Team:Groningen/Project
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- | + | body { | |
- | + | font-family: Helvetica, Arial, sans-serif; | |
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- | + | } | |
- | + | z1 { | |
- | + | font-size:18pt; | |
- | + | line-height:21pt; | |
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color:white; | color:white; | ||
- | } | + | } |
+ | .ctd { | ||
+ | width: 140px; | ||
+ | height: 20px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | padding: 10px ; | ||
+ | margin: 30px auto; | ||
+ | color: #FFF; | ||
+ | } | ||
+ | .cte { | ||
+ | width: 170px; | ||
+ | height: 40px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | padding-right:10px; | ||
+ | padding-left:10px; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | margin: -30px auto; | ||
+ | color: #FFF; | ||
+ | } | ||
+ | </style> | ||
+ | <br><br> | ||
+ | <div class="cte"> | ||
+ | <div class="ctd"> | ||
+ | <z1 >Abstract</z1><br> | ||
+ | </div> | ||
+ | </div> | ||
+ | </body> | ||
+ | </head> | ||
+ | </html> | ||
+ | <html> | ||
+ | <body> | ||
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- | + | <head> | |
- | + | <style> | |
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- | + | ||
- | + | body { | |
- | { | + | font-family: Helvetica, Arial, sans-serif; |
- | + | ||
- | + | ||
- | . | + | } |
- | + | stickerP { | |
- | + | font-size:12pt; | |
- | } | + | line-height:12pt; |
+ | color:white; | ||
+ | } | ||
+ | .sticker1 { | ||
+ | width: 100px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | padding: 10px ; | ||
+ | margin: 0px auto; | ||
+ | color: #FFF; | ||
+ | } | ||
+ | .sticker2 { | ||
+ | left: 350px; | ||
+ | top:440px; | ||
+ | position:absolute; | ||
+ | width: 120px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | padding-right:10px; | ||
+ | padding-left:10px; | ||
+ | margin: 0 auto; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | color: #FFF; | ||
- | + | } | |
- | + | </style> | |
- | + | ||
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- | + | ||
- | + | ||
- | + | ||
- | + | <div class="sticker2"> | |
- | + | <div class="sticker1"> | |
- | + | <stickerP ><A HREF="https://2012.igem.org/Team:Groningen/Sticker" TARGET="_blank"><FONT COLOR=#ffffff>sticker</FONT></A></stickerP> | |
- | + | </div> | |
- | + | </div> | |
- | + | </body> | |
- | + | </head> | |
- | + | </html> | |
+ | <html> | ||
+ | <head> | ||
+ | <style> | ||
- | + | body { | |
- | { | + | font-family: Helvetica, Arial, sans-serif; |
- | + | ||
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- | + | ||
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- | + | } | |
- | { | + | GerminationP { |
- | + | font-size:12pt; | |
- | + | line-height:12pt; | |
- | } | + | color:white; |
+ | } | ||
+ | .Germination1 { | ||
+ | width: 100px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | padding: 10px ; | ||
+ | margin: 0px auto; | ||
+ | color: #FFF; | ||
+ | } | ||
+ | .Germination2 { | ||
+ | left: 530px; | ||
+ | top:440px; | ||
+ | position:absolute; | ||
+ | width: 120px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | padding-right:10px; | ||
+ | padding-left:10px; | ||
+ | margin: 0 auto; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | color: #FFF; | ||
+ | } | ||
</style> | </style> | ||
+ | |||
+ | <div class="Germination2"> | ||
+ | <div class="Germination1"> | ||
+ | <GerminationP ><A HREF="https://2012.igem.org/Team:Groningen/Germination" TARGET="_blank"><FONT COLOR=#ffffff>germination</FONT></A></GerminationP> | ||
+ | </div> | ||
+ | </div> | ||
+ | </body> | ||
</head> | </head> | ||
+ | </html> | ||
+ | <html> | ||
+ | <head> | ||
+ | <style> | ||
- | + | body { | |
- | + | font-family: Helvetica, Arial, sans-serif; | |
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- | + | ||
- | + | } | |
- | + | PBADmeat1P { | |
- | + | font-size:12pt; | |
- | + | line-height:12pt; | |
- | + | color:white; | |
- | + | } | |
- | + | .PBADmeat11 { | |
- | + | width: 100px; | |
- | + | text-align: center; | |
- | + | background: #000; | |
- | + | background-color: transparent; | |
- | + | background-color: rgba(0,0,0,0.3); | |
- | + | padding: 10px ; | |
- | + | margin: 0px auto; | |
- | + | color: #FFF; | |
- | + | } | |
- | + | .PBADmeat12 { | |
- | + | left: 350px; | |
- | + | top:480px; | |
