Team:NYMU-Taipei/ymihref=

From 2012.igem.org

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<div class="title">Human Practice</div>
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<div class="title">Extras</div>
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   <p><span class="subtitle">Meeting with NTU-Taida</span></p>
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   <p><span class="subtitle">Achievements</span></p>
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   <p><strong><em>Into the cell</em></strong>
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   <p>National Taiwan University which is the best and most historic college in Taiwan joins the iGEMcompetition first time. Both NTU and NYMU located in Taipei.  As a result, this meeting was easily held on June 1st without any  difficulty. Besides, some participants were friends or classmates during senior high school.</p>
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  <p>Unlike the traditional meeting, we, NYMU-Taipei iGEM Team, treated the friends from NTU and made the meeting like party. So, we, NYMU-Taipei team, prepared some drink and delicious food.</p>
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   <p>NTU-Taida team showed their team-work ability. I was surprised that they have good project-design, perfect bio-circuit and models. I couldn’t believe that NTU-Taida just participated in iGEM this year. Compared to National Taiwan University, National Yang Ming University is just a biomedical school which is lack of departments like economic, politics, electronic, etc. Owing to it, our report might not be more abundant than them. But I thought our team had more creative idea than them. Also, our team presentation was great.</p>
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    We construct a plasmid that contains LLO, invasin, and mRFP. Then  add the transgenic E.coli (absorbance of  0.5 at OD600, 1ml ) into the medium of amoeba (10ml) and  let stand for 1,4, 8, 12, 24 hours.<br />
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   <p>Both NTU and NYMU classmates weren&rsquo;t afraid of  asking questions to the reporters. The members of two iGEM teams shared their interest in Synthetic biology and learning. The questions from classmates let  us have more experience and learn more. It is a good activity for iGEMers to devote themselves into. I hope we can have a meetup next time as soon as possible.</p>
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  <img src="images/cdf.gif" alt=" " width="407" height="187" /></p>
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<p>
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   <p>Then we observe the amoeba with fluorescent  microscope to see if there is any E.coli inside of amoeba. We also compared the  fluorescent between amoeba cell that were co-cultured with the cell. To make  sure the E.coli really stay INSIDE of amoeba. We use centrifugation to confirm  our observation. Dictystelium discoideum in ML-5 medium should be settled with  1000 x g for 4 min and E.coli should not be settled beneath 2000 x g for 5min.  We will test this statement. If it is true, then we will spin down the liquid medium containing amoeba and E.coli at 1000 x g for 4 min. Two types of E.coli  will be tested. One can express LLO, invasin, and GFP; the other express GFP only. Discard the supernatant after centrifugation and wash the pellet with ML-5 medium. Repeat three times. Then we can use the spectrometer to compare the fluorescence in  the pellet.</p>
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   <p><strong><em>Kill-switch of  amoeba<br />
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  </em></strong>We cloned and tested  the function of zinTp. The transgenic E.coli contains zinTp+GFP, which should fluoresce  when Cd2+ exist. We added different concentration of cadmium ion into LB medium  and compared the fluorescence level of E.coli which contain zinTp+GFP, pTetR+GFP, and wildtype E.coli.
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   <p><strong><em>Tolerance to cadmium ions</em></strong><br />
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    We compared the tolerance level between non-transgenic E.coli and transgenic E.coli. We tested the growth curve of E.coli with different cadmium concentration.  </p>
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<h2 class="drawer-handle">Human Practice</h2>
 
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                <li><a title="Taiwan iGEMer Fest" href="https://2012.igem.org/Team:NYMU-Taipei/ymihpa.html">Taiwan iGEMer Fest</a></li>
 
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                <li><a title="Press Release" href="https://2012.igem.org/Team:NYMU-Taipei/ymihpb.html">Press Release</a></li>
 
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                <li><a title="Meeting with NTU-Taida" href="https://2012.igem.org/Team:NYMU-Taipei/ymihpc.html">Meeting with NTU-Taida</a></li>
 
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                <li><a title="Synthetic Biology Worldwide" href="https://2012.igem.org/Team:NYMU-Taipei/ymihpd.html">Synthetic Biology Worldwide</a></li>
 
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                <li><a title="Board Game" href="https://2012.igem.org/Team:NYMU-Taipei/ymihpe.html">Board Game</a></li>
 
