Team:OUC-China/Project/DesignMaking/Abstract

From 2012.igem.org

(Difference between revisions)
(Created page with "<html> <head> <link rel="stylesheet" href="http://1.oucigem.sinaapp.com/oucigem2012-nav.css"><!--nav css--> <script type="text/javascript" src="http://ajax.googleapis.com/ajax/li...")
 
(14 intermediate revisions not shown)
Line 1: Line 1:
<html>
<html>
<head>
<head>
 +
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
<link rel="stylesheet" href="http://1.oucigem.sinaapp.com/oucigem2012-nav.css"><!--nav css-->
<link rel="stylesheet" href="http://1.oucigem.sinaapp.com/oucigem2012-nav.css"><!--nav css-->
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.7.1/jquery.min.js"></script><!--jquery js-->
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.7.1/jquery.min.js"></script><!--jquery js-->
<script type="text/javascript" src="http://1.oucigem.sinaapp.com/OUC-nav.js"></script><!--nav js-->
<script type="text/javascript" src="http://1.oucigem.sinaapp.com/OUC-nav.js"></script><!--nav js-->
 +
<link rel="stylesheet" href="http://1.oucigem.sinaapp.com/style.css"><!--slidenav css-->
 +
<style>
<style>
*{
*{
margin:0;
margin:0;
padding:0;
padding:0;
 +
}
 +
ul{
 +
list-style: none;
}
}
body{
body{
Line 13: Line 19:
padding:0;
padding:0;
margin:0;
margin:0;
-
overflow-x:hidden;
+
overflow-x:auto;
font: 500 12px/1.625 "Helvetica Neue", Helvetica, Arial, sans-serif;
font: 500 12px/1.625 "Helvetica Neue", Helvetica, Arial, sans-serif;
 +
}
 +
#top-section{
 +
width:99%;
}
}
#page{
#page{
-
margin-left:60px;
+
margin-left:0px;
 +
}
 +
#me2nu {
 +
position: absolute;
 +
top: 100PX;
 +
left: 200px;
 +
width: 95px;
 +
float: left;
 +
padding-top: 25px;
 +
margin-left: 10px;
 +
margin-right: -10px;
}
}
.article-list {
.article-list {
Line 51: Line 70:
}
}
.article-list .list-box p{
.article-list .list-box p{
-
font-family:Georgia;
+
font-family:Arial, sans-serif;
font-size:16px;
font-size:16px;
color: #222;
color: #222;
-
}
 
-
.article-list .list-text span{
 
-
margin-left:15px;
 
-
}
 
-
.article-list .list-text sub{
 
-
margin-left:90px;
 
-
}
 
-
.article-list .list-text link{
 
-
text-decoration:underline;
 
}
}
.article-list .start{
.article-list .start{
Line 129: Line 139:
#slidenav li.gas-vesicle a { background-position: -55px -150px; width: 152px; height: 32px; }
#slidenav li.gas-vesicle a { background-position: -55px -150px; width: 152px; height: 32px; }
#slidenav li.gas-vesicle a span { background-position: 0 -150px; }
#slidenav li.gas-vesicle a span { background-position: 0 -150px; }
-
+
#ouc-footer{
-
#ouc-footer{
+
text-align:center;
text-align:center;
margin-top:25px;
margin-top:25px;
-
margin-left:-100px;
+
margin-left:10px;
-
 