- | + | position:absolute; | |
- | + | width: 120px; | |
- | + | text-align: center; | |
- | + | background: #000; | |
- | + | padding-right:10px; | |
- | + | padding-left:10px; | |
- | + | margin: 0 auto; | |
- | + | background-color: transparent; | |
- | + | background-color: rgba(0,0,0,0.3); | |
- | + | color: #FFF; | |
- | + | ||
- | + | } | |
- | + | </style> | |
- | + | ||
- | + | <div class="PBADmeat12"> | |
- | + | <div class="PBADmeat11"> | |
- | + | <PBADmeat1P ><A HREF="https://2012.igem.org/Team:Groningen/identifyingbadvolatiles" TARGET="_blank"><FONT COLOR=#ffffff>volatiles</FONT></A></PBADmeat1> | |
- | + | ||
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- | + | ||
</div> | </div> | ||
</div> | </div> | ||
+ | </body> | ||
+ | </head> | ||
+ | </html> | ||
+ | <html> | ||
+ | <head> | ||
+ | <style> | ||
- | + | body { | |
+ | font-family: Helvetica, Arial, sans-serif; | ||
- | + | } | |
- | + | identificationP { | |
- | + | font-size:12pt; | |
- | + | line-height:12pt; | |
- | + | color:white; | |
- | + | } | |
- | + | .identification1 { | |
- | + | width: 100px; | |
+ | text-align: center; | ||
+ | background: #000; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | padding: 10px ; | ||
+ | margin: 0px auto; | ||
+ | color: #FFF; | ||
+ | } | ||
+ | .identification2 { | ||
+ | left: 530px; | ||
+ | top:480px; | ||
+ | position:absolute; | ||
+ | width: 120px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | padding-right:10px; | ||
+ | padding-left:10px; | ||
+ | margin: 0 auto; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | color: #FFF; | ||
- | + | } | |
- | + | </style> | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | </ | + | |
- | < | + | <div class="identification2"> |
- | < | + | <div class="identification1"> |
- | < | + | <identificationP ><A HREF="https://2012.igem.org/Team:Groningen/identifyingPBADmeat" TARGET="_blank"><FONT COLOR=#ffffff>identification</FONT></A></identificationP> |
- | </ | + | </div> |
+ | </div> | ||
+ | </body> | ||
+ | </head> | ||
+ | </html> | ||
+ | <html> | ||
+ | <head> | ||
+ | <style> | ||
- | + | body { | |
- | + | font-family: Helvetica, Arial, sans-serif; | |
- | + | ||
- | + | ||
- | + | } | |
- | + | PigmentsP { | |
- | + | font-size:12pt; | |
- | + | line-height:12pt; | |
- | + | color:white; | |
- | + | } | |
- | + | .Pigments1 { | |
- | + | width: 100px; | |
+ | text-align: center; | ||
+ | background: #000; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | padding: 10px ; | ||
+ | margin: 0px auto; | ||
+ | color: #FFF; | ||
+ | } | ||
+ | .Pigments2 { | ||
+ | left: 350px; | ||
+ | top:520px; | ||
+ | position:absolute; | ||
+ | width: 120px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | padding-right:10px; | ||
+ | padding-left:10px; | ||
+ | margin: 0 auto; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | color: #FFF; | ||
- | + | } | |
- | + | </style> | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | </ | + | |
- | < | + | <div class="Pigments2"> |
- | < | + | <div class="Pigments1"> |
- | < | + | <PigmentsP ><A HREF="https://2012.igem.org/Team:Groningen/Pigments" TARGET="_blank"><FONT COLOR=#ffffff>pigment</FONT></A></Pigments> |
- | </ | + | </div> |
+ | </div> | ||
+ | </body> | ||
+ | </head> | ||
+ | </html> | ||
+ | <html> | ||
+ | <head> | ||
+ | <style> | ||
- | + | body { | |
- | + | font-family: Helvetica, Arial, sans-serif; | |
- | + | ||
- | + | ||
- | + | } | |
- | + | ConstructP { | |
- | + | font-size:12pt; | |
- | + | line-height:12pt; | |
- | + | color:white; | |
- | + | } | |
- | + | .Construct1 { | |
- | + | width: 100px; | |
+ | text-align: center; | ||
+ | background: #000; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | padding: 10px ; | ||
+ | margin: 0px auto; | ||
+ | color: #FFF; | ||
+ | } | ||
+ | .Construct2 { | ||
+ | left: 530px; | ||
+ | top:520px; | ||
+ | position:absolute; | ||
+ | width: 120px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | padding-right:10px; | ||
+ | padding-left:10px; | ||
+ | margin: 0 auto; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | color: #FFF; | ||
- | + | } | |
- | + | </style> | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | </ | + | |
- | < | + | <div class="Construct2"> |
- | < | + | <div class="Construct1"> |
