Team:Groningen/Notebook/Wetwork 6August2012

From 2012.igem.org

(Difference between revisions)
 
(6 intermediate revisions not shown)
Line 1: Line 1:
{{HeaderGroningen2012}}
{{HeaderGroningen2012}}
 +
<html>
 +
<head>
 +
<style type="text/css">
 +
p.margin
 +
{
 +
font-size:12pt;
 +
line-height:14pt;
 +
color:white;
-
<div style="margin-left: 100px; margin-right:100px; color:white; font-size:12pt;">
+
margin-top:0px;
 +
margin-bottom:20px;
 +
margin-left:150px;
 +
margin-right:250px;
 +
}
-
Tom
+
</style>
 +
</head>
-
PCR alsT (150, 250, 300, 500) + GFP construct with taq-polymerase.
+
<body><br><br><br>
-
Restriction analysis for alsT (150, 250, 300, 500) + GFP with ecoRI.
+
<p class="margin">
-
 
+
Emeraldo<br>
-
Renske
+
<br>
-
 
+
Another attempt on inserting the terminator with biobrick suffix and prefix inside pSac-Cm MCS. Terminator+prefix and suffix PCR, cut both original pSac-Cm and PCR product with EcoRI and HindIII, ligation reaction, and then E.coli DH5alpha transformation.<br>
-
Counted TAMC plates + analyzed results:
+
<br>
-
 
+
Tom<br>
-
 
+
<br>
-
 
+
PCR alsT (150, 250, 300, 500) + GFP construct with taq-polymerase.<br>
-
<html>
+
Restriction analysis for alsT (150, 250, 300, 500) + GFP with ecoRI.<br>
-
<div style="position:absolute; left: 100px; top: 500px; z-index:-1;">
+
<br>
 +
Renske<br>
 +
<br>
 +
Counted TAMC plates + analyzed results:<br>
 +
<br>
<img src="https://static.igem.org/mediawiki/2012/b/b5/Groningen_RR_20120806_TAMC.png" width="450" height="320">
<img src="https://static.igem.org/mediawiki/2012/b/b5/Groningen_RR_20120806_TAMC.png" width="450" height="320">
-
</div>
+
<br><br><br>
-
</html>
+
Arjan<br>
-
 
+
<br>
-
 
+
GC-MS<br>
-
 
+
<br>
-
 
+
Did liquid injection of rotten meat volatiles using methanol as organic solvent on the HP-5 column only. This methode yielded a spectra with multiple peaks and database reference match. <br>
-
 
+
<br>
-
 
+
<A HREF="http://www.imik.org/wettelijke_context/Europese_hygienerichtlijn_en_microbiologische_criteria.pdf"><FONT COLOR=#ff6700>reference</FONT></A><br>
-
 
+
<br>
-
 
+
Renske<br>
-
 
+
<br>
-
 
+
Primer design for positivie feedback loop (with Nisa)<br>
-
 
+
<br>
-
 
+
I PlacI<br>
-
 
+
a) Prefix + PLacI_Forward<br>
-
 
+
b) homology with pRe_F + PLacI_Rv<br>
-
 
+
c) homology with pLuxR + PLuxR_Rv <br>
-
 
+
<br>
-
 
+
II P_Activator<br>
-
 
+
a) homology pLac + PRE_F<br>
-
 
+
b) homology pLac _ pLuxR<br>
-
 
+
c) homology cII with pRE_R<br>
-
 
+
d) homology LuxI + pLUxR_R<br>
-
<html>
+
<br>
-
<A HREF="http://www.imik.org/wettelijke_context/Europese_hygienerichtlijn_en_microbiologische_criteria.pdf"><FONT COLOR=#ff6700>reference</FONT></A>
+
III Activator<br>
-
</html>
+
a) homology with pRE + CII_F<br>
-
 
+
b) homolgy with pLuxI + LuxI_F/ GFP + CII_R/GFP+LuxI_R<br>
-
 
+
<br>
-
Renske
+
IV GFP<br>
-
 
+
a) CII + GFP_F<br>
-
Primer design for positivie feedback loop (with Nisa)
+
b) LuxI + GFP_R<br>
-
 
+
c) suffix _ GFP_R<br>
-
I PlacI
+
<br>
-
a) Prefix + PLacI_Forward
+
-
b) homology with pRe_F + PLacI_Rv
+
-
c) homology with pLuxR + PLuxR_Rv  
+
-
 
+
-
II P_Activator
+
-
a) homology pLac + PRE_F
+
-
b) homology pLac _ pLuxR
+
-
c) homology cII with pRE_R
+
-
d) homology LuxI + pLUxR_R
+
-
 
+
-
III Activator
+
-
a) homology with pRE + CII_F
+
-
b) homolgy with pLuxI + LuxI_F/ GFP + CII_R/GFP+LuxI_R
+
-
 
+
-
IV GFP
+
-
a) CII + GFP_F
+
-
b) LuxI + GFP_R
+
-
c) suffix _ GFP_R
+
-
 
+
-
<html>
+
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
 +
<br><br></p></body>
</html>
</html>
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
{{Template:SponsorsGroningen2012}}
{{Template:SponsorsGroningen2012}}

Latest revision as of 13:21, 26 September 2012







Emeraldo

Another attempt on inserting the terminator with biobrick suffix and prefix inside pSac-Cm MCS. Terminator+prefix and suffix PCR, cut both original pSac-Cm and PCR product with EcoRI and HindIII, ligation reaction, and then E.coli DH5alpha transformation.

Tom

PCR alsT (150, 250, 300, 500) + GFP construct with taq-polymerase.
Restriction analysis for alsT (150, 250, 300, 500) + GFP with ecoRI.

Renske

Counted TAMC plates + analyzed results:




Arjan

GC-MS

Did liquid injection of rotten meat volatiles using methanol as organic solvent on the HP-5 column only. This methode yielded a spectra with multiple peaks and database reference match.

reference

Renske

Primer design for positivie feedback loop (with Nisa)

I PlacI
a) Prefix + PLacI_Forward
b) homology with pRe_F + PLacI_Rv
c) homology with pLuxR + PLuxR_Rv

II P_Activator
a) homology pLac + PRE_F
b) homology pLac _ pLuxR
c) homology cII with pRE_R
d) homology LuxI + pLUxR_R

III Activator
a) homology with pRE + CII_F
b) homolgy with pLuxI + LuxI_F/ GFP + CII_R/GFP+LuxI_R

IV GFP
a) CII + GFP_F
b) LuxI + GFP_R
c) suffix _ GFP_R

Back to notebook

Our sponsors:

Retrieved from "http://2012.igem.org/Team:Groningen/Notebook/Wetwork_6August2012"