Team:Groningen/DesignTest2

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<z1>Presentations</z1><br>
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<z1>Abstract</z1>
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<z2>Upcoming Presentations</z3>
 
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<z3>Dutch iGEM symposium</z3> – Wednesday 3 October – Eindhoven – <a href="https://2012.igem.org/Team:TU-Eindhoven" target=_blank"><FONT COLOR=#ff6700>link</FONT></a>
 
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Wednesday 3 October all Dutch iGEM teams are invited to present their projects. Interested students, researchers and family/friends are invited!
 
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<z3>ESF EuroSYNBio conference</z3> - Thursday 4 October – Het Kasteel, Groningen – <a href="http://www.esf.org/activities/eurocores/running-programmes/eurosynbio/events.html" target=_blank"><FONT COLOR=#ff6700>link</FONT></a>
 
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<br>
 
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EuroSYNBio is a programme of the European Science Foundation. It aims not only to stimulate research in the field of synthetic biology, but also discusses the role of synthetic biology in society.
 
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<z2>Previous presentations</z2>
 
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<z3>Transnatural Conference</z3>  - Tuesday 18 September - NEMO, Amsterdam – <A HREF="www.transnatural.nl" TARGET="_blank"><FONT COLOR=#ff6700>link</FONT></A>
 
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Every year, one third of global food production -1.3 billion tons of food- is thrown away, partially due to the “best before” dating system.
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<z7>iGEM Groningen 2012</z7> seeks to provide an alternative method of assessing edibility: the <z7>Food Warden</z7>. It uses an <z7>engineered strain</z7>
 +
of <i>Bacillus subtilis</i> to detect and report volatiles in spoiling meat. The introduced <z7>genetic construct</z7> uses a promoter to trigger
 +
a pigment coding gene. This promoter, <z7>identified by microarray analysis</z7>, is significantly upregulated in the presence of
 +
<z7>volatiles from spoiling meat</z7>. The activity of the <z7>promoter</z7> regulates the expression of the <z7>pigment reporter</z7> and will
 +
be visible to the naked eye. For safe usage of the system, spores of our engineered strain are placed into one half of a semi-permeable
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<z7>capsule</z7>, the second containing a calibrated amount of nutrients. Breaking the barrier between the two compartments allows
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<z7>germination and growth</z7>, thereby activating the <z7>spoiling-meat sensor</z7>.
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On Tuesday 18 September we managed to get out of the lab and into the Science Exhibition Centre Nemo.
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<br>
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It was a great learning experience to present for an audience without a scientific background.
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<div class="cte2">
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<div class="ctd2">
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<a name="MainAcc"></a><z2 >Our main accomplishments</z2><br>
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<z3>GBB symposium</z3> – Thursday 6 September – Hampshire Hotel, Groningen – <A HREF="http://www.rug.nl/fmns-research/gbb/education/generalcourses/gbbsymposium/index" TARGET="_blank"><FONT COLOR=#ff6700>link</FONT></A>
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<br><br>
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At the annual conference of the Groningen Biomolecular Science and Biotechnology institute, we got the chance to give a lecture about our project
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<p class=orange>In the lab</p>
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and present our poster.
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<p class="marginleft">
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<br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Most importantly: we developed a construct which makes <i>Bacillus subtilis</i> sense spoiled meat and produce an output in the form of a yellow pigment visible by naked eye.<br><br>
-
<br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Design of the “<a href="https://2012.igem.org/Team:Groningen/Sticker">Sticker</a>”: semi-permeable capsule: bacteria are kept inside, volatiles can go through. Proof that <i>Bacillus subtilis</i> grows inside the sticker.<br><br>
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<z3>Dutch iGEM mini symposium</z3> – Monday 9 July – Wageningen – <A HREF="https://2012.igem.org/Team:Wageningen_UR/miniSymposium" TARGET="_blank"><FONT COLOR=#ff6700>link</FONT></A>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Development of BioBrick psac-cm for <i>Bacillus subtilis</i>. <br><a href="http://partsregistry.org/Part:BBa_K818000" target=_blank><font size=4 color=#FF6700><b>Easy cloning in <i>Bacillus subtilis</i> in Biobrick fashion for the first time!</b></font></a> Advantages: easy to check, BioBrick compatible, <i>E. coli</i> compatible, stable insertion into <i>Bacillus subtilis</i> chromosome.<br><br>
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<br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Identification of spoiled meat sensors by transcriptome analysis. Usage of an innovative experimental setup which can be used to test more sensors in the future.<br><br>
-
<br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We made AmilCP and AmilGFP be expressed in <i>Bacillus subtilis</i>. These chromoproteins can be of significant value to the other <i>Bacillus subtilis</i> users in the BioBrick community.<br><br><br>
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On the 10th of July all Dutch iGEM teams met in Wageningen for the first mini-symposium (Greek for meeting and having a drink together).  
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</p>
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We shared our ideas and presented our work.
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<p class=orange>Other</p>
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See the video <A HREF="http://wurtv.wur.nl/P2GTV/cataloguepage.aspx" TARGET="_blank"><FONT COLOR=#ff6700>Symposium iGEM Netherlands 2</FONT></A> (posted on July 9 2012).  
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<p class="marginleft">
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The iGEM Groningen presentation starts at time point 1:42.
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Smart spores activation system: inspired by "glow in the dark stick", we designed a sticker with two compartments. One compartment holds the spores, the other the nutrients; when the nutrients compartment is braked, the spores start to germinate, activating the sticker.<br><br>
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<br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We explored the definition of spoiled meat and different ways to test this.<br><br>
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<br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We identified various compounds present in spoiled meat by Gas Chromatography - Mass Spectrometry.<br><br>
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After these interesting talks, a nice dinner was served: we had a BBQ inside the chemistry lab of an old school building. Very suitable for us lab rats!
+
<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We made a model which gives insight on how to tweak the growth of <i>Bacillus subtilis</i> based upon literature, experimental data and flux balance analysis.<br>
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<br>
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<br>
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<z3>Biomedical Engineering Symposium</z3> – Wednesday 13 June – UMCG, Groningen – <A HREF="http://www.biomedische-technologie.nl/" TARGET="_blank"><FONT COLOR=#ff6700>link</FONT></A>
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<br>
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<br>
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The study society of Biomedical Engineering annually organizes a conference for students and researchers at the University Medical Centre of Groningen.
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This year, the theme was “Bright Ideas”.
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Latest revision as of 00:16, 27 September 2012





