Team:Groningen/Safety

From 2012.igem.org

(Difference between revisions)
 
(44 intermediate revisions not shown)
Line 1: Line 1:
{{HeaderGroningen2012}}
{{HeaderGroningen2012}}
-
==The project==
 
-
'''Researcher safety'''
 
-
* We worked with bad meat, a versatile environment for the growth of Pseudomonas, Salmonella, E. coli and other harmful bacteria. In order to work as safely as possible, we performed our rotten meat experiments in closed bottles and used our flow cabinets when taking meat samples. All of the meat was assumed to be biological waste, so we put everything in the autoclave to ensure that our researchers worked as safe as possible.  
+
<html>
 +
<head>
 +
<style>
 +
z1 {
 +
font-size:18pt;
 +
line-height:21pt;
 +
color:white;
 +
}
 +
z5 {
 +
font-size:14pt;
 +
font-weight: bold;
 +
color: rgb(255,103,0);
 +
}
 +
.ctd {
 +
width: 180px;
 +
text-align: center;
 +
background: #000;
 +
background-color: rgba(0,0,0,0.3);
 +
padding: 5px ;
 +
margin: 30px auto;
 +
color: #FFF;
 +
}
 +
.cte {
 +
width: 210px;
 +
text-align: center;
 +
background: #000;
 +
padding-right:10px;
 +
padding-left:10px;
 +
margin: 0 auto;
 +
background-color: rgba(0,0,0,0.3);
 +
color: #FFF;
 +
}
 +
p{
 +
font-size:12pt;
 +
line-height:14pt;
 +
margin-right:150px;
 +
margin-left:150px;
 +
color: white;
 +
}
 +
a.inlink{
 +
font-size: 12pt;
 +
                line-height: 12pt;
 +
                color: rgb(255,103,0);
 +
}
 +
</style>
 +
</head>
 +
<body>
 +
<div class="cte">
 +
<div class="ctd">
 +
<z1 >General Safety</z1>
 +
</div>
 +
</div>
 +
<br>
 +
<br>
 +
<p>
 +
With the emergence of the field of Synthetic Biology, certain ethical questions must be considered.
 +
The debate on the safety and ethics of synthetic biology is still ongoing, and it is therefore no surprise
 +
that every iGEM team is obliged to consider these issues. The iGEM organization asks questions about the safety
 +
aspect of a team's project, pertaining to the safety in the lab, the environment, and the public. We tried to
 +
answer these questions to the best of our ability. Most of our team members have lab experience and are aware
 +
of the safety regulations pertaining to working in a lab with GMOs, but we decided to explore the safety issue
 +
more in depth. In order to do so, we invited two authorities to give lectures and to assist in considering the
 +
need of safety during our iGEM project: Dr. J.S. Lolkema, biological safety officer at the Rijksuniversiteit of
 +
Groningen (RUG), as well as Prof. Dr. ir.  J.D. van Elsas, member of the Netherlands Commission on Genetic Modification.
 +
<br>
 +
<br>
 +
We divided our safety page into four different sections. On the first page we focus on
 +
<a class="inlink" href="https://2012.igem.org/Team:Groningen/10com">safety in the lab</a>,
 +
we  address the rules and regulations of lab work at the RUG pertaining to researcher safety and all things concerning
 +
the lab such as: the biobricks, microorganisms and the biosafety group.
 +
<br>
 +
<br>
 +
On the  <a class="inlink" href="https://2012.igem.org/Team:Groningen/publicsafety">public safety page</a>
 +
we explore the safety of our sticker design and the public perception of the Food Warden system.
 +
<br>
 +
<br>
 +
On the <a class="inlink" href="https://2012.igem.org/Team:Groningen/environment">environmental safety page</a>
 +
we elaborate on the risk and hazards of the our genetic modification. Furthermore we address possible effects
 +
on the environment and share our thoughts on developing a system to kill the Food Warden bacterium if it were
 +
to be released into the environment or after use.
 +
<br>
 +
<br>
 +
On our last safety page we discuss <a class="inlink" href="https://2012.igem.org/Team:Groningen/foodsafety">food safety</a>.
 +
We answer questions like: "How does one define rotten meat?" and show the importance of prevention of eating spoiled meat.
 +
<br>
 +
<br>
 +
<br>
 +
There is one question from the iGEM headquarters which was hard to put into one of our safety sections, which we would like to address here.
 +
<br>
 +
<br>
 +
<z5>"Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions?
 +
How could parts, devices and systems be made even safer through biosafety engineering?"</z5>
 +
<br>
 +
<br>
 +
We think it is important to find an alternative system for the antibiotic resistance markers used nowadays in the
 +
BioBrick system. Such a selection marker could be a harmless pigment, or other systems such as the widely used
 +
Red-White or LacZ screening methods. This takes away the risk of spreading antibiotic resistance by horizontal gene transfer.
 +
<br>
 +
<br>
 +
When using proper lab techniques, safety measures, and of course common sense, a lot of possibly dangerous situations
 +
can be avoided. However, this is not always as straightforward as it seems. Every iGEM team that does wetwork has its
 +
own lab in the university, just as we do. Therefore, a random safety inspection by the iGEM organization would ideally
 +
be a good way to check if everyone does meet the basic safety regulations. However, since iGEM is a huge competition,
 +
we understand it would be troublesome to send a delegation to travel all over the world to do this. Nonetheless it could
 +
be an option to send iGEM judges or supervisors to neighboring universities, or make it obligated that iGEM teams are
 +
visited by an official Safety Officer.
 +
<br>
 +
<br>
 +
<br>
 +
</p>
 +
</body>
 +
</html>
-
* Bacillus subtilis
+
{{Template:SponsorsGroningen2012}}
-
 
