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Team:Groningen/Notebook/Wetwork 19July2012
From 2012.igem.org
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Empty backbone will form red colonies, while colony with insert will form white ones | Empty backbone will form red colonies, while colony with insert will form white ones | ||
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| + | Renske | ||
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| + | Bought minced meat (30% beef, 70% pork), made aliquots of 1 g, 5 g and 10 g, frozen all to 20 degrees Celsius. | ||
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| + | Poured 100 plates with Trypton Soya Agar. | ||
Revision as of 17:55, 2 August 2012
Tom
Growth curves of Bacillus in SMM at different temperatures: 37 °C, 26 °C, 18°C and 5 °C and different starting masses: OD = 0.1 and OD = 0.3.
Nisa
Overnight culture E.coli DH5a LB+ 15ug/ml Cm
->pSB1C3 ->backbone for E.coli
Empty backbone will form red colonies, while colony with insert will form white ones
Renske
Bought minced meat (30% beef, 70% pork), made aliquots of 1 g, 5 g and 10 g, frozen all to 20 degrees Celsius.
Poured 100 plates with Trypton Soya Agar.