- | < | + | <ConstructP ><A HREF="https://2012.igem.org/Team:Groningen/Construct" TARGET="_blank"><FONT COLOR=#ffffff>construct</FONT></A></ConstructP> |
- | < | + | </div> |
+ | </div> | ||
+ | </body> | ||
+ | </head> | ||
+ | </html> | ||
+ | <html> | ||
+ | <head> | ||
+ | <style> | ||
- | + | body { | |
- | + | font-family: Helvetica, Arial, sans-serif; | |
- | + | ||
- | + | ||
- | + | ||
+ | } | ||
+ | DeathP { | ||
+ | font-size:12pt; | ||
+ | line-height:12pt; | ||
+ | color:white; | ||
+ | } | ||
+ | .Death1 { | ||
+ | width: 100px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | padding: 10px ; | ||
+ | margin: 0px auto; | ||
+ | color: #FFF; | ||
+ | } | ||
+ | .Death2 { | ||
+ | left: 440px; | ||
+ | top:560px; | ||
+ | position:absolute; | ||
+ | width: 120px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | padding-right:10px; | ||
+ | padding-left:10px; | ||
+ | margin: 0 auto; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | color: #FFF; | ||
+ | |||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <div class="Death2"> | ||
+ | <div class="Death1"> | ||
+ | <DeathP ><A HREF="https://2012.igem.org/Team:Groningen/Death" TARGET="_blank"><FONT COLOR=#ffffff>kill switch</FONT></A></DeathP> | ||
+ | </div> | ||
+ | </div> | ||
+ | </body> | ||
+ | </head> | ||
+ | </html> | ||
+ | <html> | ||
+ | <div style="position:absolute; left: 80px; top: 500px;opacity:0.4; filter:alpha(opacity=40);z-index:-1;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150" height="150"> | ||
+ | </div> | ||
+ | |||
+ | <div style="position:absolute; left: 100px; top: 510px;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/c/c2/Groningen2012_RR_20120909_rotting.png" width="100" height="100"> | ||
+ | </div> | ||
+ | |||
+ | <div style="position:absolute; right: 80px; top: 500px;opacity:0.4; filter:alpha(opacity=40); z-index:-1;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150" height="150"> | ||
+ | </div> | ||
+ | |||
+ | <div style="position:absolute; right: 110px; top: 525px;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/e/e7/Groningen2012_RR20120909_capsule.png" width="100" height="100"> | ||
+ | </div> | ||
+ | |||
+ | <div style="position:absolute; left: 0px; top: 300px;opacity:0.4; filter:alpha(opacity=40); z-index:-1;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150" height="150"> | ||
+ | </div> | ||
+ | |||
+ | <div style="position:absolute; right: 20px; top: 320px;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/2/29/Groningen2012_RR20120909_badmeat.png" width="100" height="100"> | ||
+ | </div> | ||
+ | |||
+ | <div style="position:absolute; right: 0px; top: 300px;opacity:0.4; filter:alpha(opacity=40); z-index:-1;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150" height="150"> | ||
+ | </div> | ||
+ | |||
+ | <div style="position:absolute; left: 20px; top: 330px;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/1/15/Groningen2012_RR20120909_construct.png" width="100" height="100"> | ||
+ | </div> | ||
+ | </body> | ||
+ | |||
+ | </html> | ||
+ | <html> | ||
+ | <head> | ||
+ | <style type="text/css"> | ||
+ | |||
+ | p.marginleft | ||
+ | { | ||
+ | margin-top: 0px; | ||
+ | margin-left:150px; | ||
+ | font-size:13pt; | ||
+ | line-height:15pt; | ||
+ | margin-right:150px; | ||
+ | color:white; | ||
+ | } | ||
+ | p.orange | ||
+ | { | ||
+ | margin-top:0px; | ||
+ | margin-left:150px; | ||
+ | font-size:14pt; | ||
+ | line-height:16pt; | ||
+ | margin-right:150px; | ||
+ | color: #FF6700; | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | p.marginspace | ||
+ | { | ||
+ | font-size:13pt; | ||
+ | line-height:15pt; | ||
+ | color:white; | ||
+ | |||
+ | margin-top:0px; | ||
+ | margin-bottom:200px; | ||
+ | margin-right:0px; | ||
+ | margin-left:300px; | ||
+ | margin-right:300px; | ||
+ | } | ||
+ | p.centered | ||
+ | { | ||
+ | font-size:13pt; | ||
+ | line-height:15pt; | ||
+ | color:white; | ||
+ | |||
+ | margin-top:0px; | ||
+ | margin-bottom:0px; | ||
+ | margin-right:300px; | ||
+ | margin-left:300px; | ||
+ | } | ||
+ | |||
+ | z2 { | ||
+ | font-size:18pt; | ||
+ | line-height:21pt; | ||
+ | color:white; | ||
+ | } | ||
+ | .ctd2 { | ||
+ | width: 300px; | ||
+ | height: 20px; | ||
+ | text-align: center; | ||
+ | background: #000; | ||
+ | background-color: transparent; | ||
+ | background-color: rgba(0,0,0,0.3); | ||
+ | padding: 10px ; | ||