+
-
* Escherichia coli
+
-
 
+
-
* Ethidium bromide is a carcinogenic compound that we used when we worked with agarose gels. To ensure our safety, we created a specific ethidium bromide region in our lab; in this area was clearly indicated that our researchers should use gloves and keep all the contaminated equipment in the EthBr area.
+
-
 
+
-
'''Public safety'''
+
-
 
+
-
During our project discussions and our presentations to the public, it became clear that the association of meat and bacteria being close to each other, is not easily accepted. That’s why decided to take extra care when we designed our indication sticker. To be absolutely sure there is no possibility that our Bacillus or its spores are able to come in the environment or on the preserved meat, we asked specialized companies to share their knowledge with us. A good idea for preventing exchange of GMO’s to the environment, is the use of nano pores.
+
-
 
+
-
But not only the sticker design ensures the safety of the public, also the nutrient composition in the sticker ensures Bacillus will only germinate and grow when the sticker is still intact.
+
-
And even when our Bacillus is exposed to a environment that is favorable for growth, the production of the pigment will ensure that the bacterium kills itself, because the pigment is toxic for Bacillus.
+
-
 
+
-
'''Environmental safety'''
+
-
 
+
-
'''The BioBricks'''
+
-
 
+
-
'''The biosafety group'''
+
-
 
+
-
'''Safety issues and the future of the iGEM competition'''
+
-
 
+
-
 
+
-
Use this page to answer the questions on the  [[Safety | safety page]].
+

Latest revision as of 21:21, 26 September 2012





General Safety


With the emergence of the field of Synthetic Biology, certain ethical questions must be considered. The debate on the safety and ethics of synthetic biology is still ongoing, and it is therefore no surprise that every iGEM team is obliged to consider these issues. The iGEM organization asks questions about the safety aspect of a team's project, pertaining to the safety in the lab, the environment, and the public. We tried to answer these questions to the best of our ability. Most of our team members have lab experience and are aware of the safety regulations pertaining to working in a lab with GMOs, but we decided to explore the safety issue more in depth. In order to do so, we invited two authorities to give lectures and to assist in considering the need of safety during our iGEM project: Dr. J.S. Lolkema, biological safety officer at the Rijksuniversiteit of Groningen (RUG), as well as Prof. Dr. ir. J.D. van Elsas, member of the Netherlands Commission on Genetic Modification.

We divided our safety page into four different sections. On the first page we focus on safety in the lab, we address the rules and regulations of lab work at the RUG pertaining to researcher safety and all things concerning the lab such as: the biobricks, microorganisms and the biosafety group.

On the public safety page we explore the safety of our sticker design and the public perception of the Food Warden system.

On the environmental safety page we elaborate on the risk and hazards of the our genetic modification. Furthermore we address possible effects on the environment and share our thoughts on developing a system to kill the Food Warden bacterium if it were to be released into the environment or after use.

On our last safety page we discuss food safety. We answer questions like: "How does one define rotten meat?" and show the importance of prevention of eating spoiled meat.


There is one question from the iGEM headquarters which was hard to put into one of our safety sections, which we would like to address here.

"Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?"

We think it is important to find an alternative system for the antibiotic resistance markers used nowadays in the BioBrick system. Such a selection marker could be a harmless pigment, or other systems such as the widely used Red-White or LacZ screening methods. This takes away the risk of spreading antibiotic resistance by horizontal gene transfer.

When using proper lab techniques, safety measures, and of course common sense, a lot of possibly dangerous situations can be avoided. However, this is not always as straightforward as it seems. Every iGEM team that does wetwork has its own lab in the university, just as we do. Therefore, a random safety inspection by the iGEM organization would ideally be a good way to check if everyone does meet the basic safety regulations. However, since iGEM is a huge competition, we understand it would be troublesome to send a delegation to travel all over the world to do this. Nonetheless it could be an option to send iGEM judges or supervisors to neighboring universities, or make it obligated that iGEM teams are visited by an official Safety Officer.


Our sponsors:

Retrieved from "http://2012.igem.org/Team:Groningen/Safety"