Team:Groningen

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|'''Bacillus salus carnis'''
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<br>
<br>
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B. salus carnis will be engineered strain of b. subtilis which provides a real-time edibility indicator for packaged meat and fish.
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<div class="cte">
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<br>  
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<div class="ctd">
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<z1 >Food Warden</z1><br>
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</div>
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</div>
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</head>
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<br><br><br>
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<table class=Inside>
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<td class=Left>
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<p class="marginTable">
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<img src="https://static.igem.org/mediawiki/2012/9/97/Groningen2012_AD20121915_Sticker.png" width="130" height="130" align=left style="padding-right: 10px; padding-top: 20px; padding-bottom:15px;"><a href="https://2012.igem.org/Team:Groningen/Project"><font color=#ff6700 size=4><b>The Food Warden</b></font></a><br>
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The Food Warden is a system which detects meat spoilage. It uses <i>Bacillus subtilis</i> cells. Their natural genetic response to the gases of rotten meat has been identified and linked to a pigment production system. In this way, a consumer can easily see when the meat is spoiled: <b><i>it's rotten and you know it!</i></b><br>
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<a href="https://2012.igem.org/Team:Groningen/Project"><font color=#ff6700 size=2>read more</font></a><br>
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</td>
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</tr>
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<table class=Inside>
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<td class=Left>
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<p class="marginTable">
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<a href="https://2012.igem.org/Team:Groningen/Data_page"><font color=#ff6700 size=4><b>How does it work?</b></font></a><br>
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Volatiles from rotten meat activate the P<i><font size=2>sboA</font></i> promoter, triggering the production of pigments (blue or yellow).<br>More details can be found on our <a href=https://2012.igem.org/Team:Groningen/Data_page><font color=#ff6700><b>Data Page</b></font></a>.<br></p>
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<table class=Inside>
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<td class=Left>
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<p class="marginTable">
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<a href="https://2012.igem.org/Team:Groningen/Stop_the_food_waste_initiative"><font color=#ff6700 size=4><b>Stop the food waste!</b></font></a><br>
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<img src="https://static.igem.org/mediawiki/2012/f/f2/Groningen2012_AD20120915_StopFoodWaste.png" width="130" height="130" align=right style="padding-left: 5px; padding-right: 5px;">Every year, 1.3 billion tons of food are thrown away worldwide (one third of total food production), partly due to the fact that best before dates are imprecise. A reliable way of monitoring whether food is spoiled or not could save money and reduce food waste.<br>
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<a href="https://2012.igem.org/Team:Groningen/Stop_the_food_waste_initiative"><font color=#ff6700 SIZE=2pt>read more</font></a><br><br><br>
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<td style="vertical-align:top;">
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<table class=Inside>
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<td class=Right>
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<p class=vid>
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<iframe src="http://player.vimeo.com/video/52145107?title=0&amp;byline=0&amp;portrait=0&amp;badge=0&amp;color=a93434&amp;autoplay=1" width="300" height="225" frameborder="0"></iframe></p>
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</td>
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<table class=Inside>
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<td class=Right>
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<p class="marginAcc" align=center>
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<font size = 4 color=#ff6700><b>Our achievements</b></font><br><br>
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<img src=https://static.igem.org/mediawiki/2012/1/16/Groningen2012_GoldMedal.png style="padding-bottom:3px;"><br><font color=#ffff00><b> Gold Medal</b></font><br><br>
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<img src="https://static.igem.org/mediawiki/2012/2/27/Groningen2012_ADTrophy_igem.png"><br>
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<b>Regional Winner</b><br>
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<font size=2>at the iGEM European Jamboree</font><br></p>
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<p class="marginAcc" align=center>
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<img src="https://static.igem.org/mediawiki/2012/c/c5/IGGroningen2012_Brick.png" height=50><br>
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<b>World Championship!!!</b><br>
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</td>
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</table>
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<table class=Bottom>
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<p align = center>
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<a href="https://2012.igem.org/files/presentation/Groningen_Championship.pdf" target=_blank>
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Our presentation [.pdf]</a> ||
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<a href="https://2012.igem.org/files/video/Groningen_Championship.mp4" target=_blank>
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Our presentation (recording)</a> ||
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<a href="https://igem.org/images/easel_icon.png" target=_blank>
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Our poster</a>
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</p>
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</td>
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</table>
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<a name="#"></a><br>
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<div class="cte3">
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<div class="ctd3">
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<z3 >Picture gallery</z3><br>
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</div>
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</div>
<br>
<br>
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It uses a modified carotenoid pathway to indicate (Fig. h1):
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<ul class="hoverbox">
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# Toxic concentrations of ammonium