+
        width:800px;
}
}
#footer-box{
#footer-box{
height: 50px;
height: 50px;
-
width:100%;
+
width:1349px;
margin-top:0;
margin-top:0;
margin-bottom:-10px;
margin-bottom:-10px;
background-color:#1487DF;
background-color:#1487DF;
-
border-top-width:10px;
+
border:none;
-
border-top-color:#F7F5F5;
+
padding-left:0;
-
border-bottom:none;
+
padding-right:0;
}
}
#footer a
#footer a
Line 173: Line 182:
     color: #2d7ca3;
     color: #2d7ca3;
}
}
 +
.mini-riboon{
 +
position: relative;
 +
z-index:0;
 +
left: 1001px;
 +
top: 90px;
 +
width: 106px;
 +
height: 45px;
 +
}
</style>
</style>
</head>
</head>
Line 180: Line 197:
</div>
</div>
<ul class="menu">
<ul class="menu">
-
<li> <a href="#">Home</a> </li>
+
<li> <a href="https://2012.igem.org/Team:OUC-China">Index</a> </li>
-
<li> <a href="#">Project</a> </li>
+
<li> <a href="https://2012.igem.org/Team:OUC-China/Project/Overview">Project</a> </li>
-
<li> <a href="#">Modeing</a> </li>
+
<li> <a href="https://2012.igem.org/Team:OUC-China/Modeling/Overview">Modeling</a> </li>
-
<li> <a href="#">Human Practice</a> </li>
+
<li> <a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Overview">Human Practice</a> </li>
-
<li> <a href="#">Team</a> </li>
+
<li> <a href="https://2012.igem.org/Team:OUC-China/Team/members">Team</a> </li>
-
<li> <a href="#">Parts</a> </li>
+
<li> <a href="https://2012.igem.org/Team:OUC-China/Parts">Parts</a> </li>
-
<li> <a href="#">Safety</a> </li>
+
<li> <a href="https://2012.igem.org/Team:OUC-China/Safety">Safety</a> </li>
-
<li> <a href="#">Note</a> </li>
+
<li> <a href="https://2012.igem.org/Team:OUC-China/labnote">Note</a> </li>
</ul>
</ul>
</div>
</div>
Line 193: Line 210:
<ul class="submenu">
<ul class="submenu">
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/a/af/Projectoverview.jpg" />
-
+
Home
</a>
</a>
</li>
</li>
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China#hmo">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/4/49/Humanpracticeoverview.jpg" />
-
+
HumanPractice index
</a>
</a>
</li>
</li>
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China/Judgingform">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/6/6e/Juding-form.jpg" />
-
+
JudgingForm
</a>
</a>
</li>
</li>
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China/contactus">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/1/1e/Contact-us.jpg" />
 +
Contact Us
 +
</a>
 +
</li>
 +
</ul>
 +
<ul class="submenu">
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/Project/Overview">
 +
<img src="https://static.igem.org/mediawiki/2012/5/59/Ouc-nav-project-overview.jpg" />
 +
Project Overview
 +
</a>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/Project/Sensor/AbstractandBackground">
 +
<img src="https://static.igem.org/mediawiki/2012/1/15/Ouc-nav-project-secsor.jpg" />
 +
Sensor
 +
</a>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract">
 +
<img src="https://static.igem.org/mediawiki/2012/3/34/Ouc-nav-device.jpg" />
 +
Decision-making Device
 +
</a>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/Project/GVP/GasandBackground">
 +
<img src="https://static.igem.org/mediawiki/2012/5/57/Ouc-nav-project-gvp.jpg" />
 +
Gas vesicle
</a>
</a>
</li>
</li>
Line 219: Line 263:
<ul class="submenu">
<ul class="submenu">
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China/Modeling/ODEModel">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/f/f6/Ouc-nav-modeling-ode.jpg" />
-
+
ODEModel
</a>
</a>
</li>
</li>
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China/Modeling/ParameterSensitivityAnalysis">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/c/cf/Ouc-nav-modeling-sensertitive.jpg" />
-
+
Parameter Sensitivity Analysis
</a>
</a>
</li>
</li>
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China/Modeling/ParameterSweep">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/0/07/Ouc-nav-modeling-sweep.jpg" />
-
+
Parameter Sweep
</a>
</a>
</li>
</li>
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China/Modeling/NoiseAnalysis">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/0/04/Ouc-nav-modeling-noise.jpg" />
-
+
Noise Analysis
</a>
</a>
</li>
</li>
Line 245: Line 289:
<ul class="submenu">
<ul class="submenu">
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Overview">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/6/61/Ouc-nav-humanoverview.jpg" />
-
+
HumanPractice Overview
</a>
</a>
</li>
</li>
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Communication">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/c/cc/Ouc-nav-Communication.jpg" />
-
+
Meeting and Academic Communication
</a>
</a>
</li>
</li>
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/Camps">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/8/8d/Ouc-nav-humcamp.jpg" />
-
+
Camps, Class and Lectures
</a>
</a>
</li>
</li>
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China/HumanPractice/SpecialHM">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/b/b7/Ouc-nav-hmspcial.jpg" />
-
+
Special HP
</a>
</a>
</li>
</li>
Line 271: Line 315:
<ul class="submenu">
<ul class="submenu">
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China/Team/members">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/6/6d/Ouc-nav-team.jpg" />
-
+
Team Members
</a>
</a>
</li>
</li>
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China/Team/instructor">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/3/35/Ouc-nav-instructor.jpg" />
-
+
Instructors
</a>
</a>
</li>
</li>
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China/Team/AcknowledgementandCooperation">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/a/a6/Ouc-nav-co.jpg" />
-
+
Acknowledgement&Cooperation
</a>
</a>
</li>
</li>
<li>
<li>
-
<a href="">
+
<a href="https://2012.igem.org/Team:OUC-China/Team/Lab">
-
<img src="" />
+
<img src="https://static.igem.org/mediawiki/2012/a/a7/Ouc-nav-lab.jpg" />
-
+
Lab
 +
</a>
 +
</li>
 +
</ul>
 +
<ul class="submenu">
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/Parts">
 +
<img src="https://static.igem.org/mediawiki/2012/f/f9/Ouc-nav-parts.jpg" />
 +
Parts
 +
</a>
 +
</li>
 +
</ul>
 +
<ul class="submenu">
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/Safety">
 +
<img src="https://static.igem.org/mediawiki/2012/5/5d/Ouc-nav-safety.jpg" />
 +
Safety
 +
</a>
 +
</li>
 +
</ul>
 +
<ul class="submenu">
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/labnote">
 +
<img src="https://static.igem.org/mediawiki/2012/8/8b/Ouc-nav-labnote.jpg" />
 +
Labnote
 +
</a>
 +
</li>
 +
<li>
 +
<a href="https://skydrive.live.com/redir?resid=6A13E80E2A176A23!249&authkey=!AGsNOi9excznIt8">
 +
<img src="https://static.igem.org/mediawiki/2012/4/4e/Ouc-nav-modelingnote.jpg" />
 +
Modeling Note
 +
</a>
 +
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:OUC-China/protocol">
 +
<img src="https://static.igem.org/mediawiki/2012/7/7d/Ouc-pro.png" />
 +
Protocols
</a>
</a>
</li>
</li>
Line 299: Line 379:
     <div id="page">
     <div id="page">
                    
