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Team:TMU-Tokyo/Notebook/Assay 1 Protocol and Result
From 2012.igem.org
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<Br> | <Br> | ||
<B>■Assay</B><Br> | <B>■Assay</B><Br> | ||
| - | Device1 Assay<Br> | + | <A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_0_Protocol_and_Result">Assay0</A><Br> |
| - | Device2 Assay<Br> | + | Device1 Assay</A><Br> |
| - | Device3 Assay<Br> | + | <A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_2_Protocol_and_Result">Device2 Assay<Br> |
| + | <A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Assay_3_Protocol_and_Result">Device3 Assay</A><Br> | ||
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<p class="description"> | <p class="description"> | ||
<b>■Assay1 Protocol</b><Br> | <b>■Assay1 Protocol</b><Br> | ||
| - | + | <Br> | |
| + | 1. In LB medium (liquid), wound bacteria frm-GFP, were incubated for 6 hours at room shaking culture.<Br> | ||
| + | 2. Was placed 5μl (0.04%) formalin solution to take the sample tube 500μl culture was diluted 200-fold there.<Br> | ||
| + | 3.4 Degrees, I was centrifuged for 2 minutes at 120rpm<Br> | ||
| + | 4. Supernatant was removed, I was vortexed<Br> | ||
| + | 5. Put on the preparations taking 3μl from the tube, I observed a cover glass.<Br> | ||
| + | 6. I observed as a control those that do not contain formaldehyde.<Br> | ||
| + | <Br> | ||
| + | |||
| + | |||
| + | </p> | ||
<p class="description"> | <p class="description"> | ||
<b>■Assay1 Result</b><Br> | <b>■Assay1 Result</b><Br> | ||
| + | GFP expression was not observed either. | ||
| + | 50 times, 100 times, 400 times, 600 times, 800 times, created in 1000-fold diluted solution of the same procedure, but I was the same experiment, the expression of GFP was not observed either. | ||
</p> | </p> | ||
Latest revision as of 02:26, 27 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
■Assay
Assay0
Device1 Assay
Device2 Assay
Device3 Assay
Assay 1
■Assay1 Protocol
1. In LB medium (liquid), wound bacteria frm-GFP, were incubated for 6 hours at room shaking culture.
2. Was placed 5μl (0.04%) formalin solution to take the sample tube 500μl culture was diluted 200-fold there.
3.4 Degrees, I was centrifuged for 2 minutes at 120rpm
4. Supernatant was removed, I was vortexed
5. Put on the preparations taking 3μl from the tube, I observed a cover glass.
6. I observed as a control those that do not contain formaldehyde.
■Assay1 Result
GFP expression was not observed either.
50 times, 100 times, 400 times, 600 times, 800 times, created in 1000-fold diluted solution of the same procedure, but I was the same experiment, the expression of GFP was not observed either.
