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Team:UNAM Genomics Mexico/Results/Bacillus
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| + | We know that everybody has wetlab problems, just like us. And we actually spend most of the summer looking to standardize the Bacillus subtilis transformation protocol. And we did it! So we are presenting you our new standard: | ||
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| + | [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Escherichia_coli_MC1061_competent_cells_protocol | '''Escherichia coli MC1061 competent cells protocol''']] Please click this link to see the complete protocol in our notebook protocols | ||
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| - | [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Escherichia_coli_MC1061_heat_shock_transformation_protocol Two-step Bacillus subtilis transformation procedure]] | + | [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Escherichia_coli_MC1061_heat_shock_transformation_protocol | '''Two-step Bacillus subtilis transformation procedure''']]Please click this link to see the complete protocol in our notebook protocols |
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Revision as of 03:05, 20 October 2012
Bacillus subtilis Results
We know that everybody has wetlab problems, just like us. And we actually spend most of the summer looking to standardize the Bacillus subtilis transformation protocol. And we did it! So we are presenting you our new standard:
Escherichia coli MC1061 competent cells protocol Please click this link to see the complete protocol in our notebook protocols
Two-step Bacillus subtilis transformation procedurePlease click this link to see the complete protocol in our notebook protocols
We notice that you not only need a plasmid to transform into B. subtilis, but a plasmid that has form multimers in a RecA+ E. coli and should be flanked by AmyE 5' & 3', so that when the plasmid is transformed into Bacillus, it does not get degradated, and it makes an integration into the genome.
