Team:Goettingen/Notebook/Results deu

From 2012.igem.org

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<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" dir="ltr" lang="en"><head>
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{{GoettingenHeader|deu=Team:Goettingen/Notebook/Results_deu|eng=Team:Goettingen/Notebook/Results}}
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__NOTOC__
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                                <meta name="keywords" content="Team:Goettingen,Team:Goettingen,Team:Goettingen/Homing coli,Team:Goettingen/Human Practice/Flash coli,Team:Goettingen/Press,Team:Goettingen/Project/General information">
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== Homing coli: <i>E. coli</i> zum Spürhund ausbilden ==
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/*...............................................................................................*/
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Sprache: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> <a href="https://2012.igem.org/Team:Goettingen/Notebook/Summary">English</a>, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> Deutsch <br>
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Der Modellorganismus <i>Escherichia coli</i> ist natürlicherweise dazu in der Lage, Substanzen in seiner Umgebung wahrzunehmen
Der Modellorganismus <i>Escherichia coli</i> ist natürlicherweise dazu in der Lage, Substanzen in seiner Umgebung wahrzunehmen
und sich gerichtet dorthin zu bewegen. Dieses Phänomen nennt man Chemotaxis. Hier wenden wir direkte Mutagenese der Chemorezeptoren an,
und sich gerichtet dorthin zu bewegen. Dieses Phänomen nennt man Chemotaxis. Hier wenden wir direkte Mutagenese der Chemorezeptoren an,
Line 541: Line 11:
Ist <i>E. coli</i> schließlich als Spürhund etabliert, sind die Möglichkeiten für Anwendungsbereiche primär in der Medizin,
Ist <i>E. coli</i> schließlich als Spürhund etabliert, sind die Möglichkeiten für Anwendungsbereiche primär in der Medizin,
aber auch in anderen Zusammenhängen, nahezu endlos.
aber auch in anderen Zusammenhängen, nahezu endlos.
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== #1 - Selektion / (bakterielles) Schwimmen ==
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[[File:Goett_Coli_select.jpg]]
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== #2 - Verbesserung der Geschwindigkeit  ==
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[[File:Goett_Coli_speed.jpg]]
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== #3 - Chemorezeptoren ==
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[[File:Goett_Coli_nose.jpg]]
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Latest revision as of 15:09, 21 September 2012

Deutsch  / English 

Homing coli: E. coli zum Spürhund ausbilden

Der Modellorganismus Escherichia coli ist natürlicherweise dazu in der Lage, Substanzen in seiner Umgebung wahrzunehmen und sich gerichtet dorthin zu bewegen. Dieses Phänomen nennt man Chemotaxis. Hier wenden wir direkte Mutagenese der Chemorezeptoren an, um die Detektion von neuen Substanzen durch die Modifikation von fünf individuellen Aminosäureresten in der Ligandenbindestelle zu ermöglichen. Wir haben eine effektive Selektionsmethode mit speziellen "Schwimmplatten" entwickelt, um die gerichtete Bewegung zu einer Substanz zu untersuchen. Zusätzlich wird E. coli auf erhöhte Geschwindigkeit konditioniert, wobei das bakterielle Flagellum in den Fokus gerückt ist. Auf der Basis dieses Selektionssystems werden mutierte Varianten aus der Mutationsbibliothek identifiziert. Auf diese Weise haben wir uns zum Ziel gesetzt, E. coli darauf zu trainieren, neue Moleküle wie Tumormarker zu detektieren. Ist E. coli schließlich als Spürhund etabliert, sind die Möglichkeiten für Anwendungsbereiche primär in der Medizin, aber auch in anderen Zusammenhängen, nahezu endlos.

#1 - Selektion / (bakterielles) Schwimmen

Goett Coli select.jpg

#2 - Verbesserung der Geschwindigkeit

Goett Coli speed.jpg

#3 - Chemorezeptoren

Goett Coli nose.jpg


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