                    
-
<div id="slidenav">
+
  <div id="me2nu">
-
<div id="slidenav-bar">
+
<div id="menu_bar">
-
<ul id="social" class="group">
+
<ul>
-
+
<li  id="menu_home"><a href="https://2012.igem.org/Team:OUC-China" title="Back" class="home">HOME</a></li>
-
<li class="meet"><a href="#"><span>meet</span></a></li>
+
<li id="menu_news"><a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract" title="Abstract" class="news">Abstract</a></li>
-
<li class="sensor"><a href="#"><span></span>sensor</a></li>
+
            <li id="menu_home"><a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Background" title="Background" class="home">Background</a></li>
-
<li class="decision-making"><a href="#"><span></span>decision-making</a></li>
+
<li id="menu_design"><a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/GoalandDesign" title="Goal&Design" class="design">Goal&Design</a></li>
-
<li class="gas-vesicle"><a href="#"><span></span>gas vesicle</a></li>
+
<li  id="menu_others"><a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/Result" title="Result" class="others">Result</a></li>
-
+
<li id="menu_books"><a href="https://2012.igem.org/Team:OUC-China/Project/DesignMaking/DiscussionandFuture" title="Discussion" class="books">Discussion</a></li>
-
</ul>
+
</ul>
-
</div>
+
</div>
-
</div>
+
</div>  
 +
<div class="mini-riboon"><img src="https://static.igem.org/mediawiki/2012/0/03/Ouc-mini-ribbon-decision-making.png"></div>
<div class="article-list">
<div class="article-list">
<div class="start"></div>
<div class="start"></div>
                 <div class="list-box">
                 <div class="list-box">
<div class="list-text">
<div class="list-text">
-
<h1>Abstract</h1>
+
<h1 style="border:none;">Abstract</h1>
<p> We are using small RNA to build decision-making devices, Ratio Sensor and Comparator,which process two independent inputs into detectable output.
<p> We are using small RNA to build decision-making devices, Ratio Sensor and Comparator,which process two independent inputs into detectable output.
<br/>
<br/>
<br/>
<br/>
-
<h2>What our decision-making device wants to do?</h2>
+
<h2>What our decision-making device could do?</h2>
<p><span></span>Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase, to this end, we facilitate two well studied promoters(Plac and Ptet) and GFP to construct a quantitative test platform, where GFP mRNA concentration will be monitored indirectly through fluorospectro photometer.Of course,IPTG and aTc serve as two substitute inputs instead of nitrate and phosphate. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.
<p><span></span>Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase, to this end, we facilitate two well studied promoters(Plac and Ptet) and GFP to construct a quantitative test platform, where GFP mRNA concentration will be monitored indirectly through fluorospectro photometer.Of course,IPTG and aTc serve as two substitute inputs instead of nitrate and phosphate. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.
<br/>
<br/>
Line 363: Line 444:
</body>
</body>
<script src="http://www.google-analytics.com/urchin.js" type="text/javascript">
<script src="http://www.google-analytics.com/urchin.js" type="text/javascript">
 +
</script>
 +
<script>
 +
var selected_ele = $('#me2nu ul li.selected');
 +
$('#me2nu ul li').hover(function(){
 +
selected_ele.removeClass('selected');
 +
$(this).addClass('selected');
 +
},function() {
 +
$(this).removeClass('selected');
 +
selected_ele.addClass('selected');
 +
});
</script>
</script>
<script type="text/javascript">
<script type="text/javascript">

