Team:Groningen/Project
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<z7>capsule</z7>, the second containing a calibrated amount of nutrients. Breaking the barrier between the two compartments allows | <z7>capsule</z7>, the second containing a calibrated amount of nutrients. Breaking the barrier between the two compartments allows | ||
<z7>germination and growth</z7>, thereby activating the <z7>spoiling-meat sensor</z7>. | <z7>germination and growth</z7>, thereby activating the <z7>spoiling-meat sensor</z7>. | ||
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- | <a | + | <div class="cte3"> |
- | </ | + | <div class="ctd3"> |
- | </ | + | <z2 >Completed after European Regionals</z2><br><br> |
+ | </div> | ||
+ | </div> | ||
+ | <p class="marginChecklist"> | ||
+ | <br> | ||
+ | <br> | ||
+ | <a href="https://2012.igem.org/Team:Groningen/in_development" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Tested a construct with downregulated promoter <i>WapA</i></a><br> | ||
+ | <a href="https://2012.igem.org/Team:Groningen/Construct" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Performed enhanced construct characterization</a><br> | ||
+ | <a href="https://2012.igem.org/Team:Groningen/Sticker" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Characterized the influence of oxygen on germination and growth within the sticker</a><br> | ||
+ | <a href="https://2012.igem.org/Team:Groningen/market_research" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Expanded the scope of the market survey</a><br> | ||
+ | <a href="https://2012.igem.org/Team:Groningen/international_cooperation" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png"> Collaborated with Cambridge 2012</a><br> | ||
+ | <br> | ||
+ | </p> | ||
- | + | <a name="MainAcc"></a> | |
- | + | <div class="cte2"> | |
- | + | <div class="ctd2"> | |
- | + | <a name="MainAcc"></a><z2 >Our main accomplishments</z2><br><br> | |
- | + | </div> | |
- | + | </div> | |
- | <a | + | <br> |
- | < | + | <br> |
- | + | <p align=center style="color: white; font-size: 10pt;"> | |
- | + | <i>Hover your mouse over the pictures to see more!</i> | |
- | + | </p> | |
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+ | <ul class="hoverbox"> | ||
+ | <li> | ||
+ | <a href="#"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/c/cb/Groningen2012_RR1capsule_break.png" width=200 /> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/a/ac/Groningen2012_ADStickerBig.png" class="preview" width=400 /> | ||
+ | </a> | ||
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+ | <td class="accPic"> | ||
+ | <ul class="hoverbox"> | ||
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+ | <a href="#"><img src="https://static.igem.org/mediawiki/2012/c/c2/Groningen2012_RR_20120909_rotting.png" width=150 /><img src="https://static.igem.org/mediawiki/2012/7/74/Groningen2012_ADVolatilesBig.png" class="preview" width=400 /></a> | ||
+ | </li> | ||
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+ | <td class="accCap"> | ||
+ | <p class="small"> | ||
+ | <font color=#FF6700><br><b>1.</b></font> We designed and tested the "<a class="inlink" href="https://2012.igem.org/Team:Groningen/Sticker">Sticker</a>": a capsule in which bacteria are kept inside and volatiles can go through. We used a model to get insight on how to tweak the growth of <i>Bacillus subtilis</i>. | ||
+ | </p> | ||
+ | </td> | ||
+ | <td class="accCap"> | ||
+ | <p class="small"> | ||
+ | <font color=#FF6700><b>2.</b></font> We explored the definition of spoiled meat and ways to check meat spoilage. We identified <a class="inlink" href="https://2012.igem.org/Team:Groningen/volatiles">various compounds</a> present in spoiled meat.<br> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="accPic"> | ||
+ | <ul class="hoverbox"> | ||
+ | <li> | ||
+ | <a href="#"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/2/29/Groningen2012_RR20120909_badmeat.png" width=200 /> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/e/ec/Groningen2012_ADSensorBig.png" class="preview" width=400 /> | ||
+ | </a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | <td class="accPic"> | ||
+ | <ul class="hoverbox"> | ||
+ | <li> | ||
+ | <a href="#"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/2/24/Groningen2012_RRADPsac_transp.png" width=150 /> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/c/c1/Groningen2012_AdBackboneBig.png" class="preview" width=400 /> | ||
+ | </a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="accCap"> | ||
+ | <p class="small"> | ||
+ | <font color=#FF6700><b>3.</b></font> We identified spoiled meat <a class="inlink" href="https://2012.igem.org/Team:Groningen/Sensor">sensors</a> by transcriptome analysis.<br><br><br> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td class="accCap"> | ||
+ | <p class="small"> | ||
+ | <font color=#FF6700><b>4.</b></font> <a class="inlink" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K818000" target="_blank">Backbone pSac-Cm</a>: easy cloning in<br><i>B. subtilis</i>, the BioBrick way, for the first time! It's easy to check, BioBrick compatible, <i>E. coli</i> compatible, stabily inserted into the <i>B. subtilis</i> chromosome. | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="accPic"> | ||
+ | <ul class="hoverbox"> | ||
+ | <li> | ||
+ | <a href="#"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/f/f1/Groningen2012_RRADPigments_transp.png" width=150 /> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/3/35/Groningen2012_ADPigmentsBig.png" class="preview" width=400 /> | ||
+ | </a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | <td class="accPic"> | ||
+ | <ul class="hoverbox" style="margin-left: 30px"> | ||
+ | <li> | ||
+ | <a href="#"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/9/93/Groningen2012_ADConstruct_yellow.png" width=220 /> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/2/21/Groningen2012_ADConstructBig.png" class="preview" width=400 /> | ||
+ | </a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td class="accCap"> | ||
+ | <p class="small"> | ||
+ | <font color=#FF6700><br><br><b>5.</b></font> We made <a class="inlink" href="https://2012.igem.org/Team:Groningen/pigmentproduction">AmilCP and AmilGFP</a> suitable for expression in <i>Bacillus subtilis</i>. | ||
+ | <br> | ||
+ | These chromoproteins can be of significant value to the other <i>Bacillus subtilis</i> users in the BioBrick community. | ||
+ | <br><br><br> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td class="accCap"> | ||
+ | <p class="small"> | ||
+ | <font color=#FF6700><b>6.</b></font> Most importantly: we developed a <a class="inlink" href="https://2012.igem.org/Team:Groningen/Construct">construct</a> which makes <i>Bacillus subtilis</i> sense spoiled meat and produce an output in the form of a yellow or purple pigment visible by naked eye. | ||
+ | <br><br><br> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | </body> | ||
</html> | </html> | ||
{{Template:SponsorsGroningen2012}} | {{Template:SponsorsGroningen2012}} |
Latest revision as of 02:00, 27 October 2012
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Every year, one third of global food production -1.3 billion tons of food- is thrown away, partially due to the “best before” dating system.
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Tested a construct with downregulated promoter WapA
Performed enhanced construct characterization
Characterized the influence of oxygen on germination and growth within the sticker
Expanded the scope of the market survey
Collaborated with Cambridge 2012
Hover your mouse over the pictures to see more!
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2. We explored the definition of spoiled meat and ways to check meat spoilage. We identified various compounds present in spoiled meat. |
3. We identified spoiled meat sensors by transcriptome analysis. |
4. Backbone pSac-Cm: easy cloning in |
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6. Most importantly: we developed a construct which makes Bacillus subtilis sense spoiled meat and produce an output in the form of a yellow or purple pigment visible by naked eye.
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