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<li>
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# Toxicity due to pH
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<a href="#"><img src="https://static.igem.org/mediawiki/2012/e/ee/Groningen2012_ADGallery1.png" width=125 height=125 /><img src="https://static.igem.org/mediawiki/2012/6/65/Groningen2012_ADGallery1prev.png" class="preview" width=250 height=280 /></a>
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|[[File:JP 20120504 ReactionPathwayOverview v03.png|680px|center]]
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{|align="center"
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|Fig. h1. Overview of the functional reaction pathway for b. salus carnis.
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<a href="#"><img src="https://static.igem.org/mediawiki/2012/6/69/Groningen2012_ADGallery13.png" width=125 height=125 /><img src="https://static.igem.org/mediawiki/2012/6/61/Groningen2012_ADGallery13prev.png" class="preview" width=250 height=280 /></a>
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<a href="#"><img src="https://static.igem.org/mediawiki/2012/7/7d/Groningen2012_ADGalleryWinners.png" width=250 height=260 /><img src="https://static.igem.org/mediawiki/2012/e/e7/Groningen2012_ADGalleryWinners_prev.png" class="preview" width=325 height=355 /></a>
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<a href="#"><img src="https://static.igem.org/mediawiki/2012/e/e3/Groningen2012_ADGallery8.png" width=260 height=125 /><img src="https://static.igem.org/mediawiki/2012/8/8a/Groningen2012_ADGallery8prev.png" class="preview" width=420 height=231 /></a>
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<a href="#"><img src="https://static.igem.org/mediawiki/2012/0/0c/Groningen2012_ADGallery9.png" width=125 height=125 /><img src="https://static.igem.org/mediawiki/2012/5/5a/Groningen2012_ADGallery9prev.png" class="preview" width=250 height=280 /></a>
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<a href="#"><img src="https://static.igem.org/mediawiki/2012/f/f4/Groningen2012_ADGallery22.png" width=125 height=125 /><img src="https://static.igem.org/mediawiki/2012/d/dc/Groningen2012_ADGallery22prev.png" class="preview" width=250 height=280 /></a>
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<a href="#"><img src="https://static.igem.org/mediawiki/2012/0/04/Groningen2012_ADGallery14.png" width=125 height=125 /><img src="https://static.igem.org/mediawiki/2012/2/20/Groningen2012_ADGallery14prev.png" class="preview" width=250 height=280 /></a>
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<a href="#"><img src="https://static.igem.org/mediawiki/2012/c/ce/Groningen2012_ADGallery21.png" width=250 height=125 /><img src="https://static.igem.org/mediawiki/2012/a/a9/Groningen2012_ADGallery21prev.png" class="preview" width=420 height=231 /></a>
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<a href="#"><img src="https://static.igem.org/mediawiki/2012/a/a6/Groningen2012_ADGallery18.png" width=125 height=125 /><img src="https://static.igem.org/mediawiki/2012/0/0b/Groningen2012_ADGallery18prev.png" class="preview" width=250 height=280 /></a>
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<a href="#"><img src="https://static.igem.org/mediawiki/2012/e/ec/Groningen2012_ADGallery3.png" width=125 height=125 /><img src="https://static.igem.org/mediawiki/2012/c/cb/Groningen2012_ADGallery3prev.png" class="preview" width=250 height=280 /></a>
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<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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<br><br><br><br><br><br><br><br><br><br><br>
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<div style="position: relative; top: -100px;">
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<div class="cte2">
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<div class="ctd2">
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<z2 >Gold medal checklist</z2><br>
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</div>
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</div>
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<p align=center><font color=white size=2>Click on any of the list below to see the appropriate wiki section!</font></p>
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<p class="marginChecklist"><b>BRONZE</b>
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<br>
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<br>
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<a href="https://igem.org/Team.cgi" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Team registration done</a><br>
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<a href="https://igem.org/2012_Judging_Form?id=818" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Judging form completed</a><br>
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<a href="https://2012.igem.org/Team:Groningen" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Beautiful Team Wiki finished</a><br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> We present our poster and project at iGEM Jamboree in Amsterdam at Saturday 6 October 2012<br>
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<a href="https://2012.igem.org/Team:Groningen/OurBiobrick" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Our Biobricks</a>
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<b>SILVER</b> (in addition to bronze medal requirements)
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<a href="http://partsregistry.org/Part:BBa_K818000" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Proof that our BioBrick works!</a><br>
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> <a href="http://partsregistry.org/Part:BBa_K818600" target=_blank>Characterization of our BioBrick's operation: BBa_K818600</a>
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<b>GOLD</b> (in addition to silver and bronze medal requirements, any one or more of the following)
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<img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> We improved the function of existing BioBricks: <a href="http://partsregistry.org/Part:BBa_K592010" target=_blank>BBa_K592010</a>, <a href="http://partsregistry.org/Part:BBa_K592009" target=_blank>BBa_K592009</a><br>
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<a href="https://2012.igem.org/Team:Groningen/international_cooperation#LMUMunich" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> We helped iGEM LMU Munich by characterizing their parts</a><br>
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<a href="https://2012.igem.org/Team:Groningen/Safety" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> We explored the topic of safety for GMOs that would be used near food</a><br>
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<a href="https://2012.igem.org/Team:Groningen/Stop_the_food_waste_initiative" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> We raised public awareness about food waste in developed countries</a><br>
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<a href="https://2012.igem.org/Team:Groningen/market_research" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> We surveyed the broad public about the need for Food Warden product and GMOs safety</a>
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Latest revision as of 14:21, 1 September 2013