Latest revision as of 09:08, 26 October 2012

Abstract

We are using small RNA to build decision-making devices, Ratio Sensor and Comparator,which process two independent inputs into detectable output.

What our decision-making device could do?

Our decision-making device aims at processing the concentration change of nitrate or phosphate into visible buoyancy increase, to this end, we facilitate two well studied promoters(Plac and Ptet) and GFP to construct a quantitative test platform, where GFP mRNA concentration will be monitored indirectly through fluorospectro photometer.Of course,IPTG and aTc serve as two substitute inputs instead of nitrate and phosphate. Comparator recognizes the difference of two inputs quantitatively ,while ratio sensor could senses a particular ratio between two inputs.

How?

Both comparator and ratio sensor facilitate a 3-ternary system composed of two small RNA and GFP mRNA.They are generated from plasmid and then diffuse in cytosol ;considering RNA pairing reaction as chemical reaction,we will see the probability of collision is determined by their concentration,and the probability of pairing after collision is determined by their affinity(that is reaction constant k). The concentration change of aTc and IPTG in media leads to the transcription rate change of small RNA(αs)in this 3-ternary system, we hope the interactions between three RNA will lead to interesting behaviours of our devices like sensing the difference between two inputs quantitatively(Comparator) or the ratio of two inputs(Ratio senor).



Retrieved from "http://2012.igem.org/Team:OUC-China/Project/DesignMaking/Abstract"