Team:TMU-Tokyo/Parts

From 2012.igem.org

(Difference between revisions)

Latest revision as of 02:30, 27 September 2012

Parts

<groupparts>iGEM012 TMU-Tokyo</groupparts>

■Favorite parts introduction

・BBa_K749025

pfrm-frmR-E0040-B0015-J23100-B0034-fdh-B0015

・BBa_K749015

J23100-B0034-fdh4A-fdh4B-B0015

・BBa_K749021

pfrm-frmR-E0040-B0015

Other parts are described in Parts Registry.

Formaldehyde monitoring and dehydrogenate device. The upstream is sensor for formaldehyde using frmR from Escherichia coli (Device1) and the downstream consecutively produces PFDH, formaldehyde dehydrogenase from Pseudomonas putida (Device2).
BBa_K749025, by itself, enables to observe the degree of formaldehyde decomposition with the naked eye.

Formate dehydrogenase device. BBa_K749015 produces formate dehydrogenase 4 from Methylobacterium extorquens consecutively (Device3).
Complete formaldehyde detoxification is realized by only BBa_K749025 and BBa_K749015.

Upstream of BBa_K749025, formaldehyde sensor. we use BBa_K749021 in assay for frmR inhibitory.

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-<b>■BBa_K749025</b><Br></p>
+<b>・BBa_K749025</b><Br></p>
 <IMG Src="https://static.igem.org/mediawiki/2012/e/ed/BBa_K749000.png"><Br>
 <p class="description3">pfrm-frmR-E0040-B0015-J23100-B0034-fdh-B0015</p><Br>
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+<Br>
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-<b>■BBa_K749015</b><Br></p>
+<b>・BBa_K749015</b><Br></p>
 <IMG Src="https://static.igem.org/mediawiki/2012/d/d5/BBa_K749001.png"><Br>
 <p class="description3">J23100-B0034-fdh4A-fdh4B-B0015</p><Br>
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 <p class="description">
-<b>■BBa_K749021</b><Br></p>
+<b>・BBa_K749021</b><Br></p>
 <IMG Src="https://static.igem.org/mediawiki/2012/9/94/BBa_K749002.png"><Br>
 <p class="description3">pfrm-frmR-E0040-B0015</p><Br>
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+Other parts are described in Parts Registry.<Br>
+<Br>
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 <p class="description">
 <Br><Br>
-Formaldehyde monitoring and dehydrogenate device. The upstream is sensor for formaldehyde (<A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_device1">Device1</A>) and the downstream consecutively produces PFDH, formaldehyde dehydrogenase from <i>Pseudomonas putida</i> (<A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_device2">Device2</A>).<Br>
+Formaldehyde monitoring and dehydrogenate device. The upstream is sensor for formaldehyde using frmR from <i>Escherichia coli</i> (<A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_device1">Device1</A>) and the downstream consecutively produces PFDH, formaldehyde dehydrogenase from <i>Pseudomonas putida</i> (<A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_device2">Device2</A>).<Br>
-BBa_K749000, by itself, enables to observe the degree of formaldehyde decomposition with the naked eye.<Br>
+BBa_K749025, by itself, enables to observe the degree of formaldehyde decomposition with the naked eye.<Br>
 <Br>
 <Br>
-Formate dehydrogenase device. BBa_K749001 produces formate dehydrogenase 4 from <i>Methylobacterium extorquens</i> consecutively (<A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_device3">Device3</A>).<Br>
+Formate dehydrogenase device. BBa_K749015 produces formate dehydrogenase 4 from <i>Methylobacterium extorquens</i> consecutively (<A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_device3">Device3</A>).<Br>
-Complete formaldehyde detoxification is realized by only BBa_K749000 and BBa_K749001.<Br>
+Complete formaldehyde detoxification is realized by only BBa_K749025 and BBa_K749015.<Br>
 <Br>
-Upstream of BBa_K749000, formaldehyde sensor. we use BBa_K749000 in assay for <i>frmR</i> inhibitory.
+Upstream of BBa_K749025, formaldehyde sensor. we use BBa_K749021 in assay for <i>frmR</i> inhibitory.