Abstract

Every year, one third of global food production -1.3 billion tons of food- is thrown away, partially due to the “best before” dating system. iGEM Groningen 2012 seeks to provide an alternative method of assessing edibility: the Food Warden. It uses an engineered strain of Bacillus subtilis to detect and report volatiles in spoiling meat. The introduced genetic construct uses a promoter to trigger a pigment coding gene. This promoter, identified by microarray analysis, is significantly upregulated in the presence of volatiles from spoiling meat. The activity of the promoter regulates the expression of the pigment reporter and will be visible to the naked eye. For safe usage of the system, spores of our engineered strain are placed into one half of a semi-permeable capsule, the second containing a calibrated amount of nutrients. Breaking the barrier between the two compartments allows germination and growth, thereby activating the spoiling-meat sensor.



Our main accomplishments


In the lab

Most importantly: we developed a construct which makes Bacillus subtilis sense spoiled meat and produce an output in the form of a yellow pigment visible by naked eye.

Design of the “Sticker”: semi-permeable capsule: bacteria are kept inside, volatiles can go through. Proof that Bacillus subtilis grows inside the sticker.

Development of BioBrick psac-cm for Bacillus subtilis.
Easy cloning in Bacillus subtilis in Biobrick fashion for the first time! Advantages: easy to check, BioBrick compatible, E. coli compatible, stable insertion into Bacillus subtilis chromosome.

Identification of spoiled meat sensors by transcriptome analysis. Usage of an innovative experimental setup which can be used to test more sensors in the future.

We made AmilCP and AmilGFP be expressed in Bacillus subtilis. These chromoproteins can be of significant value to the other Bacillus subtilis users in the BioBrick community.


Other

Smart spores activation system: inspired by "glow in the dark stick", we designed a sticker with two compartments. One compartment holds the spores, the other the nutrients; when the nutrients compartment is braked, the spores start to germinate, activating the sticker.

We explored the definition of spoiled meat and different ways to test this.

We identified various compounds present in spoiled meat by Gas Chromatography - Mass Spectrometry.

We made a model which gives insight on how to tweak the growth of Bacillus subtilis based upon literature, experimental data and flux balance analysis.


Our sponsors:

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