Team:Groningen/Notebook/Wetwork 31August2012

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Ligation of AmilGFP and AmilACP into the Psac containing Fnr and s-box. Digestion for the AmilGFP and AmilACP will be done with PstI and XbaI. Plasmid with promotor will be cut with EcoRi and SpeI.
 
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Ligation of AmilGFP and AmilACP into the Psac containing Fnr and s-box. Digestion for the AmilGFP and AmilACP will be done with PstI and XbaI. Plasmid with promotor will be cut with EcoRi and SpeI.<br>
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Elbrich<br>
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Plated out E.coli with LMU Munich biobricks showed white and purple/reddish colonies.<br>
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Yonathan<br>
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Prepared digests for the construction of psac_cmt+pLacI+amilCP and psac_cmt+pLacI+amilGFP again for future use.<br>
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Plated out E.coli with LMU Munich biobricks showed white and purple/reddish colonies.
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Latest revision as of 18:55, 26 September 2012







Tom/Arjan

Ligation of AmilGFP and AmilACP into the Psac containing Fnr and s-box. Digestion for the AmilGFP and AmilACP will be done with PstI and XbaI. Plasmid with promotor will be cut with EcoRi and SpeI.


Elbrich

Plated out E.coli with LMU Munich biobricks showed white and purple/reddish colonies.


Yonathan

Prepared digests for the construction of psac_cmt+pLacI+amilCP and psac_cmt+pLacI+amilGFP again for future use.


Back to notebook



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