Team:Groningen/Project
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- | body | + | <body> |
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+ | <div class="cte"> | ||
+ | <div class="ctd"> | ||
+ | <z1>Abstract</z1> | ||
+ | </div> | ||
+ | </div> | ||
+ | <table class="centertable"> | ||
+ | <tr> | ||
+ | <td class="margincell" align="right"> | ||
+ | <div class="bigcog" id="bigcogtopleft"> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150px" height="150px"> | ||
+ | </div> | ||
+ | </td> | ||
+ | <td colspan="4"> | ||
+ | <p class="nomargin"> | ||
+ | Every year, one third of global food production -1.3 billion tons of food- is thrown away, partially due to the “best before” dating system. | ||
+ | <z7>iGEM Groningen 2012</z7> seeks to provide an alternative method of assessing edibility: the <z7>Food Warden</z7>. It uses an <z7>engineered strain</z7> | ||
+ | of <i>Bacillus subtilis</i> to detect and report volatiles in spoiling meat. The introduced <z7>genetic construct</z7> uses a promoter to trigger | ||
+ | a pigment coding gene. This promoter, <z7>identified by microarray analysis</z7>, is significantly upregulated in the presence of | ||
+ | <z7>volatiles from spoiling meat</z7>. The activity of the <z7>promoter</z7> regulates the expression of the <z7>pigment reporter</z7> and will | ||
+ | be visible to the naked eye. For safe usage of the system, spores of our engineered strain are placed into one half of a semi-permeable | ||
+ | <z7>capsule</z7>, the second containing a calibrated amount of nutrients. Breaking the barrier between the two compartments allows | ||
+ | <z7>germination and growth</z7>, thereby activating the <z7>spoiling-meat sensor</z7>. | ||
- | + | </p> | |
- | + | </td> | |
- | + | <td class="margincell" align="left"> | |
- | + | <div class="bigcog" id="bigcogtopright"> | |
- | + | <img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150px" height="150px"> | |
- | + | </div> | |
- | + | </td> | |
- | + | </tr> | |
- | + | <tr> | |
- | + | <td class="margincell"> | |
- | + | <div class="cogoverlay" id="cogoverlaybottomleft"> | |
- | + | <img src="https://static.igem.org/mediawiki/2012/c/c2/Groningen2012_RR_20120909_rotting.png" width="100" height="100"> | |
- | + | </div> | |
- | + | </td> | |
- | + | <td align="left" > | |
- | + | <div class="bigcog" id="bigcogbottomleft"> | |
- | + | <img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150px" height="150px"> | |
- | + | </div> | |
- | + | </td> | |
- | + | <td align="left" > | |
- | + | <div class="cogoverlay" id="cogoverlaytopleft"> | |
- | + | <img src="https://static.igem.org/mediawiki/2012/1/15/Groningen2012_RR20120909_construct.png" width="100px" height="100px"> | |
- | + | </div> | |
- | + | </td> | |
- | + | <td align="right" > | |
- | + | <div class="cogoverlay" id="cogoverlaytopright"> | |
- | + | <img src="https://static.igem.org/mediawiki/2012/2/29/Groningen2012_RR20120909_badmeat.png" width="100px" height="100px"> | |
- | + | </div> | |
- | + | </td> | |
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- | + | <div class="bigcog" id="bigcogbottomright"> | |
- | </ | + | <img src="https://static.igem.org/mediawiki/2012/b/b6/Groningen2012_AD_20120802_BigCog.png" width="150px" height="150px"> |
- | + | </div> | |
- | <div class=" | + | </td> |
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- | < | + | <div class="cogoverlay" id="cogoverlaybottomright"> |
+ | <img src="https://static.igem.org/mediawiki/2012/e/e7/Groningen2012_RR20120909_capsule.png" width="100px" height="100px"> | ||
+ | </div> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <div class="cte2"> | ||
+ | <div class="ctd2"> | ||
+ | <a name="MainAcc"></a><z2 >Our main accomplishments</z2><br><br> | ||
</div> | </div> | ||
</div> | </div> | ||
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- | + | <li> | |
- | + | <a href="#"><img src="https://static.igem.org/mediawiki/2012/c/cb/Groningen2012_RR1capsule_break.png" width=200 /><img src="https://static.igem.org/mediawiki/2012/c/cb/Groningen2012_RR1capsule_break.png" class="preview" width=400 /></a> | |
- | + | </li> | |
- | + | </ul> | |
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- | + | <td class="accPic"> | |
- | </ | + | <ul class="hoverbox"> |
+ | <li> | ||
+ | <a href="#"><img src="https://static.igem.org/mediawiki/2012/c/c2/Groningen2012_RR_20120909_rotting.png" width=150 /><img src="https://static.igem.org/mediawiki/2012/c/c2/Groningen2012_RR_20120909_rotting.png" class="preview" width=400 /></a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
- | < | + | <tr> |
- | < | + | <td class="accCap"> |
