled meat and produce an output in the form of a yellow pigment visible by naked eye.<br><br>
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<br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Design of the “<a href="https://2012.igem.org/Team:Groningen/Sticker">Sticker</a>”: semi-permeable capsule: bacteria are kept inside, volatiles can go through. Proof that <i>Bacillus subtilis</i> grows inside the sticker.<br><br>
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<br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Development of BioBrick psac-cm for <i>Bacillus subtilis</i>. <br><a href="http://partsregistry.org/Part:BBa_K818000" target=_blank><font size=4 color=#FF6700><b>Easy cloning in <i>Bacillus subtilis</i> in Biobrick fashion for the first time!</b></font></a> Advantages: easy to check, BioBrick compatible, <i>E. coli</i> compatible, stable insertion into <i>Bacillus subtilis</i> chromosome.<br><br>
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17-18 September 2012, Amsterdam - <a href="http://www.transnatural.nl/226-Food-Spoilage#A228"><font color=#ff6700>link</font></a>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Identification of spoiled meat sensors by transcriptome analysis. Usage of an innovative experimental setup which can be used to test more sensors in the future.<br><br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We made AmilCP and AmilGFP be expressed in <i>Bacillus subtilis</i>. These chromoproteins can be of significant value to the other <i>Bacillus subtilis</i> users in the BioBrick community.<br><br><br>
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Of the European iGEM teams who applied, we were selected to speak at the conference of the  Transnatural Festival in Nemo, Amsterdam!
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This festival is held every two years and aims to highlight art, science and technology in which state-of-the-art technology goes together with nature.
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This year’s theme was “smart as matter”. You can find some pictures on the presentation page!
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<p class=orange>Other</p>
<p class="marginleft">
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<z5>Discovery Festival</z5>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Smart spores activation system: inspired by "glow in the dark stick", we designed a sticker with two compartments. One compartment holds the spores, the other the nutrients; when the nutrients compartment is braked, the spores start to germinate, activating the sticker.<br><br>
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<br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We explored the definition of spoiled meat and different ways to test this.<br><br>
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<br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We identified various compounds present in spoiled meat by Gas Chromatography - Mass Spectrometry.<br><br>
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28-29 September 2012, Amsterdam, Eindhoven, Rotterdam - <a href="http://www.discoveryfestival.nl/"><font color=#ff6700>link</font></a>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We made a model which gives insight on how to tweak the growth of <i>Bacillus subtilis</i> based upon literature, experimental data and flux balance analysis.<br>
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Discovery festival is an annual science and arts festival which takes place at the night of 28 September.  
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While before, the festival was only held in Amsterdam, this year it will be extra huge with a festival in
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Eindhoven, Rotterdam and Amsterdam at the same time! Together with iGEM Amsterdam, iGEM Eindhoven and
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iGEM Wageningen we represent iGEM at all three locations. Visitors can experience how synthetic biology works
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themselves in our improvised laboratory, explore the possibilities and can give suggestions for cool synthetic
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biology projects themselves. We are really looking forward to the festival! You can find iGEM Groningen in Nemo in
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Amsterdam together with iGEM Amsterdam. If you want to know how the festival was, look on our facebook page
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(facebook.com/igemgroningen2012) for a report!
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Latest revision as of 00:16, 27 September 2012





Abstract

Every year, one third of global food production -1.3 billion tons of food- is thrown away, partially due to the “best before” dating system. iGEM Groningen 2012 seeks to provide an alternative method of assessing edibility: the Food Warden. It uses an engineered strain of Bacillus subtilis to detect and report volatiles in spoiling meat. The introduced genetic construct uses a promoter to trigger a pigment coding gene. This promoter, identified by microarray analysis, is significantly upregulated in the presence of volatiles from spoiling meat. The activity of the promoter regulates the expression of the pigment reporter and will be visible to the naked eye. For safe usage of the system, spores of our engineered strain are placed into one half of a semi-permeable capsule, the second containing a calibrated amount of nutrients. Breaking the barrier between the two compartments allows germination and growth, thereby activating the spoiling-meat sensor.



Our main accomplishments


In the lab

Most importantly: we developed a construct which makes Bacillus subtilis sense spoiled meat and produce an output in the form of a yellow pigment visible by naked eye.

Design of the “Sticker”: semi-permeable capsule: bacteria are kept inside, volatiles can go through. Proof that Bacillus subtilis grows inside the sticker.

Development of BioBrick psac-cm for Bacillus subtilis.
Easy cloning in Bacillus subtilis in Biobrick fashion for the first time! Advantages: easy to check, BioBrick compatible, E. coli compatible, stable insertion into Bacillus subtilis chromosome.

Identification of spoiled meat sensors by transcriptome analysis. Usage of an innovative experimental setup which can be used to test more sensors in the future.

We made AmilCP and AmilGFP be expressed in Bacillus subtilis. These chromoproteins can be of significant value to the other Bacillus subtilis users in the BioBrick community.


Other

Smart spores activation system: inspired by "glow in the dark stick", we designed a sticker with two compartments. One compartment holds the spores, the other the nutrients; when the nutrients compartment is braked, the spores start to germinate, activating the sticker.

We explored the definition of spoiled meat and different ways to test this.

We identified various compounds present in spoiled meat by Gas Chromatography - Mass Spectrometry.

We made a model which gives insight on how to tweak the growth of Bacillus subtilis based upon literature, experimental data and flux balance analysis.


Our sponsors:

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