Team:UNAM Genomics Mexico/prueba
From 2012.igem.org
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4.CI 02 E/P. <br /> | 4.CI 02 E/P. <br /> | ||
5.P4 PCR. <br /> | 5.P4 PCR. <br /> | ||
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4.CI 02 E/P. <br /> | 4.CI 02 E/P. <br /> | ||
5.P4 PCR. <br /> | 5.P4 PCR. <br /> | ||
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6.LasR 00179 new primers. <br /> | 6.LasR 00179 new primers. <br /> | ||
7.LasR 00179 new primers (-).<br /> | 7.LasR 00179 new primers (-).<br /> | ||
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<img src='https://static.igem.org/mediawiki/2012/5/55/UnamgenomicsoANDcadmio09_14_2012_5.jpg' height="300"/> | <img src='https://static.igem.org/mediawiki/2012/5/55/UnamgenomicsoANDcadmio09_14_2012_5.jpg' height="300"/> | ||
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<p class='captionInside'>1.ArsR-CzrA 97 E/P. <br /> | <p class='captionInside'>1.ArsR-CzrA 97 E/P. <br /> | ||
2.ArsR-CzRA 98 E/P. <br /> | 2.ArsR-CzRA 98 E/P. <br /> | ||
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+ | <div class='thumbnailWrapper'> | ||
+ | <ul> | ||
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+ | <img src='https://static.igem.org/mediawiki/2012/2/2f/UnamgenomicsoANDcadmio09_14_2012_6.jpg' height="300"/> | ||
+ | <div class='captionaqua'> | ||
+ | <p class='captionInside'>1.ArsR-CzrA 97 E/P. <br /> | ||
+ | 2.ArsR-CzRA 98 E/P. <br /> | ||
+ | 3.1 kb ladder. <br /> | ||
+ | 4.ArsR-CzRA 99 E/P. <br /> | ||
+ | 5.CI 02 E/P. <br /> | ||
+ | 6.AmyE 5’ 97 E/S. <br /> | ||
+ | 7.AmyE 5’ 98 E/S. <br /> | ||
+ | 8.1 kb ladder. <br /> | ||
+ | 9.AmyE 5’ 99 E/S. <br /> | ||
+ | 10.LasR PCR. <br /> | ||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <br /><br /> | ||
+ | </html> | ||
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+ | <h2>09/14/12</h2><br /> | ||
+ | We extracted the correct band by kit [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Gel_extraction_protocol | GEL EXTRACTION PROTOCOL]]. <br /><br /> | ||
+ | >We transformed ligation psB1c3+ArsR-CzrA 97/98/99/CI in competent DH5α cells from the pBad/pXyl AND team and in our own DH5α competent cells[[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Transformation | TRANSFORMATION PROTOCOL]]. <br /><br /> | ||
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+ | <h2>09/15/12</h2><br /> | ||
+ | >We checked our transformations an did liquid cultures and passed the colonies to new plates [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Liquid_culture | LIQUID CULTURE PROTOCOL]]. <br /> | ||
+ | >We digested E0040+B0014 [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Digestion_protocol_.2820_.C2.B5l.29 | DIGESTIONS PROTOCOL]]. <br /> | ||
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+ | >We ligated 97/98/99 psBIC3 [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Ligation | LIGATION PROTOCOL]]. <br /> | ||
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+ | <html> | ||
+ | <div class='thumbnailWrapper'> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <img src='https://static.igem.org/mediawiki/2012/a/a8/UnamgenomicsoANDcadmio09_16_2012.jpg' height="300"/> | ||
+ | <div class='captionaqua'> | ||
+ | <p class='captionInside'>1.AmyE 5’ 97 S/P. <br /> | ||
+ | 2. | ||
+ | 3.AmyE 5’ 98 S/P. <br /> | ||
+ | 4. | ||
+ | 5.1 kb ladder. <br /> | ||
+ | 6.AmyE 5’ 99 S/P. <br /> | ||
+ | 7.AmyE 5’ 99 S/P. <br /> | ||
+ | 8.E0040 B0014 X/P. <br /> | ||
+ | 9. | ||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
<br /><br /> | <br /><br /> | ||
+ | </html> | ||
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+ | <html> | ||
+ | <div class='thumbnailWrapper'> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <img src='https://static.igem.org/mediawiki/2012/8/89/UnamgenomicsoANDcadmio09_16_2012_2.jpg' width="300"/> | ||
+ | <div class='captiongray'> | ||
+ | <p class='captionInside'>1.E0040 B0014 X/P to extract. <br /> | ||
+ | 2.1 kb ladder. <br /> | ||
+ | 3.Lysis. <br /> | ||
+ | 4.-19. Amy 5’ 99/97/P4CIE1010B0014 lysis. <br /> | ||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
<br /><br /> | <br /><br /> | ||
+ | </html> | ||
+ | <html> | ||