- | < | + | <p class="small"><font color=#FF6700><br><b>1.</b></font>We designed and tested the "Sticker": a capsule in which bacteria are kept inside and volatiles can go through. We used a model to get insight on how to tweak the growth of <i>Bacillus subtilis</i>.</p> |
- | </ | + | </td> |
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- | </ | + | |
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- | + | <td class="accCap"> | |
- | + | <p class="small"><font color=#FF6700><b>2.</b></font>We explored the definition of spoiled meat and ways to check meat spoilage. We identified various compounds present in spoiled meat.<br></p> | |
+ | </td> | ||
+ | </tr> | ||
- | + | <tr> | |
- | + | <td class="accPic"> | |
- | + | <ul class="hoverbox"> | |
- | + | <li> | |
- | + | <a href="#"><img src="https://static.igem.org/mediawiki/2012/2/29/Groningen2012_RR20120909_badmeat.png" width=200 /><img src="https://static.igem.org/mediawiki/2012/2/29/Groningen2012_RR20120909_badmeat.png" class="preview" width=400 /></a> | |
- | + | </li> | |
- | + | </ul> | |
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- | + | <td class="accPic"> | |
- | </ | + | <ul class="hoverbox"> |
+ | <li> | ||
+ | <a href="#"><img src="https://static.igem.org/mediawiki/2012/2/24/Groningen2012_RRADPsac_transp.png" width=150 /><img src="https://static.igem.org/mediawiki/2012/2/24/Groningen2012_RRADPsac_transp.png" class="preview" width=400 /></a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
- | < | + | <tr> |
- | < | + | <td class="accCap"> |
- | < | + | <p class="small"><font color=#FF6700>3.</b></font> We identified spoiled meat sensors by transcriptome analysis.<br><br><br></p> |
- | < | + | </td> |
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- | </ | + | |
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- | + | <td class="accCap"> | |
- | + | <p class="small"><font color=#FF6700><b>4.</b></font> Backbone Psac-cm: easy cloning in <i>B. subtilis</i>, the BioBrick way, for the first time! It's easy to check, biobrick compatible, <i>E. coli</i> compatible, stabily inserted into the <i>B. subtilis</i> chromosome</p> | |
+ | </td> | ||
+ | </tr> | ||
- | + | <tr> | |
- | + | <td class="accPic"> | |
- | + | <ul class="hoverbox"> | |
- | + | <li> | |
- | + | <a href="#"><img src="https://static.igem.org/mediawiki/2012/f/f1/Groningen2012_RRADPigments_transp.png" width=150 /><img src="https://static.igem.org/mediawiki/2012/f/f1/Groningen2012_RRADPigments_transp.png" class="preview" width=400 /></a> | |
- | + | </li> | |
- | + | </ul> | |
- | + | </td> | |
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- | + | <td class="accPic"> | |
- | </ | + | <ul class="hoverbox"> |
+ | <li> | ||
+ | <a href="#"><img src="https://static.igem.org/mediawiki/2012/9/93/Groningen2012_ADConstruct_yellow.png" width=200 /><img src="https://static.igem.org/mediawiki/2012/9/93/Groningen2012_ADConstruct_yellow.png" class="preview" width=400 /></a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
- | < | + | <tr> |
- | < | + | <td class="accCap"> |
- | < | + | <p class="small"><font color=#FF6700><br><br><b>5.</b></font> We made AmilCP and AmilGFP be expressed in <i>Bacillus subtilis</i>.<br> These chromoproteins can be of significant value to the other <i>Bacillus subtilis</i> users in the BioBrick community.<br><br><br></p> |
- | </ | + | </td> |
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- | + | <td class="accCap"> | |
- | + | <p class="small"><font color=#FF6700><b>6.</b></font> Most importantly: we developed a construct which makes <i>Bacillus subtilis</i> sense spoiled meat and produce an output in the form of a yellow or purple pigment visible by naked eye.<br><br><br></p> | |
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
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Revision as of 02:10, 27 September 2012
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Every year, one third of global food production -1.3 billion tons of food- is thrown away, partially due to the “best before” dating system.
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2.We explored the definition of spoiled meat and ways to check meat spoilage. We identified various compounds present in spoiled meat. |
3. We identified spoiled meat sensors by transcriptome analysis. |
4. Backbone Psac-cm: easy cloning in B. subtilis, the BioBrick way, for the first time! It's easy to check, biobrick compatible, E. coli compatible, stabily inserted into the B. subtilis chromosome |
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6. Most importantly: we developed a construct which makes Bacillus subtilis sense spoiled meat and produce an output in the form of a yellow or purple pigment visible by naked eye. |