Under Construction

Our system consists of two different AND logic gates and an OR that derives from these ANDs. We expect three different bacillus subtillis cells to be joined by nanotubes. Through these nanotubes, outputs from the two AND cells will pass to the cell containing the OR system and will function as inputs. The first cell will produce P4 after being induced by two different signals, it will not work with only one of these signals, hence the AND logic gate. The second cell will produce LasRin the same manner. Both P4 and LasR will pass through nanotubes to the third cell. This third cell functions as an OR logic gate. If either LasR or P4 pass through the nanotubes, they will activate promoters LasB and A3 respectively. These promoters will each induce the production of GusA, so if either LasR or P4 activate one of these promoters, we will prove that both and AND logic gate and an OR logic gate took place.