Team:Groningen/Notebook/Wetwork 10August2012

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Tom
 
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E.Coli with alsT promotor and GFP (colonies: 150-1, 300-1, 300-2, 300-3, 300-4) were grown o/n in m9 medium, illuted to OD=0.1 and grown for 2.5 hours. Samples were prepared of OD=0.05 and divided in 96-well plate. GFP fluorescence was measured o/n.
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Result:
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No GFP was seen for all the samples.
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Tom<br>
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E.Coli with alsT promotor and GFP (colonies: 150-1, 300-1, 300-2, 300-3, 300-4) were grown o/n in m9 medium, illuted to OD=0.1 and grown for 2.5 hours. Samples were prepared of OD=0.05 and divided in 96-well plate. GFP fluorescence was measured o/n. <br>
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Result:<br>
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No GFP was seen for all the samples.<br>
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Renske<br>
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<br>
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Made minimal components medium based upon Jon's modeling data ("Jon's broth")<br>
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<br>
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Glucose: 10 g/L<br>
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Potassium: 0.2 g/L<br>
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Glutamine: 0.1 g/L<br>
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<br>
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Yonathan<BR>
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Liquid culture of promising pFNR+Lycopene colony had no growth. A few subcultures show no pink color, indicating an insert in the vector. Liquid cultures prepared of these colonies.
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Latest revision as of 18:36, 26 September 2012







Tom

E.Coli with alsT promotor and GFP (colonies: 150-1, 300-1, 300-2, 300-3, 300-4) were grown o/n in m9 medium, illuted to OD=0.1 and grown for 2.5 hours. Samples were prepared of OD=0.05 and divided in 96-well plate. GFP fluorescence was measured o/n.

Result:
No GFP was seen for all the samples.

Renske

Made minimal components medium based upon Jon's modeling data ("Jon's broth")

Glucose: 10 g/L
Potassium: 0.2 g/L
Glutamine: 0.1 g/L

Yonathan
Liquid culture of promising pFNR+Lycopene colony had no growth. A few subcultures show no pink color, indicating an insert in the vector. Liquid cultures prepared of these colonies.

Back to notebook

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