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                <li><a title="Welcome Party for Freshmen" href="https://2012.igem.org/Team:NYMU-Taipei/ymihpf.html">Welcome Party for Freshmen</a></li>
 
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                <li><a title="Exhibition of iGEM in NYMU" href="https://2012.igem.org/Team:NYMU-Taipei/ymihpg.html">Exhibition of iGEM in NYMU</a></li>
 
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                <li><a title="Say Hello to Aliens !" href="https://2012.igem.org/Team:NYMU-Taipei/ymihph.html">Say Hello to Aliens !</a></li>
 
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                <li><a title="Having Fun in Hot Spring Valley" href="https://2012.igem.org/Team:NYMU-Taipei/ymihpi.html">Having Fun in Hot Spring <br />
 
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                Valley</a></li>
 
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                <li><a title="Sing our Ideas out Loud " href="https://2012.igem.org/Team:NYMU-Taipei/ymihpj.html">Sing our Ideas out Loud</a></li>
 
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          <h2 class="drawer-handle">Extras</h2>
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                <li><a title="Achievements" href="https://2012.igem.org/Team:NYMU-Taipei/ymijf.html">Achievements</a></li>
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                <li><a title="Safety"  href="https://2012.igem.org/Team:NYMU-Taipei/ymisf.html">Safety</a></li>
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                <li><a title="Collaboration"  href="https://2012.igem.org/Team:NYMU-Taipei/ymico.html">Collaboration</a></li>
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                <li><a title="Parts"  href="https://2012.igem.org/Team:NYMU-Taipei/ymiparts.html">Parts</a></li>
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            <h2 class="drawer-handle">Protocol</h2>
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                <li><a title="J774 macrophage cell line culturing" href="https://2012.igem.org/Team:NYMU-Taipei/ymip1.html">J774 macrophage cell line culturing</a></li>
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                <li><a title="Mesenchymal stem cells culturing" href="https://2012.igem.org/Team:NYMU-Taipei/ymip2.html">Mesenchymal stem cells culturing</a></li>
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                <li><a title="Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells" href="https://2012.igem.org/Team:NYMU-Taipei/ymip3.html">Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells</a></li>
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<h2 class="drawer-handle">Notebook</h2>
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                <li><a title="Week 1 ~ Week 4" href="https://2012.igem.org/Team:NYMU-Taipei/ymiw1.html">Week 1 ~ Week 4</a></li>
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                <li><a title="Week 5 ~ Week 8" href="https://2012.igem.org/Team:NYMU-Taipei/ymiw2.html">Week 5 ~ Week 8</a></li>
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                <li><a title="Week 9 ~ Week 11" href="https://2012.igem.org/Team:NYMU-Taipei/ymiw3.html">Week 9 ~ Week 11</a></li>
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            </ul>                 
            
            
            
            

Latest revision as of 21:14, 26 September 2012

NYMU iGEM

Extras

Achievements


Into the cell

We construct a plasmid that contains LLO, invasin, and mRFP. Then add the transgenic E.coli (absorbance of 0.5 at OD600, 1ml ) into the medium of amoeba (10ml) and let stand for 1,4, 8, 12, 24 hours.

Then we observe the amoeba with fluorescent microscope to see if there is any E.coli inside of amoeba. We also compared the fluorescent between amoeba cell that were co-cultured with the cell. To make sure the E.coli really stay INSIDE of amoeba. We use centrifugation to confirm our observation. Dictystelium discoideum in ML-5 medium should be settled with 1000 x g for 4 min and E.coli should not be settled beneath 2000 x g for 5min. We will test this statement. If it is true, then we will spin down the liquid medium containing amoeba and E.coli at 1000 x g for 4 min. Two types of E.coli will be tested. One can express LLO, invasin, and GFP; the other express GFP only. Discard the supernatant after centrifugation and wash the pellet with ML-5 medium. Repeat three times. Then we can use the spectrometer to compare the fluorescence in the pellet.


Kill-switch of amoeba

We cloned and tested the function of zinTp. The transgenic E.coli contains zinTp+GFP, which should fluoresce when Cd2+ exist. We added different concentration of cadmium ion into LB medium and compared the fluorescence level of E.coli which contain zinTp+GFP, pTetR+GFP, and wildtype E.coli.


Tolerance to cadmium ions

We compared the tolerance level between non-transgenic E.coli and transgenic E.coli. We tested the growth curve of E.coli with different cadmium concentration.

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