Team:Groningen

From 2012.igem.org

(Difference between revisions)
Line 58: Line 58:
<div style="position:absolute; left: 0px; top: 380px;opacity:0.4; filter:alpha(opacity=40);z-index:-1;">
<div style="position:absolute; left: 0px; top: 380px;opacity:0.4; filter:alpha(opacity=40);z-index:-1;">
-
<img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150" height="150">
+
<img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="200" height="200">
</div>
</div>
-
<div style="position:absolute; left: 870px; top: 580px;opacity:0.4; filter:alpha(opacity=40); z-index:-1;">
+
<div style="position:absolute; left: 820px; top: 530px;opacity:0.4; filter:alpha(opacity=40); z-index:-1;">
-
<img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150" height="150">
+
<img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="200" height="200">
</div>
</div>
-
<div style="position:absolute; left: 0px; top: 700px;opacity:0.4; filter:alpha(opacity=40); z-index:-1;">
+
<div style="position:absolute; left: 0px; top: 680px;opacity:0.4; filter:alpha(opacity=40); z-index:-1;">
-
<img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150" height="150">
+
<img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="200" height="200">
</div>
</div>
Line 89: Line 89:
margin-bottom:20px;
margin-bottom:20px;
margin-right:0px;
margin-right:0px;
-
margin-left:160px;
+
margin-left:210px;
-
margin-right:10px;
+
margin-right:100px;
Line 109: Line 109:
color:white;  
color:white;  
-
margin-top:50px;
+
margin-top:0px;
-
margin-bottom:20px;
+
margin-bottom:38px;
margin-right:0px;
margin-right:0px;
-
margin-left:10px;
+
margin-left:100px;
-
margin-right:160px;
+
margin-right:210px;
 +
}
 +
p.marginleftheader
 +
{
 +
font-size:14pt;
 +
line-height:14pt;
 +
color:white;
 +
 
 +
margin-top:38px;
 +
margin-bottom:0px;
 +
margin-left:650px;
 +
margin-right:210px;
}
}
</style>
</style>
Line 120: Line 131:
<body>
<body>
-
<p class="margin"><b>The Food Warden: it's rotten and you know it!</b><br>
+
<p class="margin"><A HREF="https://2012.igem.org/Team:Groningen/Stop_the_food_waste_initiative" TARGET="_blank"><FONT COLOR=#ff6700><b>Stop the food waste!</b></FONT></A><br><br>
Every year, 1,300,000,000 tons of food are thrown away worldwide. This is one third of the global food production. One of the reasons for this is the fact that best before dates are imprecise. A reliable way of monitoring whether food is spoiled or not could save up to 600 euro per household per year.
Every year, 1,300,000,000 tons of food are thrown away worldwide. This is one third of the global food production. One of the reasons for this is the fact that best before dates are imprecise. A reliable way of monitoring whether food is spoiled or not could save up to 600 euro per household per year.
</p>
</p>
-
<p class="marginleft">
+
<p class="marginleftheader">
-
Our goal is to build a system to sense meat spoilage. When a package of meat is saved after opening, a closed sticker containing <i>Bacillus subtilis</i> spores is activated by breaking the middle compartment, similar to activating a glow-in-the-dark stick, whereby alanine and water are mixed with the spores to cause germination. When the germinated microbes come in contact with volatiles from rotting meat, a pathway is activated which results to the production of a pigment. When this happens, the consumer knows that he has to throw away the food. <br>
+
<A HREF="https://2012.igem.org/Team:Groningen/Project" TARGET="_blank"><FONT COLOR=#ff6700><b>The Food Warden</b></FONT></A></p>
-
<br>
+
<p class="marginleft"><br>
 +
The Food Warden is a system which detects meat spoilage. It consists of <i>Bacillus subtilis</i> cells. Their natural genetic response to the rotting gases of meat has been identified and linked to a pigment production system. In this way, a consumer can easily see when the meat is spoiled: it's rotten and you know it! <A HREF="https://2012.igem.org/Team:Groningen/Project" TARGET="_blank"><FONT COLOR=#ff6700>read more</FONT></A>
</p>
</p>
<p class="margin">
<p class="margin">
-
We use two strategies to make <i>Bacillus subtilis</i> react to rotten meat volatiles. The first involves TnrA, a transcription regulator involved in nitrogen metabolism. The picture below summerizes this goal pathway. The other strategy comprises the identification of new promoters reacting to rotten meat volatiles. In both cases, the promoter will be combined to a pigment production pathway. Besides that, iGEM Groningen 2012 aims to identify the volatiles that cause the promoter induction by using Gas Chromatography and Mass Spectrometry. A detailed description of our project plan can be found <A HREF="https://2012.igem.org/Team:Groningen/Project" TARGET="_blank"><FONT COLOR=#ff6700>on the project page</FONT></A>
+
<A HREF="https://2012.igem.org/Team:Groningen/Wetwork" TARGET="_blank"><FONT COLOR=#ff6700><b>Now in the lab</b></FONT></A>
 +
<br><br>
 +
With the iGEM deadline being just four weeks away, the lab is now always occupied in order to make the Food Warden work. The newly identified genes in the running to become our PBADmeat promoter are ready to be tested, the pigments are waiting to be transformed. But <i>Bacillus</i> biobricks are tricky things... <A HREF="https://2012.igem.org/Team:Groningen/Wetwork" TARGET="_blank"><FONT COLOR=#ff6700>read more</FONT></A>
 +
<br>
 +
<br>
 +
<br>
</p>
</p>

Revision as of 16:42, 29 August 2012




Foodwarden



Stop the food waste!

Every year, 1,300,000,000 tons of food are thrown away worldwide. This is one third of the global food production. One of the reasons for this is the fact that best before dates are imprecise. A reliable way of monitoring whether food is spoiled or not could save up to 600 euro per household per year.

The Food Warden


The Food Warden is a system which detects meat spoilage. It consists of Bacillus subtilis cells. Their natural genetic response to the rotting gases of meat has been identified and linked to a pigment production system. In this way, a consumer can easily see when the meat is spoiled: it's rotten and you know it! read more

Now in the lab

With the iGEM deadline being just four weeks away, the lab is now always occupied in order to make the Food Warden work. The newly identified genes in the running to become our PBADmeat promoter are ready to be tested, the pigments are waiting to be transformed. But Bacillus biobricks are tricky things... read more



Our sponsors:

Retrieved from "http://2012.igem.org/Team:Groningen"