Food Warden



The Food Warden
The Food Warden is a system which detects meat spoilage. It uses Bacillus subtilis cells. Their natural genetic response to the gases of rotten meat has been identified and linked to a pigment production system. In this way, a consumer can easily see when the meat is spoiled: it's rotten and you know it!
read more

How does it work?
Volatiles from rotten meat activate the PsboA promoter, triggering the production of pigments (blue or yellow).
More details can be found on our Data Page.

Stop the food waste!
Every year, 1.3 billion tons of food are thrown away worldwide (one third of total food production), partly due to the fact that best before dates are imprecise. A reliable way of monitoring whether food is spoiled or not could save money and reduce food waste.
read more

Our achievements


Gold Medal


Regional Winner
at the iGEM European Jamboree


World Championship!!!

Our presentation [.pdf] || Our presentation (recording) || Our poster


Picture gallery





































Gold medal checklist

Click on any of the list below to see the appropriate wiki section!

BRONZE

Team registration done
Judging form completed
Beautiful Team Wiki finished
We present our poster and project at iGEM Jamboree in Amsterdam at Saturday 6 October 2012
Our Biobricks

SILVER (in addition to bronze medal requirements)

Proof that our BioBrick works!
Characterization of our BioBrick's operation: BBa_K818600

GOLD (in addition to silver and bronze medal requirements, any one or more of the following)

We improved the function of existing BioBricks: BBa_K592010, BBa_K592009
We helped iGEM LMU Munich by characterizing their parts
We explored the topic of safety for GMOs that would be used near food
We raised public awareness about food waste in developed countries
We surveyed the broad public about the need for Food Warden product and GMOs safety

Our sponsors:

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