+ | <div class='thumbnailWrapper'> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <img src='https://static.igem.org/mediawiki/2012/b/b0/UnamgenomicsoANDcadmio09_16_2012_3.jpg' height="300"/> | ||
+ | <div class='captionnaranja'> | ||
+ | <p class='captionInside'>1.LasR PCR. <br /> | ||
+ | 2.LasR PCR. <br /> | ||
+ | 3.LasR PCR. <br /> | ||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <br /><br /> | ||
+ | </html> | ||
+ | <html> | ||
+ | <div class='thumbnailWrapper'> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <img src='https://static.igem.org/mediawiki/2012/c/c1/UnamgenomicsoANDcadmio09_16_2012_4.jpg' height="300"/> | ||
+ | <div class='captionrojo'> | ||
+ | <p class='captionInside'>1.1 kb ladder. <br /> | ||
+ | 2.Purified P4. <br /> | ||
+ | 3.Purified LasR. <br /> | ||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <br /><br /> | ||
+ | </html> | ||
+ | <html> | ||
+ | <div class='thumbnailWrapper'> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <img src='https://static.igem.org/mediawiki/2012/3/38/UnamgenomicsoANDcadmio09_16_2012_5.jpg' height="300"/> | ||
+ | <div class='captionverde'> | ||
+ | <p class='captionInside'>Top part is degraded. | ||
+ | Bottom: | ||
+ | 1.99 E/P. <br /> | ||
+ | 2.99 E/P. <br /> | ||
+ | 3.CI 02 E/P. <br /> | ||
+ | 4.CI 02 E/P. <br /> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <br /><br /> | ||
+ | </html> | ||
+ | <h2>09/16/12</h2><br /> | ||
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+ | <html> | ||
+ | <div class='thumbnailWrapper'> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <img src='https://static.igem.org/mediawiki/2012/d/d6/UnamgenomicsoANDcadmio09_18_2012.jpg' height="300"/> | ||
+ | <div class='captionverde'> | ||
+ | <p class='captionInside'>A3/Pveg lysis. <br /><br /> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <br /><br /> | ||
+ | </html> | ||
+ | |||
+ | <html> | ||
+ | <div class='thumbnailWrapper'> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <img src='https://static.igem.org/mediawiki/2012/8/8f/UnamgenomicsoANDcadmio09_18_2012_2.jpg' height="300"/> | ||
+ | <div class='captionverde'> | ||
+ | <p class='captionInside'>1.-12. psCIC3 omega lysis. <br /> | ||
+ | 13. 1 kb ladder. <br /> | ||
+ | 14. psBIc3-GusA. <br /> | ||
+ | <br /> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <br /><br /> | ||
+ | </html> | ||
+ | |||
+ | <html> | ||
+ | <div class='thumbnailWrapper'> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <img src='https://static.igem.org/mediawiki/2012/e/ec/UnamgenomicsoANDcadmio09_18_2012_3.jpg' height="300"/> | ||
+ | <div class='captionverde'> | ||
+ | <p class='captionInside'> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <br /><br /> | ||
+ | </html> | ||
+ | |||
+ | <html> | ||
+ | <div class='thumbnailWrapper'> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <img src='https://static.igem.org/mediawiki/2012/f/fa/UnamgenomicsoANDcadmio09_18_2012_4.jpg' height="300"/> | ||
+ | <div class='captionverde'> | ||
+ | <p class='captionInside'>Digested A3 and pVeg with E/P. <br /> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <br /><br /> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | <html> | ||
+ | <div class='thumbnailWrapper'> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <img src='https://static.igem.org/mediawiki/2012/9/94/UnamgenomicsoANDcadmio09_18_2012_5.jpg' height="300"/> | ||
+ | <div class='captionverde'> | ||
+ | <p class='captionInside'>1.ArsR-CzrA 97 E/P. <br /> | ||
+ | 2.ArsR-CzrA 98 E/P. <br /> | ||
+ | 3.ArsR-CzrA 99 E/P. <br /> | ||
+ | 4.02-CI E/P. <br /> | ||
+ | 5.PCR P4 purified. <br /> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <br /><br /> | ||
+ | </html> | ||
+ | |||
+ | <h2>09/18/12</h2><br /> | ||
}} | }} |
Latest revision as of 06:47, 26 October 2012
= MAY =
Contents[hide] |
09/14/12
We extracted the correct band by kit GEL EXTRACTION PROTOCOL.
>We transformed ligation psB1c3+ArsR-CzrA 97/98/99/CI in competent DH5α cells from the pBad/pXyl AND team and in our own DH5α competent cells TRANSFORMATION PROTOCOL.
09/15/12
>We checked our transformations an did liquid cultures and passed the colonies to new plates LIQUID CULTURE PROTOCOL.
>We digested E0040+B0014 DIGESTIONS PROTOCOL.
>We ligated 97/98/99 psBIC3 LIGATION PROTOCOL.
09/16/12