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Team:Groningen/Notebook/Wetwork 11June2012
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Nisa: designed primers for construct with pigments (lycopene, violacein) from Cambrigde 2008. Further busy with design/literature study of positive feedback loop. <br><br> | Nisa: designed primers for construct with pigments (lycopene, violacein) from Cambrigde 2008. Further busy with design/literature study of positive feedback loop. <br><br> | ||
Literature study: search for promoter.<br><br> | Literature study: search for promoter.<br><br> | ||
| + | Transformed Violacein (BBa_K274002) into e.coli dh5a: <br> | ||
| + | *2 uL of DNA into 50-75 uL of cells<br> | ||
| + | *30 min on ice <br> | ||
| + | *2 min, 42 degrees C <br> | ||
| + | *back on ice, add 300 uL LB <br> | ||
| + | *1.5 hour in 37 degrees C <br> | ||
| + | *plate (LB with 10 ug/mL chloramphenicol), O/N at 37 degrees C | ||
| + | |||
<br> | <br> | ||
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
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{{Template:SponsorsGroningen2012}} | {{Template:SponsorsGroningen2012}} | ||
Latest revision as of 16:38, 25 September 2012
Nisa: designed primers for construct with pigments (lycopene, violacein) from Cambrigde 2008. Further busy with design/literature study of positive feedback loop.
Literature study: search for promoter.
Transformed Violacein (BBa_K274002) into e.coli dh5a:
*2 uL of DNA into 50-75 uL of cells
*30 min on ice
*2 min, 42 degrees C
*back on ice, add 300 uL LB
*1.5 hour in 37 degrees C
*plate (LB with 10 ug/mL chloramphenicol), O/N at 37 degrees C
Back to notebook
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