+ | margin: 30px auto; | ||
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<br><br><br> | <br><br><br> | ||
+ | Every year, one third of global food production -1.3 billion tons of food- is thrown away, partially due to the “best before” dating system. <font color=#FF6700><b>iGEM Groningen 2012</b></font> seeks to provide an alternative method of assessing edibility: the <font color=#FF6700><b>Food Warden</b></font>. It uses an <font color=#FF6700><b>engineered strain </b></font> of <i>Bacillus subtilis</i> to detect and report volatiles in spoiling meat. The introduced <font color=#FF6700><b>genetic construct</b></font> uses a promoter to trigger a pigment coding gene. This promoter, <font color=#FF6700><b>identified by microarray analysis</b></font>, is significantly up-regulated in the presence of <font color=#FF6700><b>volatiles from spoiling meat</b></font>. The activity of the <font color=#FF6700><b>promoter</b></font> regulates the expression of the <font color=#FF6700><b>pigment reporter</b></font> and will be visible to the naked eye. For safe usage of the system, spores of our engineered strain are placed into one half of a semi-permeable <font color=#FF6700><b>capsule</b></font>, the second containing a calibrated amount of nutrients. Breaking the barrier between the two compartments allows <font color=#FF6700><b>germination and growth</b></font>, thereby activating the <font color=#FF6700><b>spoiling meat sensor</b></font>.<br><br> | ||
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+ | <br><br><br><br><br><br><br><br><br><br><br> | ||
+ | <div class="cte2"> | ||
+ | <div class="ctd2"> | ||
+ | <a name="MainAcc"></a><z2 >Our main accomplishments</z2><br> | ||
+ | </div> | ||
+ | </div> | ||
+ | <br><br> | ||
+ | <p class=orange>In the lab</p> | ||
+ | <p class="marginleft"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Most importantly: we developed a construct which makes <i>Bacillus subtilis</i> sense spoiled meat and produce an output in the form of a yellow pigment visible by naked eye.<br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Design of the “<a href="https://2012.igem.org/Team:Groningen/Sticker">Sticker</a>”: semi-permeable capsule: bacteria are kept inside, volatiles can go through. Proof that <i>Bacillus subtilis</i> grows inside the sticker.<br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Development of BioBrick psac-cm for <i>Bacillus subtilis</i>. <br><a href="http://partsregistry.org/Part:BBa_K818000" target=_blank><font size=4 color=#FF6700><b>Easy cloning in <i>Bacillus subtilis</i> in Biobrick fashion for the first time!</b></font></a> Advantages: easy to check, BioBrick compatible, <i>E. coli</i> compatible, stable insertion into <i>Bacillus subtilis</i> chromosome.<br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Identification of spoiled meat sensors by transcriptome analysis. Usage of an innovative experimental setup which can be used to test more sensors in the future.<br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We made AmilCP and AmilGFP be expressed in <i>Bacillus subtilis</i>. These chromoproteins can be of significant value to the other <i>Bacillus subtilis</i> users in the BioBrick community.<br><br><br></p> | ||
+ | <p class=orange>Other</p> | ||
+ | <p class="marginleft"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> Smart spores activation system: inspired by "glow in the dark stick", we designed a sticker with two compartments. One compartment holds the spores, the other the nutrients; when the nutrients compartment is braked, the spores start to germinate, activating the sticker.<br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We explored the definition of spoiled meat and different ways to test this.<br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We identified various compounds present in spoiled meat by Gas Chromatography - Mass Spectrometry.<br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/d/df/IGEMGroningen2012_AD20120915_Tick.png" width=20> We made a model which gives insight on how to tweak the growth of <i>Bacillus subtilis</i> based upon literature, experimental data and flux balance analysis.<br></p> | ||
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Revision as of 02:11, 27 September 2012
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